##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
BfrI BsaBI BsiWI BssHII Bst1107I
BstBI BstZ17I CpoI DrdI Eam1105I
EcoT22I FseI KspI MamI MfeI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SfiI SfuI SgrAI SnaBI SphI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 836 GACGTC GACGT^C
Acc65I 1923 GGTACC G^GTACC
AccIII 1673 TCCGGA T^CCGGA
Aor51HI 118 AGCGCT AGC^GCT
Asp700I 2442 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1923 GGTACC G^GTACC
AviII 5683 TGCGCA TGC^GCA
AvrII 5494 CCTAGG C^CTAGG
BamHI 1598 GGATCC G^GATCC
BbrPI 4739 CACGTG CAC^GTG
BclI 5267 TGATCA T^GATCA
BglII 3965 AGATCT A^GATCT
BlnI 5494 CCTAGG C^CTAGG
BseAI 1673 TCCGGA T^CCGGA
BsmI 2584 GAATGC GAATGC(1/-1)
BspDI 3752 ATCGAT AT^CGAT
BspEI 1673 TCCGGA T^CCGGA
BspLU11I 5149 ACATGT A^CATGT
BsrDI 1641 GCAATG GCAATG(2/0)
ClaI 3752 ATCGAT AT^CGAT
EagI 2241 CGGCCG C^GGCCG
EclXI 2241 CGGCCG C^GGCCG
Eco47III 118 AGCGCT AGC^GCT
Eco52I 2241 CGGCCG C^GGCCG
EcoRI 2783 GAATTC G^AATTC
EcoRV 1405 GATATC GAT^ATC
FbaI 5267 TGATCA T^GATCA
FspI 5683 TGCGCA TGC^GCA
HindIII 872 AAGCTT A^AGCTT
HpaI 5471 GTTAAC GTT^AAC
KpnI 1923 GGTACC GGTAC^C
MluI 2506 ACGCGT A^CGCGT
MroI 1673 TCCGGA T^CCGGA
NcoI 654 CCATGG C^CATGG
NdeI 3810 CATATG CA^TATG
PmaCI 4739 CACGTG CAC^GTG
PmlI 4739 CACGTG CAC^GTG
SalI 3933 GTCGAC G^TCGAC
SapI 3058 GCTCTTC GCTCTTC(1/4)
SbfI 2374 CCTGCAGG CCTGCA^GG
ScaI 591 AGTACT AGT^ACT
SexAI 1014 ACCTGGT A^CC[AT]GGT
SmlI 4156 CTTGAG C^T[CT][GA]AG
SpeI 4538 ACTAGT A^CTAGT
Sse8387I 2374 CCTGCAGG CCTGCA^GG
SspI 5197 AATATT AAT^ATT
XbaI 3341 TCTAGA T^CTAGA
XmnI 2442 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 3933 GTCGAC GT^[AC][GT]AC
5092 GTAGAC
ApaI 2219 GGGCCC GGGCC^C
4334 GGGCCC
AspI 1910 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4731 GACACCGTC
BbeI 2719 GGCGCC GGCGC^C
3115 GGCGCC
BbvCI 200 CCTCAGC CCTCAGC(-5/-2)
2251 CCTCAGC
BcgI 5026 CGACATGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2148 GCAGCACCATCG
BlpI 3330 GCTCAGC GC^T[ACGT]AGC
4856 GCTCAGC
Bpu1102I 3330 GCTCAGC GC^T[ACGT]AGC
4856 GCTCAGC
BsaI 4386 GAGACC GGTCTC(1/5)
4905 GGTCTC
BsaWI 1662 ACCGGA [AT]^CCGG[AT]
1673 TCCGGA
BsiEI 1252 CGACCG CG[GA][CT]^CG
2241 CGGCCG
BsmBI 1220 GAGACG CGTCTC(1/5)
2195 GAGACG
Bsp1407I 747 TGTACA T^GTACA
4598 TGTACA
BsrGI 747 TGTACA T^GTACA
4598 TGTACA
CelII 3330 GCTCAGC GC^T[ACGT]AGC
4856 GCTCAGC
KasI 2719 GGCGCC G^GCGCC
3115 GGCGCC
NaeI 2243 GCCGGC GCC^GGC
3023 GCCGGC
NarI 2719 GGCGCC GG^CGCC
3115 GGCGCC
NgoMIV 2243 GCCGGC G^CCGGC
3023 GCCGGC
PflFI 1910 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4731 GACACCGTC
SfoI 2719 GGCGCC GGC^GCC
3115 GGCGCC
SspBI 747 TGTACA T^GTACA
4598 TGTACA
StuI 2886 AGGCCT AGG^CCT
5190 AGGCCT
Tth111I 1910 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4731 GACACCGTC
XcmI 2154 CCATCGTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2292 CCAAGGAAGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI