##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AccIII AclI Alw44I ApaLI AscI
AseI AsnI BbrPI BclI BsaAI
BseAI BsiWI BsmI BspEI BspHI
BssHII Bst1107I BstZ17I CpoI DraIII
EagI EclXI Eco52I EcoRI FbaI
FseI MluI MroI NaeI NdeI
NgoMIV NotI NruI PacI PmaCI
PmeI PmlI PshBI Psp1406I RcaI
RsrII SbfI ScaI SfiI SgrAI
SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3012 GACGTC GACGT^C
Acc65I 242 GGTACC G^GTACC
AflII 4766 CTTAAG C^TTAAG
AgeI 4511 ACCGGT A^CCGGT
AhdI 3292 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 242 GGTACC G^GTACC
AspEI 3292 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 255 TGCGCA TGC^GCA
BamHI 4786 GGATCC G^GATCC
BbeI 2 GGCGCC GGCGC^C
BciVI 5331 GTATCC GTATCC(6/5)
BfrI 4766 CTTAAG C^TTAAG
BglI 217 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 4511 ACCGGT [AT]^CCGG[AT]
Bsp1407I 6015 TGTACA T^GTACA
BspDI 596 ATCGAT AT^CGAT
BspLU11I 5623 ACATGT A^CATGT
BsrGI 6015 TGTACA T^GTACA
BstBI 2186 TTCGAA TT^CGAA
BstEII 1550 GGTTACC G^GT[ACGT]ACC
BstPI 1550 GGTTACC G^GT[ACGT]ACC
ClaI 596 ATCGAT AT^CGAT
DraI 4504 TTTAAA TTT^AAA
Eam1105I 3292 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1550 GGTTACC G^GT[ACGT]ACC
EcoRV 4634 GATATC GAT^ATC
EcoT22I 4117 ATGCAT ATGCA^T
FspI 255 TGCGCA TGC^GCA
HpaI 2550 GTTAAC GTT^AAC
KasI 2 GGCGCC G^GCGCC
KpnI 242 GGTACC GGTAC^C
KspI 117 CCGCGG CCGC^GG
MfeI 2644 CAATTG C^AATTG
MunI 2644 CAATTG C^AATTG
NarI 2 GGCGCC GG^CGCC
NheI 1278 GCTAGC G^CTAGC
NsiI 4117 ATGCAT ATGCA^T
NspV 2186 TTCGAA TT^CGAA
PinAI 4511 ACCGGT A^CCGGT
PshAI 3875 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 117 CCGCGG CCGC^GG
SalI 3361 GTCGAC G^TCGAC
SfoI 2 GGCGCC GGC^GCC
SfuI 2186 TTCGAA TT^CGAA
SpeI 3501 ACTAGT A^CTAGT
SspBI 6015 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 5623 ACATGT A^C[GA][CT]GT
6052 ACACGT
AocI 2520 CCTGAGG CC^T[ACGT]AGG
4473 CCTCAGG
AspI 2717 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2970 GACATCGTC
AvrII 519 CCTAGG C^CTAGG
4456 CCTAGG
BcgI 146 GCAGCGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
181 GCATCCCAATCG
BglII 3330 AGATCT A^GATCT
4948 AGATCT
BlnI 519 CCTAGG C^CTAGG
4456 CCTAGG
BlpI 1903 GCTCAGC GC^T[ACGT]AGC
4305 GCTGAGC
Bpu1102I 1903 GCTCAGC GC^T[ACGT]AGC
4305 GCTGAGC
BsaBI 2826 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5326 GATGTGTATC
BsmBI 2975 CGTCTC CGTCTC(1/5)
3602 GAGACG
BsrBI 375 CCGCTC CCGCTC(-3/-3)
2365 GAGCGG
BssSI 2821 CACGAG CACGAG(-5/-1)
536 CTCGTG
BstAPI 2931 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3477 GCAGTTGCTGC
Bsu36I 2520 CCTGAGG CC^T[ACGT]AGG
4473 CCTCAGG
CelII 1903 GCTCAGC GC^T[ACGT]AGC
4305 GCTGAGC
Eco81I 2520 CCTGAGG CC^T[ACGT]AGG
4473 CCTCAGG
MamI 2826 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5326 GATGTGTATC
PaeR7I 3071 CTCGAG C^TCGAG
3190 CTCGAG
PflFI 2717 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2970 GACATCGTC
SexAI 1122 ACCAGGT A^CC[AT]GGT
1802 ACCAGGT
SmaI 32 CCCGGG CCC^GGG
437 CCCGGG
SphI 3756 GCATGC GCATG^C
6083 GCATGC
Tth111I 2717 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2970 GACATCGTC
XhoI 3071 CTCGAG C^TCGAG
3190 CTCGAG
XmaCI 32 CCCGGG C^CCGGG
437 CCCGGG
XmaI 32 CCCGGG C^CCGGG
437 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI Aor51HI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco47III EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII XmnI