##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
BbeI BciVI BglII BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FseI HpaI KasI KspI
MluI MroI NaeI NarI NgoMIV
NotI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2137 GACGTC GACGT^C
Acc65I 528 GGTACC G^GTACC
AccII 3373 CGCG CG^CG
Asp718I 528 GGTACC G^GTACC
BamHI 2424 GGATCC G^GATCC
BcgI 3709 GCAGGCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1794 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2814 GGTCTC GGTCTC(1/5)
BspHI 620 TCATGA T^CATGA
BspLU11I 3397 ACATGT A^CATGT
BssSI 21 CACGAG CACGAG(-5/-1)
Bst1107I 4144 GTATAC GTA^TAC
BstBI 209 TTCGAA TT^CGAA
BstEII 2697 GGTAACC G^GT[ACGT]ACC
BstPI 2697 GGTAACC G^GT[ACGT]ACC
BstUI 3373 CGCG CG^CG
BstZ17I 4144 GTATAC GTA^TAC
DraIII 4502 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2697 GGTAACC G^GT[ACGT]ACC
EcoRI 655 GAATTC G^AATTC
HaeII 369 AGCGCC [GA]GCGC^[CT]
HincII 1528 GTTGAC GT[CT]^[GA]AC
HindII 1528 GTTGAC GT[CT]^[GA]AC
KpnI 528 GGTACC GGTAC^C
MamI 1794 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2081 CAATTG C^AATTG
MunI 2081 CAATTG C^AATTG
MvnI 3373 CGCG CG^CG
NdeI 3284 CATATG CA^TATG
NruI 3372 TCGCGA TCG^CGA
NspV 209 TTCGAA TT^CGAA
RcaI 620 TCATGA T^CATGA
SacI 124 GAGCTC GAGCT^C
ScaI 2433 AGTACT AGT^ACT
SexAI 5176 ACCAGGT A^CC[AT]GGT
SfuI 209 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1491 GACGCC G[GA]^CG[CT]C
2137 GACGTC
AflII 5643 CTTAAG C^TTAAG
5914 CTTAAG
ApaI 1952 GGGCCC GGGCC^C
4907 GGGCCC
BalI 551 TGGCCA TGG^CCA
911 TGGCCA
BbrPI 3098 CACGTG CAC^GTG
4604 CACGTG
BbvCI 826 CCTCAGC CCTCAGC(-5/-2)
2206 GCTGAGG
BfrI 5643 CTTAAG C^TTAAG
5914 CTTAAG
BglI 274 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5833 GCCTTACAGGC
BsaAI 3098 CACGTG [CT]AC^GT[GA]
4604 CACGTG
BsaHI 1491 GACGCC G[GA]^CG[CT]C
2137 GACGTC
BsaWI 1814 TCCGGT [AT]^CCGG[AT]
3000 TCCGGT
BstAPI 1362 GCATAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1425 GCAGAGTGTGC
BstXI 1679 CCAGTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4911 CCACTGACTTGG
DraI 2803 TTTAAA TTT^AAA
2892 TTTAAA
DrdI 639 GACTGTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2843 GACGTTACAGTC
DsaI 914 CCATGG C^C[GA][CT]GG
1847 CCATGG
FauI 163 CCCGC CCCGC(4/6)
1140 GCGGG
HgaI 1491 GACGC GACGC(5/10)
852 GCGTC
Hin1I 1491 GACGCC G[GA]^CG[CT]C
2137 GACGTC
HindIII 1408 AAGCTT A^AGCTT
4582 AAGCTT
MluNI 551 TGGCCA TGG^CCA
911 TGGCCA
MscI 551 TGGCCA TGG^CCA
911 TGGCCA
NcoI 914 CCATGG C^CATGG
1847 CCATGG
NheI 2281 GCTAGC G^CTAGC
5142 GCTAGC
PmaCI 3098 CACGTG CAC^GTG
4604 CACGTG
PmlI 3098 CACGTG CAC^GTG
4604 CACGTG
SphI 980 GCATGC GCATG^C
2940 GCATGC
StuI 2516 AGGCCT AGG^CCT
2707 AGGCCT
XcmI 254 CCAGGTTTGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1964 CCAACTGGCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaI
AvaII AviII AvrII BanI BanII
BbsI BbvI BclI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
FspI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII XmaCI XmaI XmnI