##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
BciVI BclI BglI BsiWI BspHI
BsrFI BssHII BstBI BstXI Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoRV FbaI FseI KspI
MfeI MluI MunI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 107 GGTACC G^GTACC
AflIII 2584 ACATGT A^C[GA][CT]GT
AhdI 4736 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3037 CCTGAGG CC^T[ACGT]AGG
Asp718I 107 GGTACC G^GTACC
AspEI 4736 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 28 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2410 TGCGCA TGC^GCA
AvrII 2345 CCTAGG C^CTAGG
BbeI 1750 GGCGCC GGCGC^C
BcgI 4261 GCACCGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2345 CCTAGG C^CTAGG
BsaBI 3742 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 117 CGGTCG CG[GA][CT]^CG
BsmBI 151 GAGACG CGTCTC(1/5)
BspDI 729 ATCGAT AT^CGAT
BspLU11I 2584 ACATGT A^CATGT
BstEII 1817 GGTCACC G^GT[ACGT]ACC
BstPI 1817 GGTCACC G^GT[ACGT]ACC
Bsu36I 3037 CCTGAGG CC^T[ACGT]AGG
ClaI 729 ATCGAT AT^CGAT
DraI 3123 TTTAAA TTT^AAA
Eam1105I 4736 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3037 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1817 GGTCACC G^GT[ACGT]ACC
EcoRI 2796 GAATTC G^AATTC
FspI 2410 TGCGCA TGC^GCA
HindIII 1808 AAGCTT A^AGCTT
KasI 1750 GGCGCC G^GCGCC
KpnI 107 GGTACC GGTAC^C
MamI 3742 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1750 GGCGCC GG^CGCC
NheI 828 GCTAGC G^CTAGC
PaeR7I 2064 CTCGAG C^TCGAG
PflFI 28 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 388 ACCAGGT A^CC[AT]GGT
SfoI 1750 GGCGCC GGC^GCC
SpeI 4031 ACTAGT A^CTAGT
SspI 3605 AATATT AAT^ATT
Tth111I 28 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2064 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 624 AAATTC [GA]^AATT[CT]
2796 GAATTC
AcyI 657 GACGCC G[GA]^CG[CT]C
1750 GGCGCC
AflII 3722 CTTAAG C^TTAAG
5106 CTTAAG
ApoI 624 AAATTC [GA]^AATT[CT]
2796 GAATTC
BamHI 3196 GGATCC G^GATCC
5015 GGATCC
BbrPI 465 CACGTG CAC^GTG
2751 CACGTG
BfrI 3722 CTTAAG C^TTAAG
5106 CTTAAG
BsaAI 465 CACGTG [CT]AC^GT[GA]
2751 CACGTG
BsaHI 657 GACGCC G[GA]^CG[CT]C
1750 GGCGCC
BsaI 3030 GAGACC GGTCTC(1/5)
3260 GAGACC
Bsp1407I 5074 TGTACA T^GTACA
5193 TGTACA
BsrDI 1415 CATTGC GCAATG(2/0)
1475 CATTGC
BsrGI 5074 TGTACA T^GTACA
5193 TGTACA
BssSI 2246 CACGAG CACGAG(-5/-1)
2373 CACGAG
Bst1107I 3484 GTATAC GTA^TAC
3516 GTATAC
BstAPI 554 GCATAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3712 GCAGCTGGTGC
BstZ17I 3484 GTATAC GTA^TAC
3516 GTATAC
DrdI 657 GACGCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3892 GACTATGTGGTC
EcoT22I 733 ATGCAT ATGCA^T
2762 ATGCAT
HaeII 1750 GGCGCC [GA]GCGC^[CT]
2511 AGCGCC
Hin1I 657 GACGCC G[GA]^CG[CT]C
1750 GGCGCC
HpaI 3776 GTTAAC GTT^AAC
4106 GTTAAC
MslI 668 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4231 CATGATGGTG
NsiI 733 ATGCAT ATGCA^T
2762 ATGCAT
PmaCI 465 CACGTG CAC^GTG
2751 CACGTG
PmlI 465 CACGTG CAC^GTG
2751 CACGTG
PshAI 3212 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4759 GACTTCCGTC
SapI 230 GAAGAGC GCTCTTC(1/4)
1382 GAAGAGC
ScaI 2353 AGTACT AGT^ACT
2431 AGTACT
SphI 2764 GCATGC GCATG^C
4542 GCATGC
SspBI 5074 TGTACA T^GTACA
5193 TGTACA
StuI 3367 AGGCCT AGG^CCT
3428 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AccIII AciI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII