##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AgeI AhdI Aor51HI ApaI
AscI AspEI AviII BamHI BbeI
BbvCI BglI BglII BsiWI BsrFI
BssHII BstBI Cfr10I CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI KasI MluI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PinAI PmeI PvuI
RsrII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1677 GGTACC G^GTACC
AccII 955 CGCG CG^CG
AccIII 1217 TCCGGA T^CCGGA
AclI 875 AACGTT AA^CGTT
AcyI 658 GGCGTC G[GA]^CG[CT]C
AflII 5557 CTTAAG C^TTAAG
AseI 6004 ATTAAT AT^TAAT
AsnI 6004 ATTAAT AT^TAAT
Asp718I 1677 GGTACC G^GTACC
AspI 238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4817 CCTAGG C^CTAGG
BalI 2582 TGGCCA TGG^CCA
BcgI 1458 GCAGCATATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5557 CTTAAG C^TTAAG
BlnI 4817 CCTAGG C^CTAGG
BsaBI 5150 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 658 GGCGTC G[GA]^CG[CT]C
BsaWI 1217 TCCGGA [AT]^CCGG[AT]
BseAI 1217 TCCGGA T^CCGGA
BsgI 2913 GTGCAG GTGCAG(16/14)
BsiEI 3539 CGGTCG CG[GA][CT]^CG
BsmBI 660 CGTCTC CGTCTC(1/5)
BspDI 4090 ATCGAT AT^CGAT
BspEI 1217 TCCGGA T^CCGGA
BspHI 407 TCATGA T^CATGA
BspMI 1604 ACCTGC ACCTGC(4/8)
BsrBI 2772 GAGCGG CCGCTC(-3/-3)
BssSI 3265 CTCGTG CACGAG(-5/-1)
BstAPI 2148 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 955 CGCG CG^CG
ClaI 4090 ATCGAT AT^CGAT
DraIII 5786 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 658 GGCGTC G[GA]^CG[CT]C
HpaI 1845 GTTAAC GTT^AAC
KpnI 1677 GGTACC GGTAC^C
KspI 954 CCGCGG CCGC^GG
MamI 5150 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2582 TGGCCA TGG^CCA
MroI 1217 TCCGGA T^CCGGA
MscI 2582 TGGCCA TGG^CCA
MvnI 955 CGCG CG^CG
PaeR7I 2671 CTCGAG C^TCGAG
PflFI 238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4346 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4362 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6004 ATTAAT AT^TAAT
Psp1406I 875 AACGTT AA^CGTT
PvuII 649 CAGCTG CAG^CTG
RcaI 407 TCATGA T^CATGA
SacII 954 CCGCGG CCGC^GG
ScaI 5570 AGTACT AGT^ACT
SpeI 5265 ACTAGT A^CTAGT
SphI 4033 GCATGC GCATG^C
Tth111I 238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4346 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5370 TCTAGA T^CTAGA
XhoI 2671 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AocI 3779 CCTGAGG CC^T[ACGT]AGG
3817 CCTCAGG
AvaI 1980 CCCGAG C^[CT]CG[GA]G
2671 CTCGAG
BbrPI 2532 CACGTG CAC^GTG
5053 CACGTG
BciVI 5066 GGATAC GTATCC(6/5)
4330 GTATCC
BclI 125 TGATCA T^GATCA
867 TGATCA
BcnI 523 CCGGG CC^[GC]GG
675 CCGGG
BsaI 779 GAGACC GGTCTC(1/5)
3279 GGTCTC
BsoBI 1980 CCCGAG C^[CT]CG[GA]G
2671 CTCGAG
Bsp1407I 3394 TGTACA T^GTACA
4431 TGTACA
BsrGI 3394 TGTACA T^GTACA
4431 TGTACA
Bst1107I 593 GTATAC GTA^TAC
5577 GTATAC
BstEII 1104 GGTCACC G^GT[ACGT]ACC
1469 GGTAACC
BstPI 1104 GGTCACC G^GT[ACGT]ACC
1469 GGTAACC
BstXI 149 CCAAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2258 CCAGTTGATTGG
BstZ17I 593 GTATAC GTA^TAC
5577 GTATAC
Bsu36I 3779 CCTGAGG CC^T[ACGT]AGG
3817 CCTCAGG
DrdI 259 GACATTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2401 GACTTTAGTGTC
Eco81I 3779 CCTGAGG CC^T[ACGT]AGG
3817 CCTCAGG
EcoO65I 1104 GGTCACC G^GT[ACGT]ACC
1469 GGTAACC
EcoRI 2740 GAATTC G^AATTC
3328 GAATTC
FbaI 125 TGATCA T^GATCA
867 TGATCA
HaeII 969 AGCGCC [GA]GCGC^[CT]
3304 AGCGCC
HgaI 659 GCGTC GACGC(5/10)
2799 GCGTC
MspA1I 649 CAGCTG C[AC]G^C[GT]G
954 CCGCGG
NciI 523 CCGGG CC^[GC]GG
675 CCGGG
NcoI 750 CCATGG C^CATGG
2511 CCATGG
NheI 2339 GCTAGC G^CTAGC
4045 GCTAGC
PmaCI 2532 CACGTG CAC^GTG
5053 CACGTG
PmlI 2532 CACGTG CAC^GTG
5053 CACGTG
SapI 3114 GAAGAGC GCTCTTC(1/4)
4175 GAAGAGC
SspBI 3394 TGTACA T^GTACA
4431 TGTACA
SspI 3710 AATATT AAT^ATT
5444 AATATT
StuI 3814 AGGCCT AGG^CCT
5026 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI