################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I BciVI BclI BglI BsiWI BspHI BsrFI BssHII BstBI BstXI Cfr10I CpoI DraIII EagI EclXI Eco47III Eco52I EcoRV FbaI FseI KspI MfeI MluI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 107 GGTACC G^GTACC AflIII 2584 ACATGT A^C[GA][CT]GT AhdI 4736 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3037 CCTGAGG CC^T[ACGT]AGG Asp718I 107 GGTACC G^GTACC AspEI 4736 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 28 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2410 TGCGCA TGC^GCA AvrII 2345 CCTAGG C^CTAGG BbeI 1750 GGCGCC GGCGC^C BcgI 4261 GCACCGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 2345 CCTAGG C^CTAGG BsaBI 3742 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 117 CGGTCG CG[GA][CT]^CG BsmBI 151 GAGACG CGTCTC(1/5) BspDI 729 ATCGAT AT^CGAT BspLU11I 2584 ACATGT A^CATGT BstEII 1817 GGTCACC G^GT[ACGT]ACC BstPI 1817 GGTCACC G^GT[ACGT]ACC Bsu36I 3037 CCTGAGG CC^T[ACGT]AGG ClaI 729 ATCGAT AT^CGAT DraI 3123 TTTAAA TTT^AAA Eam1105I 4736 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3037 CCTGAGG CC^T[ACGT]AGG EcoO65I 1817 GGTCACC G^GT[ACGT]ACC EcoRI 2796 GAATTC G^AATTC FspI 2410 TGCGCA TGC^GCA HindIII 1808 AAGCTT A^AGCTT KasI 1750 GGCGCC G^GCGCC KpnI 107 GGTACC GGTAC^C MamI 3742 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1750 GGCGCC GG^CGCC NheI 828 GCTAGC G^CTAGC PaeR7I 2064 CTCGAG C^TCGAG PflFI 28 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC SexAI 388 ACCAGGT A^CC[AT]GGT SfoI 1750 GGCGCC GGC^GCC SpeI 4031 ACTAGT A^CTAGT SspI 3605 AATATT AAT^ATT Tth111I 28 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2064 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 624 AAATTC [GA]^AATT[CT] 2796 GAATTC AcyI 657 GACGCC G[GA]^CG[CT]C 1750 GGCGCC AflII 3722 CTTAAG C^TTAAG 5106 CTTAAG ApoI 624 AAATTC [GA]^AATT[CT] 2796 GAATTC BamHI 3196 GGATCC G^GATCC 5015 GGATCC BbrPI 465 CACGTG CAC^GTG 2751 CACGTG BfrI 3722 CTTAAG C^TTAAG 5106 CTTAAG BsaAI 465 CACGTG [CT]AC^GT[GA] 2751 CACGTG BsaHI 657 GACGCC G[GA]^CG[CT]C 1750 GGCGCC BsaI 3030 GAGACC GGTCTC(1/5) 3260 GAGACC Bsp1407I 5074 TGTACA T^GTACA 5193 TGTACA BsrDI 1415 CATTGC GCAATG(2/0) 1475 CATTGC BsrGI 5074 TGTACA T^GTACA 5193 TGTACA BssSI 2246 CACGAG CACGAG(-5/-1) 2373 CACGAG Bst1107I 3484 GTATAC GTA^TAC 3516 GTATAC BstAPI 554 GCATAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3712 GCAGCTGGTGC BstZ17I 3484 GTATAC GTA^TAC 3516 GTATAC DrdI 657 GACGCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3892 GACTATGTGGTC EcoT22I 733 ATGCAT ATGCA^T 2762 ATGCAT HaeII 1750 GGCGCC [GA]GCGC^[CT] 2511 AGCGCC Hin1I 657 GACGCC G[GA]^CG[CT]C 1750 GGCGCC HpaI 3776 GTTAAC GTT^AAC 4106 GTTAAC MslI 668 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4231 CATGATGGTG NsiI 733 ATGCAT ATGCA^T 2762 ATGCAT PmaCI 465 CACGTG CAC^GTG 2751 CACGTG PmlI 465 CACGTG CAC^GTG 2751 CACGTG PshAI 3212 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4759 GACTTCCGTC SapI 230 GAAGAGC GCTCTTC(1/4) 1382 GAAGAGC ScaI 2353 AGTACT AGT^ACT 2431 AGTACT SphI 2764 GCATGC GCATG^C 4542 GCATGC SspBI 5074 TGTACA T^GTACA 5193 TGTACA StuI 3367 AGGCCT AGG^CCT 3428 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AccIII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspEI BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII