################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AgeI AhdI Aor51HI ApaI AscI AspEI AviII BamHI BbeI BbvCI BglI BglII BsiWI BsrFI BssHII BstBI Cfr10I CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI KasI MluI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PinAI PmeI PvuI RsrII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1677 GGTACC G^GTACC AccII 955 CGCG CG^CG AccIII 1217 TCCGGA T^CCGGA AclI 875 AACGTT AA^CGTT AcyI 658 GGCGTC G[GA]^CG[CT]C AflII 5557 CTTAAG C^TTAAG AseI 6004 ATTAAT AT^TAAT AsnI 6004 ATTAAT AT^TAAT Asp718I 1677 GGTACC G^GTACC AspI 238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4817 CCTAGG C^CTAGG BalI 2582 TGGCCA TGG^CCA BcgI 1458 GCAGCATATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 5557 CTTAAG C^TTAAG BlnI 4817 CCTAGG C^CTAGG BsaBI 5150 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 658 GGCGTC G[GA]^CG[CT]C BsaWI 1217 TCCGGA [AT]^CCGG[AT] BseAI 1217 TCCGGA T^CCGGA BsgI 2913 GTGCAG GTGCAG(16/14) BsiEI 3539 CGGTCG CG[GA][CT]^CG BsmBI 660 CGTCTC CGTCTC(1/5) BspDI 4090 ATCGAT AT^CGAT BspEI 1217 TCCGGA T^CCGGA BspHI 407 TCATGA T^CATGA BspMI 1604 ACCTGC ACCTGC(4/8) BsrBI 2772 GAGCGG CCGCTC(-3/-3) BssSI 3265 CTCGTG CACGAG(-5/-1) BstAPI 2148 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 955 CGCG CG^CG ClaI 4090 ATCGAT AT^CGAT DraIII 5786 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG Hin1I 658 GGCGTC G[GA]^CG[CT]C HpaI 1845 GTTAAC GTT^AAC KpnI 1677 GGTACC GGTAC^C KspI 954 CCGCGG CCGC^GG MamI 5150 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2582 TGGCCA TGG^CCA MroI 1217 TCCGGA T^CCGGA MscI 2582 TGGCCA TGG^CCA MvnI 955 CGCG CG^CG PaeR7I 2671 CTCGAG C^TCGAG PflFI 238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4346 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 4362 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 6004 ATTAAT AT^TAAT Psp1406I 875 AACGTT AA^CGTT PvuII 649 CAGCTG CAG^CTG RcaI 407 TCATGA T^CATGA SacII 954 CCGCGG CCGC^GG ScaI 5570 AGTACT AGT^ACT SpeI 5265 ACTAGT A^CTAGT SphI 4033 GCATGC GCATG^C Tth111I 238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4346 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 5370 TCTAGA T^CTAGA XhoI 2671 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AocI 3779 CCTGAGG CC^T[ACGT]AGG 3817 CCTCAGG AvaI 1980 CCCGAG C^[CT]CG[GA]G 2671 CTCGAG BbrPI 2532 CACGTG CAC^GTG 5053 CACGTG BciVI 5066 GGATAC GTATCC(6/5) 4330 GTATCC BclI 125 TGATCA T^GATCA 867 TGATCA BcnI 523 CCGGG CC^[GC]GG 675 CCGGG BsaI 779 GAGACC GGTCTC(1/5) 3279 GGTCTC BsoBI 1980 CCCGAG C^[CT]CG[GA]G 2671 CTCGAG Bsp1407I 3394 TGTACA T^GTACA 4431 TGTACA BsrGI 3394 TGTACA T^GTACA 4431 TGTACA Bst1107I 593 GTATAC GTA^TAC 5577 GTATAC BstEII 1104 GGTCACC G^GT[ACGT]ACC 1469 GGTAACC BstPI 1104 GGTCACC G^GT[ACGT]ACC 1469 GGTAACC BstXI 149 CCAAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2258 CCAGTTGATTGG BstZ17I 593 GTATAC GTA^TAC 5577 GTATAC Bsu36I 3779 CCTGAGG CC^T[ACGT]AGG 3817 CCTCAGG DrdI 259 GACATTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2401 GACTTTAGTGTC Eco81I 3779 CCTGAGG CC^T[ACGT]AGG 3817 CCTCAGG EcoO65I 1104 GGTCACC G^GT[ACGT]ACC 1469 GGTAACC EcoRI 2740 GAATTC G^AATTC 3328 GAATTC FbaI 125 TGATCA T^GATCA 867 TGATCA HaeII 969 AGCGCC [GA]GCGC^[CT] 3304 AGCGCC HgaI 659 GCGTC GACGC(5/10) 2799 GCGTC MspA1I 649 CAGCTG C[AC]G^C[GT]G 954 CCGCGG NciI 523 CCGGG CC^[GC]GG 675 CCGGG NcoI 750 CCATGG C^CATGG 2511 CCATGG NheI 2339 GCTAGC G^CTAGC 4045 GCTAGC PmaCI 2532 CACGTG CAC^GTG 5053 CACGTG PmlI 2532 CACGTG CAC^GTG 5053 CACGTG SapI 3114 GAAGAGC GCTCTTC(1/4) 4175 GAAGAGC SspBI 3394 TGTACA T^GTACA 4431 TGTACA SspI 3710 AATATT AAT^ATT 5444 AATATT StuI 3814 AGGCCT AGG^CCT 5026 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspI MunI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI