##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AflII AscI BfrI BsiEI
BsiWI BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CpoI
DrdI EagI EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI HincII HindII
HpaI MfeI MunI NaeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PmeI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 4496 GGTACC G^GTACC
AccI 425 GTCTAC GT^[AC][GT]AC
AccIII 3917 TCCGGA T^CCGGA
AclI 4929 AACGTT AA^CGTT
AgeI 965 ACCGGT A^CCGGT
AhdI 1733 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4032 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2717 AGCGCT AGC^GCT
AseI 5420 ATTAAT AT^TAAT
AsnI 5420 ATTAAT AT^TAAT
Asp700I 4108 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4496 GGTACC G^GTACC
AspEI 1733 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2640 TGCGCA TGC^GCA
BbeI 6103 GGCGCC GGCGC^C
BbrPI 815 CACGTG CAC^GTG
BcgI 2643 GCAAACTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 6657 GGATAC GTATCC(6/5)
BglI 2978 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2447 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3917 TCCGGA T^CCGGA
BsmBI 350 GAGACG CGTCTC(1/5)
BspDI 791 ATCGAT AT^CGAT
BspEI 3917 TCCGGA T^CCGGA
Bsu36I 4032 CCTCAGG CC^T[ACGT]AGG
ClaI 791 ATCGAT AT^CGAT
Eam1105I 1733 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2717 AGCGCT AGC^GCT
Eco81I 4032 CCTCAGG CC^T[ACGT]AGG
FspI 2640 TGCGCA TGC^GCA
KasI 6103 GGCGCC G^GCGCC
KpnI 4496 GGTACC GGTAC^C
KspI 313 CCGCGG CCGC^GG
MamI 2447 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2860 ACGCGT A^CGCGT
MroI 3917 TCCGGA T^CCGGA
NarI 6103 GGCGCC GG^CGCC
NdeI 492 CATATG CA^TATG
NruI 2401 TCGCGA TCG^CGA
PinAI 965 ACCGGT A^CCGGT
PmaCI 815 CACGTG CAC^GTG
PmlI 815 CACGTG CAC^GTG
PshBI 5420 ATTAAT AT^TAAT
Psp1406I 4929 AACGTT AA^CGTT
SacII 313 CCGCGG CCGC^GG
SfoI 6103 GGCGCC GGC^GCC
SmaI 2670 CCCGGG CCC^GGG
SpeI 3808 ACTAGT A^CTAGT
SwaI 6547 ATTTAAAT ATTT^AAAT
XmaCI 2670 CCCGGG C^CCGGG
XmaI 2670 CCCGGG C^CCGGG
XmnI 4108 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 94 GGCGTC G[GA]^CG[CT]C
6103 GGCGCC
ApaI 197 GGGCCC GGGCC^C
2253 GGGCCC
AspI 1180 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2605 GACTGTGTC
AvrII 5104 CCTAGG C^CTAGG
6632 CCTAGG
BamHI 247 GGATCC G^GATCC
3284 GGATCC
BlnI 5104 CCTAGG C^CTAGG
6632 CCTAGG
BlpI 2482 GCTCAGC GC^T[ACGT]AGC
5831 GCTGAGC
Bpu1102I 2482 GCTCAGC GC^T[ACGT]AGC
5831 GCTGAGC
BsaHI 94 GGCGTC G[GA]^CG[CT]C
6103 GGCGCC
BsaWI 965 ACCGGT [AT]^CCGG[AT]
3917 TCCGGA
Bsp1407I 2004 TGTACA T^GTACA
6471 TGTACA
BspHI 709 TCATGA T^CATGA
4387 TCATGA
BspLU11I 1066 ACATGT A^CATGT
5572 ACATGT
BsrDI 4089 CATTGC GCAATG(2/0)
5582 CATTGC
BsrFI 279 GCCGGT [GA]^CCGG[CT]
965 ACCGGT
BsrGI 2004 TGTACA T^GTACA
6471 TGTACA
BssSI 1612 CACGAG CACGAG(-5/-1)
2039 CTCGTG
CelII 2482 GCTCAGC GC^T[ACGT]AGC
5831 GCTGAGC
Cfr10I 279 GCCGGT [GA]^CCGG[CT]
965 ACCGGT
DraIII 5340 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
5902 CACATGGTG
EcoNI 2227 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4937 CCTTTAAGAGG
Hin1I 94 GGCGTC G[GA]^CG[CT]C
6103 GGCGCC
HindIII 4890 AAGCTT A^AGCTT
6072 AAGCTT
PflFI 1180 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2605 GACTGTGTC
PshAI 3046 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6138 GACACTTGTC
RcaI 709 TCATGA T^CATGA
4387 TCATGA
SacI 1598 GAGCTC GAGCT^C
4472 GAGCTC
SapI 1402 GAAGAGC GCTCTTC(1/4)
3081 GCTCTTC
SspBI 2004 TGTACA T^GTACA
6471 TGTACA
StuI 1302 AGGCCT AGG^CCT
2868 AGGCCT
Tth111I 1180 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2605 GACTGTGTC
XbaI 2612 TCTAGA T^CTAGA
4970 TCTAGA
XcmI 690 CCAAGACCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6107 CCACGGGGACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII