##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
ApaLI AscI AspEI BbeI BbvCI
BclI BglII BseAI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BstBI
ClaI CpoI Eam1105I EcoRI EcoRV
EcoT22I FbaI HpaI KasI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RcaI RsrII SapI ScaI SfoI
SfuI SnaBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 1922 AAATTC [GA]^AATT[CT]
ApoI 1922 AAATTC [GA]^AATT[CT]
BbrPI 4193 CACGTG CAC^GTG
BciVI 4100 GGATAC GTATCC(6/5)
BsaBI 3043 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 744 ACCGGA [AT]^CCGG[AT]
Bsp1407I 4208 TGTACA T^GTACA
BsrGI 4208 TGTACA T^GTACA
Bst1107I 2965 GTATAC GTA^TAC
BstAPI 2117 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1034 GGTAACC G^GT[ACGT]ACC
BstPI 1034 GGTAACC G^GT[ACGT]ACC
BstZ17I 2965 GTATAC GTA^TAC
DraIII 2480 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1034 GGTAACC G^GT[ACGT]ACC
FseI 132 GGCCGGCC GGCCGG^CC
HindIII 788 AAGCTT A^AGCTT
MamI 3043 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2422 CCATGG C^CATGG
PflMI 415 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4193 CACGTG CAC^GTG
PmlI 4193 CACGTG CAC^GTG
PshAI 2267 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2250 GTCGAC G^TCGAC
SbfI 1499 CCTGCAGG CCTGCA^GG
SexAI 1642 ACCAGGT A^CC[AT]GGT
SfiI 1142 GGCCACCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2543 CACCGGCG C[GA]^CCGG[CT]G
SpeI 2679 ACTAGT A^CTAGT
SphI 3545 GCATGC GCATG^C
Sse8387I 1499 CCTGCAGG CCTGCA^GG
SspBI 4208 TGTACA T^GTACA
Van91I 415 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 1725 GACGTC GACGT^C
1768 GACGTC
Acc65I 2553 GGTACC G^GTACC
3110 GGTACC
AseI 3955 ATTAAT AT^TAAT
4166 ATTAAT
AsnI 3955 ATTAAT AT^TAAT
4166 ATTAAT
Asp700I 766 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3128 GAACCCTTTC
Asp718I 2553 GGTACC G^GTACC
3110 GGTACC
AspI 692 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1641 GACCAGGTC
BalI 1141 TGGCCA TGG^CCA
2054 TGGCCA
BamHI 747 GGATCC G^GATCC
1423 GGATCC
BsaAI 4193 CACGTG [CT]AC^GT[GA]
4398 TACGTG
BspLU11I 2392 ACATGT A^CATGT
3909 ACATGT
DraI 3390 TTTAAA TTT^AAA
3434 TTTAAA
DrdI 609 GACGCCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4433 GACCTGGGGGTC
KpnI 2553 GGTACC GGTAC^C
3110 GGTACC
KspI 239 CCGCGG CCGC^GG
2440 CCGCGG
MluNI 1141 TGGCCA TGG^CCA
2054 TGGCCA
MscI 1141 TGGCCA TGG^CCA
2054 TGGCCA
NruI 1990 TCGCGA TCG^CGA
2328 TCGCGA
PflFI 692 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1641 GACCAGGTC
PshBI 3955 ATTAAT AT^TAAT
4166 ATTAAT
SacI 1163 GAGCTC GAGCT^C
3690 GAGCTC
SacII 239 CCGCGG CCGC^GG
2440 CCGCGG
SmaI 15 CCCGGG CCC^GGG
3123 CCCGGG
Tth111I 692 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1641 GACCAGGTC
XmaCI 15 CCCGGG C^CCGGG
3123 CCCGGG
XmaI 15 CCCGGG C^CCGGG
3123 CCCGGG
XmnI 766 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3128 GAACCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
AocI Aor51HI ApaI AspHI AvaI
AvaII AviII AvrII BanI BanII
BbsI BbvI BcgI BcnI BfaI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII