##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccII AgeI Aor51HI AscI
BbrPI BclI BsaBI BsiEI BsiWI
BspDI BssHII BstBI BstUI ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRV FbaI HincII HindII HpaI
KspI MamI MluI MvnI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3504 GGTACC G^GTACC
AccI 303 GTATAC GT^[AC][GT]AC
AccIII 475 TCCGGA T^CCGGA
AclI 2987 AACGTT AA^CGTT
AflII 3594 CTTAAG C^TTAAG
AhdI 1978 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2738 ATTAAT AT^TAAT
AsnI 2738 ATTAAT AT^TAAT
Asp700I 1113 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3504 GGTACC G^GTACC
AspEI 1978 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4154 CCTAGG C^CTAGG
BamHI 4160 GGATCC G^GATCC
BciVI 3372 GGATAC GTATCC(6/5)
BfrI 3594 CTTAAG C^TTAAG
BlnI 4154 CCTAGG C^CTAGG
BsaAI 817 TACGTG [CT]AC^GT[GA]
BseAI 475 TCCGGA T^CCGGA
Bsp1407I 3360 TGTACA T^GTACA
BspEI 475 TCCGGA T^CCGGA
BspHI 5717 TCATGA T^CATGA
BspLU11I 4774 ACATGT A^CATGT
BsrGI 3360 TGTACA T^GTACA
Bst1107I 303 GTATAC GTA^TAC
BstZ17I 303 GTATAC GTA^TAC
DraIII 2186 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3878 GACATCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1978 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 451 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3579 GAATTC G^AATTC
FseI 2269 GGCCGGCC GGCCGG^CC
HindIII 3561 AAGCTT A^AGCTT
KpnI 3504 GGTACC GGTAC^C
MroI 475 TCCGGA T^CCGGA
NaeI 2270 GCCGGC GCC^GGC
NgoMIV 2270 GCCGGC G^CCGGC
PshAI 998 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2738 ATTAAT AT^TAAT
Psp1406I 2987 AACGTT AA^CGTT
RcaI 5717 TCATGA T^CATGA
SapI 1946 GCTCTTC GCTCTTC(1/4)
SexAI 62 ACCTGGT A^CC[AT]GGT
SphI 22 GCATGC GCATG^C
SspBI 3360 TGTACA T^GTACA
SspI 1256 AATATT AAT^ATT
StuI 1937 AGGCCT AGG^CCT
XmnI 1113 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 818 ACGTGT A^C[GA][CT]GT
4774 ACATGT
ApaI 4484 GGGCCC GGGCC^C
5997 GGGCCC
AvaI 455 CCCGAG C^[CT]CG[GA]G
1929 CTCGGG
BbeI 709 GGCGCC GGCGC^C
3528 GGCGCC
BbsI 972 GAAGAC GAAGAC(2/6)
5876 GAAGAC
BbvCI 1871 CCTCAGC CCTCAGC(-5/-2)
5351 GCTGAGG
BcgI 1224 CGAAGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2427 CGAGGACTTTGC
BpuAI 972 GAAGAC GAAGAC(2/6)
5876 GAAGAC
BsaWI 421 ACCGGA [AT]^CCGG[AT]
475 TCCGGA
BsmI 4353 GAATGC GAATGC(1/-1)
2892 GCATTC
BsoBI 455 CCCGAG C^[CT]CG[GA]G
1929 CTCGGG
BsrDI 5850 CATTGC GCAATG(2/0)
3761 GCAATG
BsrFI 688 ACCGGC [GA]^CCGG[CT]
2270 GCCGGC
BstEII 1287 GGTGACC G^GT[ACGT]ACC
3786 GGTCACC
BstPI 1287 GGTGACC G^GT[ACGT]ACC
3786 GGTCACC
Cfr10I 688 ACCGGC [GA]^CCGG[CT]
2270 GCCGGC
EarI 1947 CTCTTC CTCTTC(1/4)
4741 CTCTTC
EcoO65I 1287 GGTGACC G^GT[ACGT]ACC
3786 GGTCACC
EcoT22I 24 ATGCAT ATGCA^T
1392 ATGCAT
KasI 709 GGCGCC G^GCGCC
3528 GGCGCC
Ksp632I 1947 CTCTTC CTCTTC(1/4)
4741 CTCTTC
MfeI 1271 CAATTG C^AATTG
5063 CAATTG
MunI 1271 CAATTG C^AATTG
5063 CAATTG
NarI 709 GGCGCC GG^CGCC
3528 GGCGCC
NdeI 1706 CATATG CA^TATG
4908 CATATG
NsiI 24 ATGCAT ATGCA^T
1392 ATGCAT
PflMI 1491 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3434 CCACACTCTGG
SfoI 709 GGCGCC GGC^GCC
3528 GGCGCC
SmlI 522 CTCAAG C^T[CT][GA]AG
3594 CTTAAG
Van91I 1491 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3434 CCACACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AspHI AspI AvaII AviII
BalI BanI BanII BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XcmI XhoII