##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII BbeI
BbrPI BfrI BglI BsaAI BsaBI
BsiWI Bsp1407I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FspI KasI KpnI
KspI MamI MluI MvnI NarI
NdeI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1456 GTCTAC GT^[AC][GT]AC
Alw44I 1432 GTGCAC G^TGCAC
AocI 2683 CCTCAGG CC^T[ACGT]AGG
ApaI 1700 GGGCCC GGGCC^C
ApaLI 1432 GTGCAC G^TGCAC
AvrII 4488 CCTAGG C^CTAGG
BalI 3216 TGGCCA TGG^CCA
BamHI 1363 GGATCC G^GATCC
BcgI 436 GCACTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3033 GGATAC GTATCC(6/5)
BclI 3596 TGATCA T^GATCA
BlnI 4488 CCTAGG C^CTAGG
BsmBI 1214 CGTCTC CGTCTC(1/5)
BsmI 3512 GCATTC GAATGC(1/-1)
BspDI 652 ATCGAT AT^CGAT
BstEII 1751 GGTCACC G^GT[ACGT]ACC
BstPI 1751 GGTCACC G^GT[ACGT]ACC
BstXI 383 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2683 CCTCAGG CC^T[ACGT]AGG
ClaI 652 ATCGAT AT^CGAT
DraIII 3607 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2752 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2683 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1751 GGTCACC G^GT[ACGT]ACC
EcoRV 2314 GATATC GAT^ATC
EcoT22I 4541 ATGCAT ATGCA^T
FbaI 3596 TGATCA T^GATCA
FseI 3411 GGCCGGCC GGCCGG^CC
HpaI 628 GTTAAC GTT^AAC
MluNI 3216 TGGCCA TGG^CCA
MscI 3216 TGGCCA TGG^CCA
NaeI 3412 GCCGGC GCC^GGC
NgoMIV 3412 GCCGGC G^CCGGC
NheI 1222 GCTAGC G^CTAGC
NsiI 4541 ATGCAT ATGCA^T
PaeR7I 4580 CTCGAG C^TCGAG
PvuI 477 CGATCG CGAT^CG
SapI 933 GAAGAGC GCTCTTC(1/4)
SexAI 3045 ACCAGGT A^CC[AT]GGT
SmaI 3373 CCCGGG CCC^GGG
SspI 3350 AATATT AAT^ATT
XhoI 4580 CTCGAG C^TCGAG
XmaCI 3373 CCCGGG C^CCGGG
XmaI 3373 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 223 TCCGGA T^CCGGA
1515 TCCGGA
AcyI 825 GGCGTC G[GA]^CG[CT]C
1573 GGCGTC
AfaI 88 GTAC GT^AC
324 GTAC
AflIII 1168 ACATGT A^C[GA][CT]GT
1742 ACATGT
Asp700I 498 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4891 GAACACATTC
BsaHI 825 GGCGTC G[GA]^CG[CT]C
1573 GGCGTC
BsaWI 223 TCCGGA [AT]^CCGG[AT]
1515 TCCGGA
BseAI 223 TCCGGA T^CCGGA
1515 TCCGGA
BsiEI 477 CGATCG CG[GA][CT]^CG
690 CGACCG
BspEI 223 TCCGGA T^CCGGA
1515 TCCGGA
BspLU11I 1168 ACATGT A^CATGT
1742 ACATGT
BsrDI 4091 CATTGC GCAATG(2/0)
4784 CATTGC
BstAPI 485 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2079 GCACACCTTGC
CfoI 1684 GCGC GCG^C
3698 GCGC
DraI 2014 TTTAAA TTT^AAA
4754 TTTAAA
HaeII 1683 AGCGCC [GA]GCGC^[CT]
3697 GGCGCT
HhaI 1684 GCGC GCG^C
3698 GCGC
Hin1I 825 GGCGTC G[GA]^CG[CT]C
1573 GGCGTC
HinP1I 1684 GCGC G^CGC
3698 GCGC
MaeII 1668 ACGT A^CGT
4386 ACGT
MfeI 46 CAATTG C^AATTG
2743 CAATTG
MroI 223 TCCGGA T^CCGGA
1515 TCCGGA
MunI 46 CAATTG C^AATTG
2743 CAATTG
NcoI 3302 CCATGG C^CATGG
4510 CCATGG
NspI 1168 ACATGT [GA]CATG^[CT]
1742 ACATGT
PflMI 151 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4505 CCACCCCATGG
RsaI 88 GTAC GT^AC
324 GTAC
SacI 1158 GAGCTC GAGCT^C
4563 GAGCTC
Van91I 151 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4505 CCACCCCATGG
XbaI 3480 TCTAGA T^CTAGA
3582 TCTAGA
XcmI 1473 CCACTGTGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2554 CCAAAGCCAGCTTGG
XmnI 498 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4891 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflFI PleI PpuMI PstI
PvuII RcaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII