################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AccI AflII AgeI Aor51HI AscI AseI AsnI Asp718I BcgI BfrI BglII BsaBI BsiWI Bsp1407I BspDI BspHI BsrGI Bst1107I BstZ17I ClaI CpoI DrdI Eco47III EcoRV FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI PacI PinAI PmeI PpuMI PshBI PvuI RcaI RsrII SacII SalI ScaI SexAI SgrAI SnaBI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AclI 4018 AACGTT AA^CGTT AhdI 1469 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1526 GTGCAC G^TGCAC AocI 2525 CCTGAGG CC^T[ACGT]AGG ApaLI 1526 GTGCAC G^TGCAC Asp700I 1373 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1469 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3617 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1230 TGCGCA TGC^GCA BbrPI 977 CACGTG CAC^GTG BbsI 1672 GAAGAC GAAGAC(2/6) BciVI 3291 GGATAC GTATCC(6/5) BclI 2448 TGATCA T^GATCA BpuAI 1672 GAAGAC GAAGAC(2/6) BsaAI 977 CACGTG [CT]AC^GT[GA] BsaI 3282 GAGACC GGTCTC(1/5) BspLU11I 434 ACATGT A^CATGT BsrDI 688 CATTGC GCAATG(2/0) BsrFI 2793 GCCGGC [GA]^CCGG[CT] BssHII 104 GCGCGC G^CGCGC BstBI 555 TTCGAA TT^CGAA Bsu36I 2525 CCTGAGG CC^T[ACGT]AGG Cfr10I 2793 GCCGGC [GA]^CCGG[CT] DraIII 974 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1469 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2525 CCTGAGG CC^T[ACGT]AGG EcoRI 1438 GAATTC G^AATTC EcoT22I 1375 ATGCAT ATGCA^T FbaI 2448 TGATCA T^GATCA FspI 1230 TGCGCA TGC^GCA NaeI 2793 GCCGGC GCC^GGC NgoMIV 2793 GCCGGC G^CCGGC NsiI 1375 ATGCAT ATGCA^T NspV 555 TTCGAA TT^CGAA PflFI 3617 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 977 CACGTG CAC^GTG PmlI 977 CACGTG CAC^GTG PshAI 173 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 4018 AACGTT AA^CGTT SacI 4185 GAGCTC GAGCT^C SfiI 1610 GGCCCCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 555 TTCGAA TT^CGAA SpeI 1489 ACTAGT A^CTAGT SphI 4254 GCATGC GCATG^C Tth111I 3617 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 3329 CCACTGCACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1373 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccIII 1326 TCCGGA T^CCGGA 1998 TCCGGA AcsI 1438 GAATTC [GA]^AATT[CT] 4106 AAATTT AflIII 434 ACATGT A^C[GA][CT]GT 1100 ACACGT ApoI 1438 GAATTC [GA]^AATT[CT] 4106 AAATTT AvrII 1483 CCTAGG C^CTAGG 3296 CCTAGG BamHI 1576 GGATCC G^GATCC 1914 GGATCC BbvCI 3943 CCTCAGC CCTCAGC(-5/-2) 520 GCTGAGG BlnI 1483 CCTAGG C^CTAGG 3296 CCTAGG BseAI 1326 TCCGGA T^CCGGA 1998 TCCGGA BspEI 1326 TCCGGA T^CCGGA 1998 TCCGGA BspMI 2229 ACCTGC ACCTGC(4/8) 2253 ACCTGC BssSI 1529 CACGAG CACGAG(-5/-1) 1727 CTCGTG BstAPI 1930 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4213 GCATGAGGTGC BstEII 231 GGTTACC G^GT[ACGT]ACC 2418 GGTCACC BstPI 231 GGTTACC G^GT[ACGT]ACC 2418 GGTCACC BstXI 2625 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3463 CCAGGGACATGG DraI 3454 TTTAAA TTT^AAA 3834 TTTAAA EagI 112 CGGCCG C^GGCCG 1618 CGGCCG EclXI 112 CGGCCG C^GGCCG 1618 CGGCCG Eco52I 112 CGGCCG C^GGCCG 1618 CGGCCG EcoNI 2188 CCTGCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2659 CCTGTTGCAGG EcoO65I 231 GGTTACC G^GT[ACGT]ACC 2418 GGTCACC MroI 1326 TCCGGA T^CCGGA 1998 TCCGGA NcoI 3304 CCATGG C^CATGG 3676 CCATGG PaeR7I 1299 CTCGAG C^TCGAG 3105 CTCGAG PflMI 1174 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1465 CCAAGACTTGG PvuII 1344 CAGCTG CAG^CTG 4329 CAGCTG SbfI 1552 CCTGCAGG CCTGCA^GG 2476 CCTGCAGG Sse8387I 1552 CCTGCAGG CCTGCA^GG 2476 CCTGCAGG Van91I 1174 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1465 CCAAGACTTGG XhoI 1299 CTCGAG C^TCGAG 3105 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI