################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## Acc65I AflII Aor51HI ApaI AscI AseI AsnI Asp718I BamHI BbeI BcgI BfrI BglII BsiWI BsmI BspDI BspLU11I BsrDI BssHII BstAPI BstBI BstYI ClaI CpoI DraIII DrdI Eco47III EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MflI MluI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1584 GACGTC GACGT^C AccI 3295 GTATAC GT^[AC][GT]AC AccIII 2062 TCCGGA T^CCGGA AclI 903 AACGTT AA^CGTT AgeI 4956 ACCGGT A^CCGGT AhdI 3937 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 3937 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2637 CCTAGG C^CTAGG BbrPI 4865 CACGTG CAC^GTG BciVI 2908 GTATCC GTATCC(6/5) BclI 4961 TGATCA T^GATCA BglI 3016 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2637 CCTAGG C^CTAGG BlpI 3117 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3117 GCTAAGC GC^T[ACGT]AGC BsaBI 3471 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2062 TCCGGA T^CCGGA BsiEI 2572 CGGCCG CG[GA][CT]^CG Bsp1407I 4569 TGTACA T^GTACA BspEI 2062 TCCGGA T^CCGGA BspHI 1253 TCATGA T^CATGA BsrGI 4569 TGTACA T^GTACA Bst1107I 3295 GTATAC GTA^TAC BstZ17I 3295 GTATAC GTA^TAC CelII 3117 GCTAAGC GC^T[ACGT]AGC EagI 2572 CGGCCG C^GGCCG Eam1105I 3937 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2572 CGGCCG C^GGCCG Eco52I 2572 CGGCCG C^GGCCG EcoRI 3485 GAATTC G^AATTC FbaI 4961 TGATCA T^GATCA MamI 3471 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2062 TCCGGA T^CCGGA NdeI 3285 CATATG CA^TATG PinAI 4956 ACCGGT A^CCGGT PmaCI 4865 CACGTG CAC^GTG PmlI 4865 CACGTG CAC^GTG PshAI 2031 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 903 AACGTT AA^CGTT RcaI 1253 TCATGA T^CATGA SspBI 4569 TGTACA T^GTACA StuI 922 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflIII 325 ACGTGT A^C[GA][CT]GT 4866 ACGTGT Alw44I 2720 GTGCAC G^TGCAC 3508 GTGCAC AocI 2774 CCTGAGG CC^T[ACGT]AGG 4060 CCTCAGG ApaLI 2720 GTGCAC G^TGCAC 3508 GTGCAC Asp700I 509 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 848 GAATTACTTC AspI 2856 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4857 GACCACGTC AviII 1372 TGCGCA TGC^GCA 3762 TGCGCA BanI 124 GGCACC G^G[CT][GA]CC 3013 GGTGCC BsaAI 4771 TACGTG [CT]AC^GT[GA] 4865 CACGTG BsaWI 2062 TCCGGA [AT]^CCGG[AT] 4956 ACCGGT BstXI 113 CCATGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2437 CCACACCAGTGG Bsu36I 2774 CCTGAGG CC^T[ACGT]AGG 4060 CCTCAGG DraI 3428 TTTAAA TTT^AAA 4805 TTTAAA Eco81I 2774 CCTGAGG CC^T[ACGT]AGG 4060 CCTCAGG FspI 1372 TGCGCA TGC^GCA 3762 TGCGCA HincII 1192 GTCAAC GT[CT]^[GA]AC 2076 GTTGAC HindII 1192 GTCAAC GT[CT]^[GA]AC 2076 GTTGAC MfeI 1998 CAATTG C^AATTG 3251 CAATTG MunI 1998 CAATTG C^AATTG 3251 CAATTG NaeI 2604 GCCGGC GCC^GGC 2759 GCCGGC NgoMIV 2604 GCCGGC G^CCGGC 2759 GCCGGC NspI 1909 GCATGT [GA]CATG^[CT] 2209 GCATGT PflFI 2856 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4857 GACCACGTC ScaI 253 AGTACT AGT^ACT 3854 AGTACT Tth111I 2856 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4857 GACCACGTC XcmI 1336 CCATGGACTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2006 CCAGCCTCATCTTGG XmnI 509 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 848 GAATTACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmaCI XmaI