##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI ApaI AscI Asp718I AspEI
AspI AviII AvrII BanI BanII
BbeI BbvCI BcnI BfrI BglI
BlnI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstPI BstUI
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I FauI FseI FspI HpaI
KasI KpnI KspI MfeI MluI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmeI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2501 GACGTC GACGT^C
AccIII 2133 TCCGGA T^CCGGA
AcyI 2501 GACGTC G[GA]^CG[CT]C
AlwNI 4121 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 361 CCTCAGG CC^T[ACGT]AGG
AseI 4357 ATTAAT AT^TAAT
AsnI 4357 ATTAAT AT^TAAT
Asp700I 4374 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1939 TGGCCA TGG^CCA
BbrPI 2264 CACGTG CAC^GTG
BcgI 1337 GCAAGAATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3504 GTATCC GTATCC(6/5)
BglII 760 AGATCT A^GATCT
BsaHI 2501 GACGTC G[GA]^CG[CT]C
BsaWI 2133 TCCGGA [AT]^CCGG[AT]
BseAI 2133 TCCGGA T^CCGGA
BspEI 2133 TCCGGA T^CCGGA
Bst1107I 3773 GTATAC GTA^TAC
BstAPI 987 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3773 GTATAC GTA^TAC
Bsu36I 361 CCTCAGG CC^T[ACGT]AGG
DraII 3919 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 361 CCTCAGG CC^T[ACGT]AGG
EcoO109I 3919 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2650 GAATTC G^AATTC
EcoRV 214 GATATC GAT^ATC
EcoT22I 2920 ATGCAT ATGCA^T
Hin1I 2501 GACGTC G[GA]^CG[CT]C
MluNI 1939 TGGCCA TGG^CCA
MroI 2133 TCCGGA T^CCGGA
MscI 1939 TGGCCA TGG^CCA
NheI 3630 GCTAGC G^CTAGC
NsiI 2920 ATGCAT ATGCA^T
PaeR7I 1748 CTCGAG C^TCGAG
PmaCI 2264 CACGTG CAC^GTG
PmlI 2264 CACGTG CAC^GTG
PpuMI 3919 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2599 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4357 ATTAAT AT^TAAT
StuI 3611 AGGCCT AGG^CCT
XhoI 1748 CTCGAG C^TCGAG
XmnI 4374 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 3135 GTGCAC G^TGCAC
3260 GTGCAC
Aor51HI 12 AGCGCT AGC^GCT
1873 AGCGCT
ApaLI 3135 GTGCAC G^TGCAC
3260 GTGCAC
BamHI 1838 GGATCC G^GATCC
4250 GGATCC
BsaAI 2264 CACGTG [CT]AC^GT[GA]
2845 CACGTA
BsaBI 945 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2277 GATCAAAATC
BsaI 522 GAGACC GGTCTC(1/5)
1231 GAGACC
BseRI 3273 CTCCTC GAGGAG(10/8)
1182 GAGGAG
BssSI 1131 CACGAG CACGAG(-5/-1)
3201 CACGAG
DraI 2349 TTTAAA TTT^AAA
4410 TTTAAA
DsaI 1040 CCATGG C^C[GA][CT]GG
2485 CCATGG
EaeI 1939 TGGCCA [CT]^GGCC[GA]
2015 CGGCCA
Eco47III 12 AGCGCT AGC^GCT
1873 AGCGCT
HapII 1704 CCGG C^CGG
2134 CCGG
HgaI 122 GACGC GACGC(5/10)
2181 GACGC
HincII 2480 GTTGAC GT[CT]^[GA]AC
3831 GTTGAC
HindII 2480 GTTGAC GT[CT]^[GA]AC
3831 GTTGAC
HindIII 296 AAGCTT A^AGCTT
1708 AAGCTT
HpaII 1704 CCGG C^CGG
2134 CCGG
MamI 945 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2277 GATCAAAATC
MspI 1704 CCGG C^CGG
2134 CCGG
NcoI 1040 CCATGG C^CATGG
2485 CCATGG
PflMI 1883 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2260 CCATCACGTGG
SapI 822 GAAGAGC GCTCTTC(1/4)
1869 GAAGAGC
SpeI 1020 ACTAGT A^CTAGT
3835 ACTAGT
SphI 2660 GCATGC GCATG^C
3538 GCATGC
Van91I 1883 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2260 CCATCACGTGG
XbaI 1994 TCTAGA T^CTAGA
3936 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BbsI BbvI BclI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII