################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII BbeI BbrPI BfrI BglI BsaAI BsaBI BsiWI Bsp1407I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI FspI KasI KpnI KspI MamI MluI MvnI NarI NdeI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 1456 GTCTAC GT^[AC][GT]AC Alw44I 1432 GTGCAC G^TGCAC AocI 2683 CCTCAGG CC^T[ACGT]AGG ApaI 1700 GGGCCC GGGCC^C ApaLI 1432 GTGCAC G^TGCAC AvrII 4488 CCTAGG C^CTAGG BalI 3216 TGGCCA TGG^CCA BamHI 1363 GGATCC G^GATCC BcgI 436 GCACTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3033 GGATAC GTATCC(6/5) BclI 3596 TGATCA T^GATCA BlnI 4488 CCTAGG C^CTAGG BsmBI 1214 CGTCTC CGTCTC(1/5) BsmI 3512 GCATTC GAATGC(1/-1) BspDI 652 ATCGAT AT^CGAT BstEII 1751 GGTCACC G^GT[ACGT]ACC BstPI 1751 GGTCACC G^GT[ACGT]ACC BstXI 383 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2683 CCTCAGG CC^T[ACGT]AGG ClaI 652 ATCGAT AT^CGAT DraIII 3607 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2752 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2683 CCTCAGG CC^T[ACGT]AGG EcoO65I 1751 GGTCACC G^GT[ACGT]ACC EcoRV 2314 GATATC GAT^ATC EcoT22I 4541 ATGCAT ATGCA^T FbaI 3596 TGATCA T^GATCA FseI 3411 GGCCGGCC GGCCGG^CC HpaI 628 GTTAAC GTT^AAC MluNI 3216 TGGCCA TGG^CCA MscI 3216 TGGCCA TGG^CCA NaeI 3412 GCCGGC GCC^GGC NgoMIV 3412 GCCGGC G^CCGGC NheI 1222 GCTAGC G^CTAGC NsiI 4541 ATGCAT ATGCA^T PaeR7I 4580 CTCGAG C^TCGAG PvuI 477 CGATCG CGAT^CG SapI 933 GAAGAGC GCTCTTC(1/4) SexAI 3045 ACCAGGT A^CC[AT]GGT SmaI 3373 CCCGGG CCC^GGG SspI 3350 AATATT AAT^ATT XhoI 4580 CTCGAG C^TCGAG XmaCI 3373 CCCGGG C^CCGGG XmaI 3373 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 223 TCCGGA T^CCGGA 1515 TCCGGA AcyI 825 GGCGTC G[GA]^CG[CT]C 1573 GGCGTC AfaI 88 GTAC GT^AC 324 GTAC AflIII 1168 ACATGT A^C[GA][CT]GT 1742 ACATGT Asp700I 498 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4891 GAACACATTC BsaHI 825 GGCGTC G[GA]^CG[CT]C 1573 GGCGTC BsaWI 223 TCCGGA [AT]^CCGG[AT] 1515 TCCGGA BseAI 223 TCCGGA T^CCGGA 1515 TCCGGA BsiEI 477 CGATCG CG[GA][CT]^CG 690 CGACCG BspEI 223 TCCGGA T^CCGGA 1515 TCCGGA BspLU11I 1168 ACATGT A^CATGT 1742 ACATGT BsrDI 4091 CATTGC GCAATG(2/0) 4784 CATTGC BstAPI 485 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2079 GCACACCTTGC CfoI 1684 GCGC GCG^C 3698 GCGC DraI 2014 TTTAAA TTT^AAA 4754 TTTAAA HaeII 1683 AGCGCC [GA]GCGC^[CT] 3697 GGCGCT HhaI 1684 GCGC GCG^C 3698 GCGC Hin1I 825 GGCGTC G[GA]^CG[CT]C 1573 GGCGTC HinP1I 1684 GCGC G^CGC 3698 GCGC MaeII 1668 ACGT A^CGT 4386 ACGT MfeI 46 CAATTG C^AATTG 2743 CAATTG MroI 223 TCCGGA T^CCGGA 1515 TCCGGA MunI 46 CAATTG C^AATTG 2743 CAATTG NcoI 3302 CCATGG C^CATGG 4510 CCATGG NspI 1168 ACATGT [GA]CATG^[CT] 1742 ACATGT PflMI 151 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4505 CCACCCCATGG RsaI 88 GTAC GT^AC 324 GTAC SacI 1158 GAGCTC GAGCT^C 4563 GAGCTC Van91I 151 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4505 CCACCCCATGG XbaI 3480 TCTAGA T^CTAGA 3582 TCTAGA XcmI 1473 CCACTGTGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2554 CCAAAGCCAGCTTGG XmnI 498 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4891 GAACACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RcaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII