##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
BcgI BfrI BglII BsaBI BsiWI
Bsp1407I BspDI BspHI BsrGI Bst1107I
BstZ17I ClaI CpoI DrdI Eco47III
EcoRV FseI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI PacI PinAI PmeI PpuMI
PshBI PvuI RcaI RsrII SacII
SalI ScaI SexAI SgrAI SnaBI
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 4018 AACGTT AA^CGTT
AhdI 1469 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1526 GTGCAC G^TGCAC
AocI 2525 CCTGAGG CC^T[ACGT]AGG
ApaLI 1526 GTGCAC G^TGCAC
Asp700I 1373 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1469 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3617 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1230 TGCGCA TGC^GCA
BbrPI 977 CACGTG CAC^GTG
BbsI 1672 GAAGAC GAAGAC(2/6)
BciVI 3291 GGATAC GTATCC(6/5)
BclI 2448 TGATCA T^GATCA
BpuAI 1672 GAAGAC GAAGAC(2/6)
BsaAI 977 CACGTG [CT]AC^GT[GA]
BsaI 3282 GAGACC GGTCTC(1/5)
BspLU11I 434 ACATGT A^CATGT
BsrDI 688 CATTGC GCAATG(2/0)
BsrFI 2793 GCCGGC [GA]^CCGG[CT]
BssHII 104 GCGCGC G^CGCGC
BstBI 555 TTCGAA TT^CGAA
Bsu36I 2525 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2793 GCCGGC [GA]^CCGG[CT]
DraIII 974 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1469 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2525 CCTGAGG CC^T[ACGT]AGG
EcoRI 1438 GAATTC G^AATTC
EcoT22I 1375 ATGCAT ATGCA^T
FbaI 2448 TGATCA T^GATCA
FspI 1230 TGCGCA TGC^GCA
NaeI 2793 GCCGGC GCC^GGC
NgoMIV 2793 GCCGGC G^CCGGC
NsiI 1375 ATGCAT ATGCA^T
NspV 555 TTCGAA TT^CGAA
PflFI 3617 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 977 CACGTG CAC^GTG
PmlI 977 CACGTG CAC^GTG
PshAI 173 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4018 AACGTT AA^CGTT
SacI 4185 GAGCTC GAGCT^C
SfiI 1610 GGCCCCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 555 TTCGAA TT^CGAA
SpeI 1489 ACTAGT A^CTAGT
SphI 4254 GCATGC GCATG^C
Tth111I 3617 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3329 CCACTGCACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1373 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1326 TCCGGA T^CCGGA
1998 TCCGGA
AcsI 1438 GAATTC [GA]^AATT[CT]
4106 AAATTT
AflIII 434 ACATGT A^C[GA][CT]GT
1100 ACACGT
ApoI 1438 GAATTC [GA]^AATT[CT]
4106 AAATTT
AvrII 1483 CCTAGG C^CTAGG
3296 CCTAGG
BamHI 1576 GGATCC G^GATCC
1914 GGATCC
BbvCI 3943 CCTCAGC CCTCAGC(-5/-2)
520 GCTGAGG
BlnI 1483 CCTAGG C^CTAGG
3296 CCTAGG
BseAI 1326 TCCGGA T^CCGGA
1998 TCCGGA
BspEI 1326 TCCGGA T^CCGGA
1998 TCCGGA
BspMI 2229 ACCTGC ACCTGC(4/8)
2253 ACCTGC
BssSI 1529 CACGAG CACGAG(-5/-1)
1727 CTCGTG
BstAPI 1930 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4213 GCATGAGGTGC
BstEII 231 GGTTACC G^GT[ACGT]ACC
2418 GGTCACC
BstPI 231 GGTTACC G^GT[ACGT]ACC
2418 GGTCACC
BstXI 2625 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3463 CCAGGGACATGG
DraI 3454 TTTAAA TTT^AAA
3834 TTTAAA
EagI 112 CGGCCG C^GGCCG
1618 CGGCCG
EclXI 112 CGGCCG C^GGCCG
1618 CGGCCG
Eco52I 112 CGGCCG C^GGCCG
1618 CGGCCG
EcoNI 2188 CCTGCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2659 CCTGTTGCAGG
EcoO65I 231 GGTTACC G^GT[ACGT]ACC
2418 GGTCACC
MroI 1326 TCCGGA T^CCGGA
1998 TCCGGA
NcoI 3304 CCATGG C^CATGG
3676 CCATGG
PaeR7I 1299 CTCGAG C^TCGAG
3105 CTCGAG
PflMI 1174 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCAAGACTTGG
PvuII 1344 CAGCTG CAG^CTG
4329 CAGCTG
SbfI 1552 CCTGCAGG CCTGCA^GG
2476 CCTGCAGG
Sse8387I 1552 CCTGCAGG CCTGCA^GG
2476 CCTGCAGG
Van91I 1174 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCAAGACTTGG
XhoI 1299 CTCGAG C^TCGAG
3105 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI