##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BclI
BfrI BglII BlnI BsaWI BseAI
BsiWI BspDI BspEI BspHI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EcoRV
FbaI FseI FspI HindIII HpaI
MfeI MluI MroI MunI NdeI
NheI NruI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I RcaI
RsrII SalI SapI SfiI SfuI
SgrAI SnaBI SpeI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1293 GGTACC G^GTACC
AocI 3639 CCTGAGG CC^T[ACGT]AGG
Asp700I 2363 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1293 GGTACC G^GTACC
BbrPI 509 CACGTG CAC^GTG
BbvCI 1892 GCTGAGG CCTCAGC(-5/-2)
BcgI 425 GCAGGCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 509 CACGTG [CT]AC^GT[GA]
BspLU11I 1855 ACATGT A^CATGT
BssHII 3525 GCGCGC G^CGCGC
BstEII 2567 GGTCACC G^GT[ACGT]ACC
BstPI 2567 GGTCACC G^GT[ACGT]ACC
Bsu36I 3639 CCTGAGG CC^T[ACGT]AGG
Eco81I 3639 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2567 GGTCACC G^GT[ACGT]ACC
EcoT22I 2826 ATGCAT ATGCA^T
HincII 903 GTCAAC GT[CT]^[GA]AC
HindII 903 GTCAAC GT[CT]^[GA]AC
KpnI 1293 GGTACC GGTAC^C
NotI 816 GCGGCCGC GC^GGCCGC
NsiI 2826 ATGCAT ATGCA^T
PaeR7I 62 CTCGAG C^TCGAG
PmaCI 509 CACGTG CAC^GTG
PmlI 509 CACGTG CAC^GTG
PshAI 3829 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 745 CGATCG CGAT^CG
SbfI 1712 CCTGCAGG CCTGCA^GG
ScaI 1147 AGTACT AGT^ACT
SexAI 4540 ACCAGGT A^CC[AT]GGT
Sse8387I 1712 CCTGCAGG CCTGCA^GG
XhoI 62 CTCGAG C^TCGAG
XmnI 2363 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1302 GTAGAC GT^[AC][GT]AC
1482 GTCTAC
AcsI 3411 GAATTC [GA]^AATT[CT]
4314 GAATTC
AflIII 1855 ACATGT A^C[GA][CT]GT
2629 ACGTGT
Alw44I 2643 GTGCAC G^TGCAC
4832 GTGCAC
Aor51HI 2605 AGCGCT AGC^GCT
4587 AGCGCT
ApaLI 2643 GTGCAC G^TGCAC
4832 GTGCAC
ApoI 3411 GAATTC [GA]^AATT[CT]
4314 GAATTC
BciVI 321 GTATCC GTATCC(6/5)
2752 GTATCC
BlpI 2313 GCTCAGC GC^T[ACGT]AGC
4490 GCTGAGC
Bpu1102I 2313 GCTCAGC GC^T[ACGT]AGC
4490 GCTGAGC
BsaBI 746 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1787 GATCGGCATC
Bsp1407I 730 TGTACA T^GTACA
3876 TGTACA
BsrGI 730 TGTACA T^GTACA
3876 TGTACA
CelII 2313 GCTCAGC GC^T[ACGT]AGC
4490 GCTGAGC
Eco47III 2605 AGCGCT AGC^GCT
4587 AGCGCT
EcoRI 3411 GAATTC G^AATTC
4314 GAATTC
KspI 3458 CCGCGG CCGC^GG
3536 CCGCGG
MamI 746 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1787 GATCGGCATC
SacI 59 GAGCTC GAGCT^C
3816 GAGCTC
SacII 3458 CCGCGG CCGC^GG
3536 CCGCGG
SspBI 730 TGTACA T^GTACA
3876 TGTACA
SspI 5286 AATATT AAT^ATT
5381 AATATT
TfiI 2080 GAATC G^A[AT]TC
2981 GAATC
XcmI 1178 CCAGGGCATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5203 CCAGACTCTGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SphI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI