##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AflII AgeI AhdI AocI AscI
AseI AsnI AspEI AviII BcgI
BciVI BfrI BglII BsaBI BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
BssSI Bst1107I BstZ17I Bsu36I ClaI
CpoI EagI Eam1105I EclXI Eco52I
Eco81I EcoRV EcoT22I FseI FspI
MamI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshBI PvuI
RsrII SalI SapI SbfI SexAI
SfiI SgrAI SnaBI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 521 GACGTC GACGT^C
Acc65I 2143 GGTACC G^GTACC
AccIII 1522 TCCGGA T^CCGGA
AclI 6081 AACGTT AA^CGTT
Alw44I 4031 GTGCAC G^TGCAC
ApaI 14 GGGCCC GGGCC^C
ApaLI 4031 GTGCAC G^TGCAC
Asp700I 883 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2143 GGTACC G^GTACC
AvrII 2720 CCTAGG C^CTAGG
BbeI 5907 GGCGCC GGCGC^C
BbrPI 647 CACGTG CAC^GTG
BbvCI 271 GCTGAGG CCTCAGC(-5/-2)
BlnI 2720 CCTAGG C^CTAGG
BlpI 4331 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4331 GCTTAGC GC^T[ACGT]AGC
BsaAI 647 CACGTG [CT]AC^GT[GA]
BsaWI 1522 TCCGGA [AT]^CCGG[AT]
BseAI 1522 TCCGGA T^CCGGA
BsmBI 2594 CGTCTC CGTCTC(1/5)
BspEI 1522 TCCGGA T^CCGGA
BspLU11I 1681 ACATGT A^CATGT
BsrFI 993 ACCGGC [GA]^CCGG[CT]
CelII 4331 GCTTAGC GC^T[ACGT]AGC
Cfr10I 993 ACCGGC [GA]^CCGG[CT]
EcoNI 4416 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 5907 GGCGCC G^GCGCC
KpnI 2143 GGTACC GGTAC^C
KspI 193 CCGCGG CCGC^GG
MfeI 3782 CAATTG C^AATTG
MroI 1522 TCCGGA T^CCGGA
MunI 3782 CAATTG C^AATTG
NarI 5907 GGCGCC GG^CGCC
PmaCI 647 CACGTG CAC^GTG
PmlI 647 CACGTG CAC^GTG
PshAI 1503 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6081 AACGTT AA^CGTT
PvuII 1049 CAGCTG CAG^CTG
SacII 193 CCGCGG CCGC^GG
ScaI 5940 AGTACT AGT^ACT
SfoI 5907 GGCGCC GGC^GCC
XbaI 3699 TCTAGA T^CTAGA
XcmI 5559 CCAAGACTTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 883 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 194 CGCG CG^CG
5364 CGCG
AflIII 1681 ACATGT A^C[GA][CT]GT
3822 ACACGT
Aor51HI 1026 AGCGCT AGC^GCT
2431 AGCGCT
AspI 55 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3454 GACTTAGTC
BalI 292 TGGCCA TGG^CCA
5571 TGGCCA
BamHI 31 GGATCC G^GATCC
631 GGATCC
BbsI 1574 GAAGAC GAAGAC(2/6)
6097 GTCTTC
BpuAI 1574 GAAGAC GAAGAC(2/6)
6097 GTCTTC
BspHI 51 TCATGA T^CATGA
5388 TCATGA
BsrBI 35 CCGCTC CCGCTC(-3/-3)
141 GAGCGG
BstAPI 997 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1059 GCAAAATCTGC
BstBI 3442 TTCGAA TT^CGAA
6100 TTCGAA
BstEII 5489 GGTGACC G^GT[ACGT]ACC
5839 GGTCACC
BstPI 5489 GGTGACC G^GT[ACGT]ACC
5839 GGTCACC
BstUI 194 CGCG CG^CG
5364 CGCG
DrdI 86 GACATCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4960 GACTATTAAGTC
Eco47III 1026 AGCGCT AGC^GCT
2431 AGCGCT
EcoO65I 5489 GGTGACC G^GT[ACGT]ACC
5839 GGTCACC
EcoRI 370 GAATTC G^AATTC
3287 GAATTC
HgaI 306 GACGC GACGC(5/10)
4070 GCGTC
HindIII 2913 AAGCTT A^AGCTT
4373 AAGCTT
HpaI 1685 GTTAAC GTT^AAC
5172 GTTAAC
MluNI 292 TGGCCA TGG^CCA
5571 TGGCCA
MscI 292 TGGCCA TGG^CCA
5571 TGGCCA
MvnI 194 CGCG CG^CG
5364 CGCG
NspV 3442 TTCGAA TT^CGAA
6100 TTCGAA
PflFI 55 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3454 GACTTAGTC
RcaI 51 TCATGA T^CATGA
5388 TCATGA
SacI 47 GAGCTC GAGCT^C
106 GAGCTC
SfuI 3442 TTCGAA TT^CGAA
6100 TTCGAA
SmaI 1595 CCCGGG CCC^GGG
3386 CCCGGG
StuI 5913 AGGCCT AGG^CCT
6178 AGGCCT
Tth111I 55 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3454 GACTTAGTC
XmaCI 1595 CCCGGG C^CCGGG
3386 CCCGGG
XmaI 1595 CCCGGG C^CCGGG
3386 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII