################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## Acc65I AccII AclI AflII AgeI AscI AseI AsnI Asp718I AvrII BfrI BlnI BsiWI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CpoI DraI EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FseI KpnI KspI MluI MvnI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 2479 GACGTC GACGT^C AccIII 201 TCCGGA T^CCGGA AcsI 5666 AAATTC [GA]^AATT[CT] Alw44I 2351 GTGCAC G^TGCAC Aor51HI 2336 AGCGCT AGC^GCT ApaLI 2351 GTGCAC G^TGCAC ApoI 5666 AAATTC [GA]^AATT[CT] Asp700I 5001 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2600 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3498 TGCGCA TGC^GCA BbeI 2514 GGCGCC GGCGC^C BbvCI 4826 CCTCAGC CCTCAGC(-5/-2) BclI 2535 TGATCA T^GATCA BglII 2943 AGATCT A^GATCT BsaBI 3439 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 201 TCCGGA [AT]^CCGG[AT] BseAI 201 TCCGGA T^CCGGA BsiEI 1479 CGACCG CG[GA][CT]^CG BsmI 5524 GCATTC GAATGC(1/-1) Bsp1407I 4234 TGTACA T^GTACA BspDI 3002 ATCGAT AT^CGAT BspEI 201 TCCGGA T^CCGGA BspHI 5366 TCATGA T^CATGA BsrGI 4234 TGTACA T^GTACA ClaI 3002 ATCGAT AT^CGAT DrdI 4364 GACTCACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2336 AGCGCT AGC^GCT EcoRV 3293 GATATC GAT^ATC FbaI 2535 TGATCA T^GATCA FspI 3498 TGCGCA TGC^GCA HgaI 1210 GACGC GACGC(5/10) HindIII 412 AAGCTT A^AGCTT HpaI 101 GTTAAC GTT^AAC KasI 2514 GGCGCC G^GCGCC MamI 3439 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4690 CAATTG C^AATTG MroI 201 TCCGGA T^CCGGA MunI 4690 CAATTG C^AATTG NaeI 4135 GCCGGC GCC^GGC NarI 2514 GGCGCC GG^CGCC NdeI 935 CATATG CA^TATG NgoMIV 4135 GCCGGC G^CCGGC PflFI 2600 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 5366 TCATGA T^CATGA SbfI 2320 CCTGCAGG CCTGCA^GG SfoI 2514 GGCGCC GGC^GCC SphI 2922 GCATGC GCATG^C Sse8387I 2320 CCTGCAGG CCTGCA^GG SspBI 4234 TGTACA T^GTACA Tth111I 2600 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 5001 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AccI 520 GTCTAC GT^[AC][GT]AC 1715 GTAGAC BciVI 922 GTATCC GTATCC(6/5) 4949 GTATCC BsrDI 3535 CATTGC GCAATG(2/0) 4119 GCAATG BstAPI 3501 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4875 GCACCCTCTGC PshAI 4688 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5307 GACACGTGTC SexAI 987 ACCTGGT A^CC[AT]GGT 3348 ACCAGGT SfiI 4559 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5103 GGCCCATGTGGCC SmlI 2225 CTCAAG C^T[CT][GA]AG 4001 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AciI AcyI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApaI AspEI AspHI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI