################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BclI BfrI BglII BlnI BsaWI BseAI BsiWI BspDI BspEI BspHI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EcoRV FbaI FseI FspI HindIII HpaI MfeI MluI MroI MunI NdeI NheI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I RcaI RsrII SalI SapI SfiI SfuI SgrAI SnaBI SpeI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 1293 GGTACC G^GTACC AocI 3639 CCTGAGG CC^T[ACGT]AGG Asp700I 2363 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1293 GGTACC G^GTACC BbrPI 509 CACGTG CAC^GTG BbvCI 1892 GCTGAGG CCTCAGC(-5/-2) BcgI 425 GCAGGCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 509 CACGTG [CT]AC^GT[GA] BspLU11I 1855 ACATGT A^CATGT BssHII 3525 GCGCGC G^CGCGC BstEII 2567 GGTCACC G^GT[ACGT]ACC BstPI 2567 GGTCACC G^GT[ACGT]ACC Bsu36I 3639 CCTGAGG CC^T[ACGT]AGG Eco81I 3639 CCTGAGG CC^T[ACGT]AGG EcoO65I 2567 GGTCACC G^GT[ACGT]ACC EcoT22I 2826 ATGCAT ATGCA^T HincII 903 GTCAAC GT[CT]^[GA]AC HindII 903 GTCAAC GT[CT]^[GA]AC KpnI 1293 GGTACC GGTAC^C NotI 816 GCGGCCGC GC^GGCCGC NsiI 2826 ATGCAT ATGCA^T PaeR7I 62 CTCGAG C^TCGAG PmaCI 509 CACGTG CAC^GTG PmlI 509 CACGTG CAC^GTG PshAI 3829 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 745 CGATCG CGAT^CG SbfI 1712 CCTGCAGG CCTGCA^GG ScaI 1147 AGTACT AGT^ACT SexAI 4540 ACCAGGT A^CC[AT]GGT Sse8387I 1712 CCTGCAGG CCTGCA^GG XhoI 62 CTCGAG C^TCGAG XmnI 2363 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 1302 GTAGAC GT^[AC][GT]AC 1482 GTCTAC AcsI 3411 GAATTC [GA]^AATT[CT] 4314 GAATTC AflIII 1855 ACATGT A^C[GA][CT]GT 2629 ACGTGT Alw44I 2643 GTGCAC G^TGCAC 4832 GTGCAC Aor51HI 2605 AGCGCT AGC^GCT 4587 AGCGCT ApaLI 2643 GTGCAC G^TGCAC 4832 GTGCAC ApoI 3411 GAATTC [GA]^AATT[CT] 4314 GAATTC BciVI 321 GTATCC GTATCC(6/5) 2752 GTATCC BlpI 2313 GCTCAGC GC^T[ACGT]AGC 4490 GCTGAGC Bpu1102I 2313 GCTCAGC GC^T[ACGT]AGC 4490 GCTGAGC BsaBI 746 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1787 GATCGGCATC Bsp1407I 730 TGTACA T^GTACA 3876 TGTACA BsrGI 730 TGTACA T^GTACA 3876 TGTACA CelII 2313 GCTCAGC GC^T[ACGT]AGC 4490 GCTGAGC Eco47III 2605 AGCGCT AGC^GCT 4587 AGCGCT EcoRI 3411 GAATTC G^AATTC 4314 GAATTC KspI 3458 CCGCGG CCGC^GG 3536 CCGCGG MamI 746 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1787 GATCGGCATC SacI 59 GAGCTC GAGCT^C 3816 GAGCTC SacII 3458 CCGCGG CCGC^GG 3536 CCGCGG SspBI 730 TGTACA T^GTACA 3876 TGTACA SspI 5286 AATATT AAT^ATT 5381 AATATT TfiI 2080 GAATC G^A[AT]TC 2981 GAATC XcmI 1178 CCAGGGCATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5203 CCAGACTCTGGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SphI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI