##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Alw44I ApaLI AscI AspEI AspI
AviII BbeI BbrPI BcgI BciVI
BfrI BsaBI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRI
FseI FspI HpaI KasI KspI
MamI MluI NarI NotI NruI
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
Sse8387I SspBI StuI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2882 GACGTC GACGT^C
Acc65I 3801 GGTACC G^GTACC
AccIII 2343 TCCGGA T^CCGGA
AocI 5584 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1368 AGCGCT AGC^GCT
ApaI 4499 GGGCCC GGGCC^C
AseI 4028 ATTAAT AT^TAAT
AsnI 4028 ATTAAT AT^TAAT
Asp718I 3801 GGTACC G^GTACC
AvrII 3937 CCTAGG C^CTAGG
BglII 1318 AGATCT A^GATCT
BlnI 3937 CCTAGG C^CTAGG
BsaWI 2343 TCCGGA [AT]^CCGG[AT]
BseAI 2343 TCCGGA T^CCGGA
BsmBI 4739 GAGACG CGTCTC(1/5)
BspEI 2343 TCCGGA T^CCGGA
BspHI 3370 TCATGA T^CATGA
BsrBI 3350 GAGCGG CCGCTC(-3/-3)
BstBI 3401 TTCGAA TT^CGAA
Bsu36I 5584 CCTGAGG CC^T[ACGT]AGG
DrdI 956 GACCTGTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1368 AGCGCT AGC^GCT
Eco81I 5584 CCTGAGG CC^T[ACGT]AGG
EcoNI 4795 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1867 GATATC GAT^ATC
HaeII 1368 AGCGCT [GA]GCGC^[CT]
KpnI 3801 GGTACC GGTAC^C
MroI 2343 TCCGGA T^CCGGA
NdeI 2295 CATATG CA^TATG
NheI 778 GCTAGC G^CTAGC
NspV 3401 TTCGAA TT^CGAA
PaeR7I 2398 CTCGAG C^TCGAG
PshBI 4028 ATTAAT AT^TAAT
RcaI 3370 TCATGA T^CATGA
SexAI 3030 ACCAGGT A^CC[AT]GGT
SfuI 3401 TTCGAA TT^CGAA
SnaBI 3737 TACGTA TAC^GTA
SpeI 3725 ACTAGT A^CTAGT
SspI 1225 AATATT AAT^ATT
XhoI 2398 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 1191 ACGTGT A^C[GA][CT]GT
3450 ACGTGT
AlwNI 24 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
4557 CAGAGGCTG
BsrDI 1084 CATTGC GCAATG(2/0)
3851 GCAATG
BstXI 2972 CCAGTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3580 CCAGAGTGATGG
DraIII 700 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5789 CACGGGGTG
MfeI 362 CAATTG C^AATTG
1604 CAATTG
MunI 362 CAATTG C^AATTG
1604 CAATTG
NaeI 2830 GCCGGC GCC^GGC
4907 GCCGGC
NgoMIV 2830 GCCGGC G^CCGGC
4907 GCCGGC
PpuMI 638 GGGTCCT [GA]G^G[AT]CC[CT]
4567 GGGTCCT
SmaI 3483 CCCGGG CCC^GGG
4589 CCCGGG
SphI 459 GCATGC GCATG^C
1336 GCATGC
XcmI 4855 CCACACACCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5423 CCACTGTTACTGTGG
XmaCI 3483 CCCGGG C^CCGGG
4589 CCCGGG
XmaI 3483 CCCGGG C^CCGGG
4589 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI