################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccII AclI AflII AgeI AhdI ApaI AscI Asp718I AspEI AspI AviII AvrII BanI BanII BbeI BbvCI BcnI BfrI BglI BlnI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I FauI FseI FspI HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NspV PacI PflFI PinAI PmeI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI Tth111I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 2501 GACGTC GACGT^C AccIII 2133 TCCGGA T^CCGGA AcyI 2501 GACGTC G[GA]^CG[CT]C AlwNI 4121 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 361 CCTCAGG CC^T[ACGT]AGG AseI 4357 ATTAAT AT^TAAT AsnI 4357 ATTAAT AT^TAAT Asp700I 4374 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1939 TGGCCA TGG^CCA BbrPI 2264 CACGTG CAC^GTG BcgI 1337 GCAAGAATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3504 GTATCC GTATCC(6/5) BglII 760 AGATCT A^GATCT BsaHI 2501 GACGTC G[GA]^CG[CT]C BsaWI 2133 TCCGGA [AT]^CCGG[AT] BseAI 2133 TCCGGA T^CCGGA BspEI 2133 TCCGGA T^CCGGA Bst1107I 3773 GTATAC GTA^TAC BstAPI 987 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 3773 GTATAC GTA^TAC Bsu36I 361 CCTCAGG CC^T[ACGT]AGG DraII 3919 GGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 361 CCTCAGG CC^T[ACGT]AGG EcoO109I 3919 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 2650 GAATTC G^AATTC EcoRV 214 GATATC GAT^ATC EcoT22I 2920 ATGCAT ATGCA^T Hin1I 2501 GACGTC G[GA]^CG[CT]C MluNI 1939 TGGCCA TGG^CCA MroI 2133 TCCGGA T^CCGGA MscI 1939 TGGCCA TGG^CCA NheI 3630 GCTAGC G^CTAGC NsiI 2920 ATGCAT ATGCA^T PaeR7I 1748 CTCGAG C^TCGAG PmaCI 2264 CACGTG CAC^GTG PmlI 2264 CACGTG CAC^GTG PpuMI 3919 GGGACCT [GA]G^G[AT]CC[CT] PshAI 2599 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4357 ATTAAT AT^TAAT StuI 3611 AGGCCT AGG^CCT XhoI 1748 CTCGAG C^TCGAG XmnI 4374 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Alw44I 3135 GTGCAC G^TGCAC 3260 GTGCAC Aor51HI 12 AGCGCT AGC^GCT 1873 AGCGCT ApaLI 3135 GTGCAC G^TGCAC 3260 GTGCAC BamHI 1838 GGATCC G^GATCC 4250 GGATCC BsaAI 2264 CACGTG [CT]AC^GT[GA] 2845 CACGTA BsaBI 945 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2277 GATCAAAATC BsaI 522 GAGACC GGTCTC(1/5) 1231 GAGACC BseRI 3273 CTCCTC GAGGAG(10/8) 1182 GAGGAG BssSI 1131 CACGAG CACGAG(-5/-1) 3201 CACGAG DraI 2349 TTTAAA TTT^AAA 4410 TTTAAA DsaI 1040 CCATGG C^C[GA][CT]GG 2485 CCATGG EaeI 1939 TGGCCA [CT]^GGCC[GA] 2015 CGGCCA Eco47III 12 AGCGCT AGC^GCT 1873 AGCGCT HapII 1704 CCGG C^CGG 2134 CCGG HgaI 122 GACGC GACGC(5/10) 2181 GACGC HincII 2480 GTTGAC GT[CT]^[GA]AC 3831 GTTGAC HindII 2480 GTTGAC GT[CT]^[GA]AC 3831 GTTGAC HindIII 296 AAGCTT A^AGCTT 1708 AAGCTT HpaII 1704 CCGG C^CGG 2134 CCGG MamI 945 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2277 GATCAAAATC MspI 1704 CCGG C^CGG 2134 CCGG NcoI 1040 CCATGG C^CATGG 2485 CCATGG PflMI 1883 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2260 CCATCACGTGG SapI 822 GAAGAGC GCTCTTC(1/4) 1869 GAAGAGC SpeI 1020 ACTAGT A^CTAGT 3835 ACTAGT SphI 2660 GCATGC GCATG^C 3538 GCATGC Van91I 1883 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2260 CCATCACGTGG XbaI 1994 TCTAGA T^CTAGA 3936 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaI AvaII BbsI BbvI BclI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII