##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AflII Aor51HI ApaI AscI
AseI AsnI Asp718I BamHI BbeI
BcgI BfrI BglII BsiWI BsmI
BspDI BspLU11I BsrDI BssHII BstAPI
BstBI BstYI ClaI CpoI DraIII
DrdI Eco47III EcoRV EcoT22I FseI
HindIII HpaI KasI KpnI KspI
MflI MluI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XhoII
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1584 GACGTC GACGT^C
AccI 3295 GTATAC GT^[AC][GT]AC
AccIII 2062 TCCGGA T^CCGGA
AclI 903 AACGTT AA^CGTT
AgeI 4956 ACCGGT A^CCGGT
AhdI 3937 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3937 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2637 CCTAGG C^CTAGG
BbrPI 4865 CACGTG CAC^GTG
BciVI 2908 GTATCC GTATCC(6/5)
BclI 4961 TGATCA T^GATCA
BglI 3016 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2637 CCTAGG C^CTAGG
BlpI 3117 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3117 GCTAAGC GC^T[ACGT]AGC
BsaBI 3471 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2062 TCCGGA T^CCGGA
BsiEI 2572 CGGCCG CG[GA][CT]^CG
Bsp1407I 4569 TGTACA T^GTACA
BspEI 2062 TCCGGA T^CCGGA
BspHI 1253 TCATGA T^CATGA
BsrGI 4569 TGTACA T^GTACA
Bst1107I 3295 GTATAC GTA^TAC
BstZ17I 3295 GTATAC GTA^TAC
CelII 3117 GCTAAGC GC^T[ACGT]AGC
EagI 2572 CGGCCG C^GGCCG
Eam1105I 3937 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2572 CGGCCG C^GGCCG
Eco52I 2572 CGGCCG C^GGCCG
EcoRI 3485 GAATTC G^AATTC
FbaI 4961 TGATCA T^GATCA
MamI 3471 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2062 TCCGGA T^CCGGA
NdeI 3285 CATATG CA^TATG
PinAI 4956 ACCGGT A^CCGGT
PmaCI 4865 CACGTG CAC^GTG
PmlI 4865 CACGTG CAC^GTG
PshAI 2031 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 903 AACGTT AA^CGTT
RcaI 1253 TCATGA T^CATGA
SspBI 4569 TGTACA T^GTACA
StuI 922 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 325 ACGTGT A^C[GA][CT]GT
4866 ACGTGT
Alw44I 2720 GTGCAC G^TGCAC
3508 GTGCAC
AocI 2774 CCTGAGG CC^T[ACGT]AGG
4060 CCTCAGG
ApaLI 2720 GTGCAC G^TGCAC
3508 GTGCAC
Asp700I 509 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
848 GAATTACTTC
AspI 2856 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4857 GACCACGTC
AviII 1372 TGCGCA TGC^GCA
3762 TGCGCA
BanI 124 GGCACC G^G[CT][GA]CC
3013 GGTGCC
BsaAI 4771 TACGTG [CT]AC^GT[GA]
4865 CACGTG
BsaWI 2062 TCCGGA [AT]^CCGG[AT]
4956 ACCGGT
BstXI 113 CCATGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2437 CCACACCAGTGG
Bsu36I 2774 CCTGAGG CC^T[ACGT]AGG
4060 CCTCAGG
DraI 3428 TTTAAA TTT^AAA
4805 TTTAAA
Eco81I 2774 CCTGAGG CC^T[ACGT]AGG
4060 CCTCAGG
FspI 1372 TGCGCA TGC^GCA
3762 TGCGCA
HincII 1192 GTCAAC GT[CT]^[GA]AC
2076 GTTGAC
HindII 1192 GTCAAC GT[CT]^[GA]AC
2076 GTTGAC
MfeI 1998 CAATTG C^AATTG
3251 CAATTG
MunI 1998 CAATTG C^AATTG
3251 CAATTG
NaeI 2604 GCCGGC GCC^GGC
2759 GCCGGC
NgoMIV 2604 GCCGGC G^CCGGC
2759 GCCGGC
NspI 1909 GCATGT [GA]CATG^[CT]
2209 GCATGT
PflFI 2856 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4857 GACCACGTC
ScaI 253 AGTACT AGT^ACT
3854 AGTACT
Tth111I 2856 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4857 GACCACGTC
XcmI 1336 CCATGGACTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2006 CCAGCCTCATCTTGG
XmnI 509 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
848 GAATTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmaCI XmaI