##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       AgeI        AhdI        Aor51HI     ApaI        
AscI        AspEI       AviII       BamHI       BbeI        
BbvCI       BglI        BglII       BsiWI       BsrFI       
BssHII      BstBI       Cfr10I      CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRV       
FseI        FspI        KasI        MluI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NspV        PacI        PinAI       PmeI        PvuI        
RsrII       SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
SwaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I       1677  GGTACC               G^GTACC
AccII         955  CGCG                 CG^CG
AccIII       1217  TCCGGA               T^CCGGA
AclI          875  AACGTT               AA^CGTT
AcyI          658  GGCGTC               G[GA]^CG[CT]C
AflII        5557  CTTAAG               C^TTAAG
AseI         6004  ATTAAT               AT^TAAT
AsnI         6004  ATTAAT               AT^TAAT
Asp718I      1677  GGTACC               G^GTACC
AspI          238  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        4817  CCTAGG               C^CTAGG
BalI         2582  TGGCCA               TGG^CCA
BcgI         1458  GCAGCATATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         5557  CTTAAG               C^TTAAG
BlnI         4817  CCTAGG               C^CTAGG
BsaBI        5150  GATGGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         658  GGCGTC               G[GA]^CG[CT]C
BsaWI        1217  TCCGGA               [AT]^CCGG[AT]
BseAI        1217  TCCGGA               T^CCGGA
BsgI         2913  GTGCAG               GTGCAG(16/14)
BsiEI        3539  CGGTCG               CG[GA][CT]^CG
BsmBI         660  CGTCTC               CGTCTC(1/5)
BspDI        4090  ATCGAT               AT^CGAT
BspEI        1217  TCCGGA               T^CCGGA
BspHI         407  TCATGA               T^CATGA
BspMI        1604  ACCTGC               ACCTGC(4/8)
BsrBI        2772  GAGCGG               CCGCTC(-3/-3)
BssSI        3265  CTCGTG               CACGAG(-5/-1)
BstAPI       2148  GCAAAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         955  CGCG                 CG^CG
ClaI         4090  ATCGAT               AT^CGAT
DraIII       5786  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Hin1I         658  GGCGTC               G[GA]^CG[CT]C
HpaI         1845  GTTAAC               GTT^AAC
KpnI         1677  GGTACC               GGTAC^C
KspI          954  CCGCGG               CCGC^GG
MamI         5150  GATGGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2582  TGGCCA               TGG^CCA
MroI         1217  TCCGGA               T^CCGGA
MscI         2582  TGGCCA               TGG^CCA
MvnI          955  CGCG                 CG^CG
PaeR7I       2671  CTCGAG               C^TCGAG
PflFI         238  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        4346  CCATGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        4362  GACACCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        6004  ATTAAT               AT^TAAT
Psp1406I      875  AACGTT               AA^CGTT
PvuII         649  CAGCTG               CAG^CTG
RcaI          407  TCATGA               T^CATGA
SacII         954  CCGCGG               CCGC^GG
ScaI         5570  AGTACT               AGT^ACT
SpeI         5265  ACTAGT               A^CTAGT
SphI         4033  GCATGC               GCATG^C
Tth111I       238  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       4346  CCATGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         5370  TCTAGA               T^CTAGA
XhoI         2671  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AocI         3779  CCTGAGG              CC^T[ACGT]AGG
             3817  CCTCAGG             
AvaI         1980  CCCGAG               C^[CT]CG[GA]G
             2671  CTCGAG              
BbrPI        2532  CACGTG               CAC^GTG
             5053  CACGTG              
BciVI        5066  GGATAC               GTATCC(6/5)
             4330  GTATCC              
BclI          125  TGATCA               T^GATCA
              867  TGATCA              
BcnI          523  CCGGG                CC^[GC]GG
              675  CCGGG               
BsaI          779  GAGACC               GGTCTC(1/5)
             3279  GGTCTC              
BsoBI        1980  CCCGAG               C^[CT]CG[GA]G
             2671  CTCGAG              
Bsp1407I     3394  TGTACA               T^GTACA
             4431  TGTACA              
BsrGI        3394  TGTACA               T^GTACA
             4431  TGTACA              
Bst1107I      593  GTATAC               GTA^TAC
             5577  GTATAC              
BstEII       1104  GGTCACC              G^GT[ACGT]ACC
             1469  GGTAACC             
BstPI        1104  GGTCACC              G^GT[ACGT]ACC
             1469  GGTAACC             
BstXI         149  CCAAGAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2258  CCAGTTGATTGG        
BstZ17I       593  GTATAC               GTA^TAC
             5577  GTATAC              
Bsu36I       3779  CCTGAGG              CC^T[ACGT]AGG
             3817  CCTCAGG             
DrdI          259  GACATTCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2401  GACTTTAGTGTC        
Eco81I       3779  CCTGAGG              CC^T[ACGT]AGG
             3817  CCTCAGG             
EcoO65I      1104  GGTCACC              G^GT[ACGT]ACC
             1469  GGTAACC             
EcoRI        2740  GAATTC               G^AATTC
             3328  GAATTC              
FbaI          125  TGATCA               T^GATCA
              867  TGATCA              
HaeII         969  AGCGCC               [GA]GCGC^[CT]
             3304  AGCGCC              
HgaI          659  GCGTC                GACGC(5/10)
             2799  GCGTC               
MspA1I        649  CAGCTG               C[AC]G^C[GT]G
              954  CCGCGG              
NciI          523  CCGGG                CC^[GC]GG
              675  CCGGG               
NcoI          750  CCATGG               C^CATGG
             2511  CCATGG              
NheI         2339  GCTAGC               G^CTAGC
             4045  GCTAGC              
PmaCI        2532  CACGTG               CAC^GTG
             5053  CACGTG              
PmlI         2532  CACGTG               CAC^GTG
             5053  CACGTG              
SapI         3114  GAAGAGC              GCTCTTC(1/4)
             4175  GAAGAGC             
SspBI        3394  TGTACA               T^GTACA
             4431  TGTACA              
SspI         3710  AATATT               AAT^ATT
             5444  AATATT              
StuI         3814  AGGCCT               AGG^CCT
             5026  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaLI       
ApoI        Asp700I     AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspLU11I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MfeI        MflI        MnlI        
MseI        MslI        MspI        MunI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PpuMI       PstI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SexAI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI