##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI AspI BfrI BsaBI BsiWI
BsmI BssHII Bst1107I BstBI BstZ17I
DraI EagI EclXI Eco52I EcoRV
EcoT22I FseI HindIII HpaI MamI
MfeI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I SfiI SfuI SnaBI SpeI
SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1571 GACGTC GACGT^C
Acc65I 624 GGTACC G^GTACC
AhdI 6658 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5631 GTGCAC G^TGCAC
ApaLI 5631 GTGCAC G^TGCAC
Asp700I 275 GAATACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 624 GGTACC G^GTACC
AspEI 6658 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3732 TGCGCA TGC^GCA
BbrPI 4459 CACGTG CAC^GTG
BciVI 1228 GGATAC GTATCC(6/5)
BclI 6507 TGATCA T^GATCA
BsaAI 4459 CACGTG [CT]AC^GT[GA]
Bsp1407I 4470 TGTACA T^GTACA
BspDI 4819 ATCGAT AT^CGAT
BsrGI 4470 TGTACA T^GTACA
ClaI 4819 ATCGAT AT^CGAT
CpoI 2706 CGGACCG CG^G[AT]CCG
DrdI 3084 GACGTACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6658 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4536 GAATTC G^AATTC
FbaI 6507 TGATCA T^GATCA
FspI 3732 TGCGCA TGC^GCA
KpnI 624 GGTACC GGTAC^C
MluI 2053 ACGCGT A^CGCGT
PmaCI 4459 CACGTG CAC^GTG
PmlI 4459 CACGTG CAC^GTG
RsrII 2706 CGGACCG CG^G[AT]CCG
SalI 409 GTCGAC G^TCGAC
SapI 19 GAAGAGC GCTCTTC(1/4)
SbfI 5128 CCTGCAGG CCTGCA^GG
SgrAI 4397 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 5128 CCTGCAGG CCTGCA^GG
SspBI 4470 TGTACA T^GTACA
XbaI 2152 TCTAGA T^CTAGA
XmnI 275 GAATACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 409 GTCGAC GT^[AC][GT]AC
6205 GTCTAC
Aor51HI 2985 AGCGCT AGC^GCT
4338 AGCGCT
AvrII 4660 CCTAGG C^CTAGG
4881 CCTAGG
BamHI 4784 GGATCC G^GATCC
6395 GGATCC
BcgI 3793 CGAACAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6281 CGAACGCCCTGC
BglII 1215 AGATCT A^GATCT
1638 AGATCT
BlnI 4660 CCTAGG C^CTAGG
4881 CCTAGG
BlpI 4430 GCTGAGC GC^T[ACGT]AGC
5224 GCTCAGC
Bpu1102I 4430 GCTGAGC GC^T[ACGT]AGC
5224 GCTCAGC
BspHI 1422 TCATGA T^CATGA
2202 TCATGA
BspLU11I 3439 ACATGT A^CATGT
5768 ACATGT
BsrBI 1445 CCGCTC CCGCTC(-3/-3)
3846 GAGCGG
BsrDI 1555 CATTGC GCAATG(2/0)
3133 CATTGC
BssSI 2249 CACGAG CACGAG(-5/-1)
1337 CTCGTG
CelII 4430 GCTGAGC GC^T[ACGT]AGC
5224 GCTCAGC
Eco47III 2985 AGCGCT AGC^GCT
4338 AGCGCT
HincII 409 GTCGAC GT[CT]^[GA]AC
6085 GTCAAC
HindII 409 GTCGAC GT[CT]^[GA]AC
6085 GTCAAC
KspI 235 CCGCGG CCGC^GG
3707 CCGCGG
PvuI 3211 CGATCG CGAT^CG
5879 CGATCG
RcaI 1422 TCATGA T^CATGA
2202 TCATGA
SacII 235 CCGCGG CCGC^GG
3707 CCGCGG
ScaI 1951 AGTACT AGT^ACT
2478 AGTACT
SexAI 1368 ACCAGGT A^CC[AT]GGT
2457 ACCTGGT
SphI 303 GCATGC GCATG^C
1488 GCATGC
XcmI 1972 CCAGGGTCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4233 CCATTTCCGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PshAI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoI
XhoII XmaCI XmaI