################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AclI AgeI AhdI Aor51HI AscI AspEI AspI AvrII BbrPI BciVI BlnI BsaAI BsgI BsiEI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FseI HindIII HpaI MluI NdeI NotI NsiI NspV PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SalI SapI SexAI SfiI SfuI SnaBI SpeI SspBI SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1918 GACGTC GACGT^C Acc65I 1231 GGTACC G^GTACC AccI 2006 GTAGAC GT^[AC][GT]AC AflII 2625 CTTAAG C^TTAAG AflIII 3779 ACACGT A^C[GA][CT]GT AocI 1108 CCTCAGG CC^T[ACGT]AGG AseI 3108 ATTAAT AT^TAAT AsnI 3108 ATTAAT AT^TAAT Asp718I 1231 GGTACC G^GTACC AviII 3888 TGCGCA TGC^GCA BbeI 213 GGCGCC GGCGC^C BcgI 3050 GCACTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2625 CTTAAG C^TTAAG BglII 122 AGATCT A^GATCT BsaBI 117 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 592 CGTACG C^GTACG BsmBI 862 CGTCTC CGTCTC(1/5) BssHII 236 GCGCGC G^CGCGC BstEII 4498 GGTGACC G^GT[ACGT]ACC BstPI 4498 GGTGACC G^GT[ACGT]ACC Bsu36I 1108 CCTCAGG CC^T[ACGT]AGG Eco81I 1108 CCTCAGG CC^T[ACGT]AGG EcoO65I 4498 GGTGACC G^GT[ACGT]ACC EcoRI 1737 GAATTC G^AATTC FspI 3888 TGCGCA TGC^GCA KasI 213 GGCGCC G^GCGCC KpnI 1231 GGTACC GGTAC^C KspI 331 CCGCGG CCGC^GG MamI 117 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2835 CAATTG C^AATTG MunI 2835 CAATTG C^AATTG NaeI 587 GCCGGC GCC^GGC NarI 213 GGCGCC GG^CGCC NgoMIV 587 GCCGGC G^CCGGC NheI 1517 GCTAGC G^CTAGC NspI 1702 GCATGC [GA]CATG^[CT] PacI 4907 TTAATTAA TTAAT^TAA PaeR7I 146 CTCGAG C^TCGAG PshAI 2241 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3108 ATTAAT AT^TAAT SacII 331 CCGCGG CCGC^GG SbfI 681 CCTGCAGG CCTGCA^GG ScaI 4393 AGTACT AGT^ACT SfoI 213 GGCGCC GGC^GCC SgrAI 104 CGCCGGTG C[GA]^CCGG[CT]G SphI 1702 GCATGC GCATG^C Sse8387I 681 CCTGCAGG CCTGCA^GG XhoI 146 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 1737 GAATTC [GA]^AATT[CT] 4894 GAATTT AcyI 213 GGCGCC G[GA]^CG[CT]C 1918 GACGTC Alw44I 1695 GTGCAC G^TGCAC 1946 GTGCAC ApaLI 1695 GTGCAC G^TGCAC 1946 GTGCAC ApoI 1737 GAATTC [GA]^AATT[CT] 4894 GAATTT Asp700I 1504 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1832 GAAAGTCTTC BalI 2206 TGGCCA TGG^CCA 2333 TGGCCA BclI 408 TGATCA T^GATCA 1062 TGATCA BsaHI 213 GGCGCC G[GA]^CG[CT]C 1918 GACGTC BsaI 872 GAGACC GGTCTC(1/5) 3851 GAGACC BsrBI 3224 CCGCTC CCGCTC(-3/-3) 4231 GAGCGG BsrFI 105 GCCGGT [GA]^CCGG[CT] 587 GCCGGC BstAPI 1697 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2614 GCACCCATTGC Cfr10I 105 GCCGGT [GA]^CCGG[CT] 587 GCCGGC DraI 1788 TTTAAA TTT^AAA 4812 TTTAAA FbaI 408 TGATCA T^GATCA 1062 TGATCA Hin1I 213 GGCGCC G[GA]^CG[CT]C 1918 GACGTC MluNI 2206 TGGCCA TGG^CCA 2333 TGGCCA MscI 2206 TGGCCA TGG^CCA 2333 TGGCCA NruI 154 TCGCGA TCG^CGA 458 TCGCGA PflMI 53 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1583 CCAGGCCCTGG Van91I 53 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1583 CCAGGCCCTGG XbaI 2237 TCTAGA T^CTAGA 4455 TCTAGA XmnI 1504 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1832 GAAAGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AccIII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseAI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI