##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccII AflII AgeI AhdI AocI
Aor51HI ApaI AscI AspEI AviII
BbeI BbrPI BbvCI BfrI BglI
BglII BsaAI BsiEI BsiWI BsmBI
Bsp1407I BsrGI BssHII BstBI BstUI
Bsu36I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FseI FspI HaeII HgaI
HindIII KasI KspI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmlI
PshAI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 378 GACGTC GACGT^C
AccIII 3602 TCCGGA T^CCGGA
AclI 604 AACGTT AA^CGTT
AcyI 378 GACGTC G[GA]^CG[CT]C
Alw44I 4637 GTGCAC G^TGCAC
ApaLI 4637 GTGCAC G^TGCAC
Asp700I 4573 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4446 CCTAGG C^CTAGG
BalI 857 TGGCCA TGG^CCA
BcgI 971 GCAGTAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 812 TGATCA T^GATCA
BlnI 4446 CCTAGG C^CTAGG
BsaBI 2953 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 378 GACGTC G[GA]^CG[CT]C
BsaI 2348 GGTCTC GGTCTC(1/5)
BseAI 3602 TCCGGA T^CCGGA
BspDI 1164 ATCGAT AT^CGAT
BspEI 3602 TCCGGA T^CCGGA
BsrFI 4114 GCCGGT [GA]^CCGG[CT]
BssSI 1360 CTCGTG CACGAG(-5/-1)
Bst1107I 1775 GTATAC GTA^TAC
BstEII 904 GGTAACC G^GT[ACGT]ACC
BstPI 904 GGTAACC G^GT[ACGT]ACC
BstXI 839 CCAGGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1775 GTATAC GTA^TAC
Cfr10I 4114 GCCGGT [GA]^CCGG[CT]
ClaI 1164 ATCGAT AT^CGAT
DrdI 211 GACAAAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 857 TGGCCA [CT]^GGCC[GA]
EcoO65I 904 GGTAACC G^GT[ACGT]ACC
FauI 2812 CCCGC CCCGC(4/6)
FbaI 812 TGATCA T^GATCA
Hin1I 378 GACGTC G[GA]^CG[CT]C
HpaI 4231 GTTAAC GTT^AAC
MamI 2953 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 857 TGGCCA TGG^CCA
MroI 3602 TCCGGA T^CCGGA
MscI 857 TGGCCA TGG^CCA
PflMI 3790 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 498 GTTTAAAC GTTT^AAAC
Psp1406I 604 AACGTT AA^CGTT
ScaI 191 AGTACT AGT^ACT
SphI 773 GCATGC GCATG^C
Van91I 3790 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 4573 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3040 GGTACC G^GTACC
3757 GGTACC
AseI 4502 ATTAAT AT^TAAT
4567 ATTAAT
AsnI 4502 ATTAAT AT^TAAT
4567 ATTAAT
Asp718I 3040 GGTACC G^GTACC
3757 GGTACC
AspHI 4118 GTGCTC G[AT]GC[AT]^C
4637 GTGCAC
AspI 4078 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4399 GACTTAGTC
AvaI 4121 CTCGGG C^[CT]CG[GA]G
4659 CTCGGG
BamHI 2970 GGATCC G^GATCC
3118 GGATCC
BanII 2391 GAGCCC G[GA]GC[CT]^C
2663 GGGCTC
BpmI 4373 CTCCAG CTGGAG(16/14)
3985 CTGGAG
BsaWI 2721 TCCGGT [AT]^CCGG[AT]
3602 TCCGGA
BseRI 2870 CTCCTC GAGGAG(10/8)
2636 GAGGAG
BsiHKAI 4118 GTGCTC G[AT]GC[AT]^C
4637 GTGCAC
BsmI 2746 GAATGC GAATGC(1/-1)
260 GCATTC
BsoBI 4121 CTCGGG C^[CT]CG[GA]G
4659 CTCGGG
BspHI 3341 TCATGA T^CATGA
3638 TCATGA
BspLU11I 1958 ACATGT A^CATGT
3862 ACATGT
BsrBI 166 CCGCTC CCGCTC(-3/-3)
2813 CCGCTC
CfoI 2331 GCGC GCG^C
4277 GCGC
DsaI 2477 CCATGG C^C[GA][CT]GG
4469 CCATGG
EcoNI 19 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2184 CCTACTAAAGG
EcoRI 2092 GAATTC G^AATTC
3683 GAATTC
HhaI 2331 GCGC GCG^C
4277 GCGC
HinP1I 2331 GCGC G^CGC
4277 GCGC
KpnI 3040 GGTACC GGTAC^C
3757 GGTACC
NcoI 2477 CCATGG C^CATGG
4469 CCATGG
NheI 3559 GCTAGC G^CTAGC
3576 GCTAGC
PflFI 4078 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4399 GACTTAGTC
PpuMI 520 GGGACCC [GA]G^G[AT]CC[CT]
3945 AGGACCT
PshBI 4502 ATTAAT AT^TAAT
4567 ATTAAT
RcaI 3341 TCATGA T^CATGA
3638 TCATGA
SspI 1760 AATATT AAT^ATT
4499 AATATT
Tth111I 4078 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4399 GACTTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AvaII
BanI BbsI BbvI BciVI BcnI
BfaI BlpI BmyI Bpu1102I BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII