################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccI AclI AflII AgeI AhdI Alw44I ApaLI AscI AspEI AspI AviII BbeI BbrPI BcgI BciVI BfrI BsaBI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI FseI FspI HpaI KasI KspI MamI MluI NarI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI Sse8387I SspBI StuI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 2882 GACGTC GACGT^C Acc65I 3801 GGTACC G^GTACC AccIII 2343 TCCGGA T^CCGGA AocI 5584 CCTGAGG CC^T[ACGT]AGG Aor51HI 1368 AGCGCT AGC^GCT ApaI 4499 GGGCCC GGGCC^C AseI 4028 ATTAAT AT^TAAT AsnI 4028 ATTAAT AT^TAAT Asp718I 3801 GGTACC G^GTACC AvrII 3937 CCTAGG C^CTAGG BglII 1318 AGATCT A^GATCT BlnI 3937 CCTAGG C^CTAGG BsaWI 2343 TCCGGA [AT]^CCGG[AT] BseAI 2343 TCCGGA T^CCGGA BsmBI 4739 GAGACG CGTCTC(1/5) BspEI 2343 TCCGGA T^CCGGA BspHI 3370 TCATGA T^CATGA BsrBI 3350 GAGCGG CCGCTC(-3/-3) BstBI 3401 TTCGAA TT^CGAA Bsu36I 5584 CCTGAGG CC^T[ACGT]AGG DrdI 956 GACCTGTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1368 AGCGCT AGC^GCT Eco81I 5584 CCTGAGG CC^T[ACGT]AGG EcoNI 4795 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1867 GATATC GAT^ATC HaeII 1368 AGCGCT [GA]GCGC^[CT] KpnI 3801 GGTACC GGTAC^C MroI 2343 TCCGGA T^CCGGA NdeI 2295 CATATG CA^TATG NheI 778 GCTAGC G^CTAGC NspV 3401 TTCGAA TT^CGAA PaeR7I 2398 CTCGAG C^TCGAG PshBI 4028 ATTAAT AT^TAAT RcaI 3370 TCATGA T^CATGA SexAI 3030 ACCAGGT A^CC[AT]GGT SfuI 3401 TTCGAA TT^CGAA SnaBI 3737 TACGTA TAC^GTA SpeI 3725 ACTAGT A^CTAGT SspI 1225 AATATT AAT^ATT XhoI 2398 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflIII 1191 ACGTGT A^C[GA][CT]GT 3450 ACGTGT AlwNI 24 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 4557 CAGAGGCTG BsrDI 1084 CATTGC GCAATG(2/0) 3851 GCAATG BstXI 2972 CCAGTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3580 CCAGAGTGATGG DraIII 700 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG 5789 CACGGGGTG MfeI 362 CAATTG C^AATTG 1604 CAATTG MunI 362 CAATTG C^AATTG 1604 CAATTG NaeI 2830 GCCGGC GCC^GGC 4907 GCCGGC NgoMIV 2830 GCCGGC G^CCGGC 4907 GCCGGC PpuMI 638 GGGTCCT [GA]G^G[AT]CC[CT] 4567 GGGTCCT SmaI 3483 CCCGGG CCC^GGG 4589 CCCGGG SphI 459 GCATGC GCATG^C 1336 GCATGC XcmI 4855 CCACACACCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5423 CCACTGTTACTGTGG XmaCI 3483 CCCGGG C^CCGGG 4589 CCCGGG XmaI 3483 CCCGGG C^CCGGG 4589 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI