##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
AseI AsnI AspI AviII AvrII
BamHI BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI BspEI
BspHI BssHII Bst1107I BstBI BstZ17I
CelII CpoI DraI EagI EclXI
Eco52I EcoRI EcoRV FseI FspI
HpaI KspI MamI MluI MroI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SspI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 93 CGCG CG^CG
Alw44I 4508 GTGCAC G^TGCAC
Aor51HI 2192 AGCGCT AGC^GCT
ApaLI 4508 GTGCAC G^TGCAC
BbrPI 5081 CACGTG CAC^GTG
BciVI 4043 GGATAC GTATCC(6/5)
BglI 4322 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1206 AGATCT A^GATCT
Bsp1407I 1544 TGTACA T^GTACA
BspDI 1963 ATCGAT AT^CGAT
BsrGI 1544 TGTACA T^GTACA
BssSI 843 CTCGTG CACGAG(-5/-1)
BstUI 93 CGCG CG^CG
ClaI 1963 ATCGAT AT^CGAT
DrdI 2914 GACATGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2192 AGCGCT AGC^GCT
HindIII 114 AAGCTT A^AGCTT
MfeI 650 CAATTG C^AATTG
MunI 650 CAATTG C^AATTG
MvnI 93 CGCG CG^CG
NaeI 60 GCCGGC GCC^GGC
NdeI 1454 CATATG CA^TATG
NgoMIV 60 GCCGGC G^CCGGC
PaeR7I 180 CTCGAG C^TCGAG
PmaCI 5081 CACGTG CAC^GTG
PmlI 5081 CACGTG CAC^GTG
SbfI 3769 CCTGCAGG CCTGCA^GG
SmaI 4443 CCCGGG CCC^GGG
Sse8387I 3769 CCTGCAGG CCTGCA^GG
SspBI 1544 TGTACA T^GTACA
XbaI 1611 TCTAGA T^CTAGA
XhoI 180 CTCGAG C^TCGAG
XmaCI 4443 CCCGGG C^CCGGG
XmaI 4443 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 489 GACGTC GACGT^C
3204 GACGTC
Acc65I 499 GGTACC G^GTACC
897 GGTACC
AhdI 177 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1626 GACTAGAAGTC
AocI 4650 CCTCAGG CC^T[ACGT]AGG
4715 CCTCAGG
ApaI 1906 GGGCCC GGGCC^C
3045 GGGCCC
Asp718I 499 GGTACC G^GTACC
897 GGTACC
AspEI 177 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1626 GACTAGAAGTC
BbsI 432 GAAGAC GAAGAC(2/6)
3776 GAAGAC
BcgI 1911 CGAGCCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
283 GCAACTCCATCG
BpuAI 432 GAAGAC GAAGAC(2/6)
3776 GAAGAC
BsaAI 5081 CACGTG [CT]AC^GT[GA]
5098 CACGTA
BsrBI 825 CCGCTC CCGCTC(-3/-3)
2560 GAGCGG
BstAPI 1588 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2844 GCAGACTATGC
BstEII 464 GGTTACC G^GT[ACGT]ACC
5378 GGTTACC
BstPI 464 GGTTACC G^GT[ACGT]ACC
5378 GGTTACC
BstYI 1206 AGATCT [GA]^GATC[CT]
2255 GGATCT
Bsu36I 4650 CCTCAGG CC^T[ACGT]AGG
4715 CCTCAGG
DraIII 2080 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5333 CACAGTGTG
Eam1105I 177 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1626 GACTAGAAGTC
Eco81I 4650 CCTCAGG CC^T[ACGT]AGG
4715 CCTCAGG
EcoO65I 464 GGTTACC G^GT[ACGT]ACC
5378 GGTTACC
EcoT22I 3841 ATGCAT ATGCA^T
5365 ATGCAT
HgaI 2140 GCGTC GACGC(5/10)
3096 GCGTC
KpnI 499 GGTACC GGTAC^C
897 GGTACC
MflI 1206 AGATCT [GA]^GATC[CT]
2255 GGATCT
NsiI 3841 ATGCAT ATGCA^T
5365 ATGCAT
XhoII 1206 AGATCT [GA]^GATC[CT]
2255 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BclI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XmnI