################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII AflII AscI BfrI BsiEI BsiWI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CpoI DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI HincII HindII HpaI MfeI MunI NaeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PmeI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 4496 GGTACC G^GTACC AccI 425 GTCTAC GT^[AC][GT]AC AccIII 3917 TCCGGA T^CCGGA AclI 4929 AACGTT AA^CGTT AgeI 965 ACCGGT A^CCGGT AhdI 1733 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 4032 CCTCAGG CC^T[ACGT]AGG Aor51HI 2717 AGCGCT AGC^GCT AseI 5420 ATTAAT AT^TAAT AsnI 5420 ATTAAT AT^TAAT Asp700I 4108 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4496 GGTACC G^GTACC AspEI 1733 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2640 TGCGCA TGC^GCA BbeI 6103 GGCGCC GGCGC^C BbrPI 815 CACGTG CAC^GTG BcgI 2643 GCAAACTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 6657 GGATAC GTATCC(6/5) BglI 2978 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 2447 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3917 TCCGGA T^CCGGA BsmBI 350 GAGACG CGTCTC(1/5) BspDI 791 ATCGAT AT^CGAT BspEI 3917 TCCGGA T^CCGGA Bsu36I 4032 CCTCAGG CC^T[ACGT]AGG ClaI 791 ATCGAT AT^CGAT Eam1105I 1733 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2717 AGCGCT AGC^GCT Eco81I 4032 CCTCAGG CC^T[ACGT]AGG FspI 2640 TGCGCA TGC^GCA KasI 6103 GGCGCC G^GCGCC KpnI 4496 GGTACC GGTAC^C KspI 313 CCGCGG CCGC^GG MamI 2447 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 2860 ACGCGT A^CGCGT MroI 3917 TCCGGA T^CCGGA NarI 6103 GGCGCC GG^CGCC NdeI 492 CATATG CA^TATG NruI 2401 TCGCGA TCG^CGA PinAI 965 ACCGGT A^CCGGT PmaCI 815 CACGTG CAC^GTG PmlI 815 CACGTG CAC^GTG PshBI 5420 ATTAAT AT^TAAT Psp1406I 4929 AACGTT AA^CGTT SacII 313 CCGCGG CCGC^GG SfoI 6103 GGCGCC GGC^GCC SmaI 2670 CCCGGG CCC^GGG SpeI 3808 ACTAGT A^CTAGT SwaI 6547 ATTTAAAT ATTT^AAAT XmaCI 2670 CCCGGG C^CCGGG XmaI 2670 CCCGGG C^CCGGG XmnI 4108 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 94 GGCGTC G[GA]^CG[CT]C 6103 GGCGCC ApaI 197 GGGCCC GGGCC^C 2253 GGGCCC AspI 1180 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2605 GACTGTGTC AvrII 5104 CCTAGG C^CTAGG 6632 CCTAGG BamHI 247 GGATCC G^GATCC 3284 GGATCC BlnI 5104 CCTAGG C^CTAGG 6632 CCTAGG BlpI 2482 GCTCAGC GC^T[ACGT]AGC 5831 GCTGAGC Bpu1102I 2482 GCTCAGC GC^T[ACGT]AGC 5831 GCTGAGC BsaHI 94 GGCGTC G[GA]^CG[CT]C 6103 GGCGCC BsaWI 965 ACCGGT [AT]^CCGG[AT] 3917 TCCGGA Bsp1407I 2004 TGTACA T^GTACA 6471 TGTACA BspHI 709 TCATGA T^CATGA 4387 TCATGA BspLU11I 1066 ACATGT A^CATGT 5572 ACATGT BsrDI 4089 CATTGC GCAATG(2/0) 5582 CATTGC BsrFI 279 GCCGGT [GA]^CCGG[CT] 965 ACCGGT BsrGI 2004 TGTACA T^GTACA 6471 TGTACA BssSI 1612 CACGAG CACGAG(-5/-1) 2039 CTCGTG CelII 2482 GCTCAGC GC^T[ACGT]AGC 5831 GCTGAGC Cfr10I 279 GCCGGT [GA]^CCGG[CT] 965 ACCGGT DraIII 5340 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 5902 CACATGGTG EcoNI 2227 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4937 CCTTTAAGAGG Hin1I 94 GGCGTC G[GA]^CG[CT]C 6103 GGCGCC HindIII 4890 AAGCTT A^AGCTT 6072 AAGCTT PflFI 1180 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2605 GACTGTGTC PshAI 3046 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 6138 GACACTTGTC RcaI 709 TCATGA T^CATGA 4387 TCATGA SacI 1598 GAGCTC GAGCT^C 4472 GAGCTC SapI 1402 GAAGAGC GCTCTTC(1/4) 3081 GCTCTTC SspBI 2004 TGTACA T^GTACA 6471 TGTACA StuI 1302 AGGCCT AGG^CCT 2868 AGGCCT Tth111I 1180 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2605 GACTGTGTC XbaI 2612 TCTAGA T^CTAGA 4970 TCTAGA XcmI 690 CCAAGACCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6107 CCACGGGGACTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII