##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AgeI AhdI Aor51HI AscI
AspEI AspI AvrII BbrPI BciVI
BlnI BsaAI BsgI BsiEI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FseI
HindIII HpaI MluI NdeI NotI
NsiI NspV PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SalI SapI SexAI SfiI
SfuI SnaBI SpeI SspBI SspI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1918 GACGTC GACGT^C
Acc65I 1231 GGTACC G^GTACC
AccI 2006 GTAGAC GT^[AC][GT]AC
AflII 2625 CTTAAG C^TTAAG
AflIII 3779 ACACGT A^C[GA][CT]GT
AocI 1108 CCTCAGG CC^T[ACGT]AGG
AseI 3108 ATTAAT AT^TAAT
AsnI 3108 ATTAAT AT^TAAT
Asp718I 1231 GGTACC G^GTACC
AviII 3888 TGCGCA TGC^GCA
BbeI 213 GGCGCC GGCGC^C
BcgI 3050 GCACTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2625 CTTAAG C^TTAAG
BglII 122 AGATCT A^GATCT
BsaBI 117 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 592 CGTACG C^GTACG
BsmBI 862 CGTCTC CGTCTC(1/5)
BssHII 236 GCGCGC G^CGCGC
BstEII 4498 GGTGACC G^GT[ACGT]ACC
BstPI 4498 GGTGACC G^GT[ACGT]ACC
Bsu36I 1108 CCTCAGG CC^T[ACGT]AGG
Eco81I 1108 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4498 GGTGACC G^GT[ACGT]ACC
EcoRI 1737 GAATTC G^AATTC
FspI 3888 TGCGCA TGC^GCA
KasI 213 GGCGCC G^GCGCC
KpnI 1231 GGTACC GGTAC^C
KspI 331 CCGCGG CCGC^GG
MamI 117 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2835 CAATTG C^AATTG
MunI 2835 CAATTG C^AATTG
NaeI 587 GCCGGC GCC^GGC
NarI 213 GGCGCC GG^CGCC
NgoMIV 587 GCCGGC G^CCGGC
NheI 1517 GCTAGC G^CTAGC
NspI 1702 GCATGC [GA]CATG^[CT]
PacI 4907 TTAATTAA TTAAT^TAA
PaeR7I 146 CTCGAG C^TCGAG
PshAI 2241 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3108 ATTAAT AT^TAAT
SacII 331 CCGCGG CCGC^GG
SbfI 681 CCTGCAGG CCTGCA^GG
ScaI 4393 AGTACT AGT^ACT
SfoI 213 GGCGCC GGC^GCC
SgrAI 104 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1702 GCATGC GCATG^C
Sse8387I 681 CCTGCAGG CCTGCA^GG
XhoI 146 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1737 GAATTC [GA]^AATT[CT]
4894 GAATTT
AcyI 213 GGCGCC G[GA]^CG[CT]C
1918 GACGTC
Alw44I 1695 GTGCAC G^TGCAC
1946 GTGCAC
ApaLI 1695 GTGCAC G^TGCAC
1946 GTGCAC
ApoI 1737 GAATTC [GA]^AATT[CT]
4894 GAATTT
Asp700I 1504 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1832 GAAAGTCTTC
BalI 2206 TGGCCA TGG^CCA
2333 TGGCCA
BclI 408 TGATCA T^GATCA
1062 TGATCA
BsaHI 213 GGCGCC G[GA]^CG[CT]C
1918 GACGTC
BsaI 872 GAGACC GGTCTC(1/5)
3851 GAGACC
BsrBI 3224 CCGCTC CCGCTC(-3/-3)
4231 GAGCGG
BsrFI 105 GCCGGT [GA]^CCGG[CT]
587 GCCGGC
BstAPI 1697 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2614 GCACCCATTGC
Cfr10I 105 GCCGGT [GA]^CCGG[CT]
587 GCCGGC
DraI 1788 TTTAAA TTT^AAA
4812 TTTAAA
FbaI 408 TGATCA T^GATCA
1062 TGATCA
Hin1I 213 GGCGCC G[GA]^CG[CT]C
1918 GACGTC
MluNI 2206 TGGCCA TGG^CCA
2333 TGGCCA
MscI 2206 TGGCCA TGG^CCA
2333 TGGCCA
NruI 154 TCGCGA TCG^CGA
458 TCGCGA
PflMI 53 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1583 CCAGGCCCTGG
Van91I 53 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1583 CCAGGCCCTGG
XbaI 2237 TCTAGA T^CTAGA
4455 TCTAGA
XmnI 1504 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1832 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsaWI BseAI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI