################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AflII AgeI AhdI AocI AscI AseI AsnI AspEI AviII BcgI BciVI BfrI BglII BsaBI BsiEI BsiWI Bsp1407I BspDI BsrGI BssHII BssSI Bst1107I BstZ17I Bsu36I ClaI CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoRV EcoT22I FseI FspI MamI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI PvuI RsrII SalI SapI SbfI SexAI SfiI SgrAI SnaBI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 521 GACGTC GACGT^C Acc65I 2143 GGTACC G^GTACC AccIII 1522 TCCGGA T^CCGGA AclI 6081 AACGTT AA^CGTT Alw44I 4031 GTGCAC G^TGCAC ApaI 14 GGGCCC GGGCC^C ApaLI 4031 GTGCAC G^TGCAC Asp700I 883 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2143 GGTACC G^GTACC AvrII 2720 CCTAGG C^CTAGG BbeI 5907 GGCGCC GGCGC^C BbrPI 647 CACGTG CAC^GTG BbvCI 271 GCTGAGG CCTCAGC(-5/-2) BlnI 2720 CCTAGG C^CTAGG BlpI 4331 GCTTAGC GC^T[ACGT]AGC Bpu1102I 4331 GCTTAGC GC^T[ACGT]AGC BsaAI 647 CACGTG [CT]AC^GT[GA] BsaWI 1522 TCCGGA [AT]^CCGG[AT] BseAI 1522 TCCGGA T^CCGGA BsmBI 2594 CGTCTC CGTCTC(1/5) BspEI 1522 TCCGGA T^CCGGA BspLU11I 1681 ACATGT A^CATGT BsrFI 993 ACCGGC [GA]^CCGG[CT] CelII 4331 GCTTAGC GC^T[ACGT]AGC Cfr10I 993 ACCGGC [GA]^CCGG[CT] EcoNI 4416 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG KasI 5907 GGCGCC G^GCGCC KpnI 2143 GGTACC GGTAC^C KspI 193 CCGCGG CCGC^GG MfeI 3782 CAATTG C^AATTG MroI 1522 TCCGGA T^CCGGA MunI 3782 CAATTG C^AATTG NarI 5907 GGCGCC GG^CGCC PmaCI 647 CACGTG CAC^GTG PmlI 647 CACGTG CAC^GTG PshAI 1503 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 6081 AACGTT AA^CGTT PvuII 1049 CAGCTG CAG^CTG SacII 193 CCGCGG CCGC^GG ScaI 5940 AGTACT AGT^ACT SfoI 5907 GGCGCC GGC^GCC XbaI 3699 TCTAGA T^CTAGA XcmI 5559 CCAAGACTTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 883 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 194 CGCG CG^CG 5364 CGCG AflIII 1681 ACATGT A^C[GA][CT]GT 3822 ACACGT Aor51HI 1026 AGCGCT AGC^GCT 2431 AGCGCT AspI 55 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3454 GACTTAGTC BalI 292 TGGCCA TGG^CCA 5571 TGGCCA BamHI 31 GGATCC G^GATCC 631 GGATCC BbsI 1574 GAAGAC GAAGAC(2/6) 6097 GTCTTC BpuAI 1574 GAAGAC GAAGAC(2/6) 6097 GTCTTC BspHI 51 TCATGA T^CATGA 5388 TCATGA BsrBI 35 CCGCTC CCGCTC(-3/-3) 141 GAGCGG BstAPI 997 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1059 GCAAAATCTGC BstBI 3442 TTCGAA TT^CGAA 6100 TTCGAA BstEII 5489 GGTGACC G^GT[ACGT]ACC 5839 GGTCACC BstPI 5489 GGTGACC G^GT[ACGT]ACC 5839 GGTCACC BstUI 194 CGCG CG^CG 5364 CGCG DrdI 86 GACATCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4960 GACTATTAAGTC Eco47III 1026 AGCGCT AGC^GCT 2431 AGCGCT EcoO65I 5489 GGTGACC G^GT[ACGT]ACC 5839 GGTCACC EcoRI 370 GAATTC G^AATTC 3287 GAATTC HgaI 306 GACGC GACGC(5/10) 4070 GCGTC HindIII 2913 AAGCTT A^AGCTT 4373 AAGCTT HpaI 1685 GTTAAC GTT^AAC 5172 GTTAAC MluNI 292 TGGCCA TGG^CCA 5571 TGGCCA MscI 292 TGGCCA TGG^CCA 5571 TGGCCA MvnI 194 CGCG CG^CG 5364 CGCG NspV 3442 TTCGAA TT^CGAA 6100 TTCGAA PflFI 55 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3454 GACTTAGTC RcaI 51 TCATGA T^CATGA 5388 TCATGA SacI 47 GAGCTC GAGCT^C 106 GAGCTC SfuI 3442 TTCGAA TT^CGAA 6100 TTCGAA SmaI 1595 CCCGGG CCC^GGG 3386 CCCGGG StuI 5913 AGGCCT AGG^CCT 6178 AGGCCT Tth111I 55 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3454 GACTTAGTC XmaCI 1595 CCCGGG C^CCGGG 3386 CCCGGG XmaI 1595 CCCGGG C^CCGGG 3386 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbvI BclI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII