##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AscI AseI AsnI Asp718I AvrII
BfrI BlnI BsiWI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CpoI DraI EagI EclXI
Eco52I EcoO65I EcoRI EcoT22I FseI
KpnI KspI MluI MvnI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2479 GACGTC GACGT^C
AccIII 201 TCCGGA T^CCGGA
AcsI 5666 AAATTC [GA]^AATT[CT]
Alw44I 2351 GTGCAC G^TGCAC
Aor51HI 2336 AGCGCT AGC^GCT
ApaLI 2351 GTGCAC G^TGCAC
ApoI 5666 AAATTC [GA]^AATT[CT]
Asp700I 5001 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2600 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3498 TGCGCA TGC^GCA
BbeI 2514 GGCGCC GGCGC^C
BbvCI 4826 CCTCAGC CCTCAGC(-5/-2)
BclI 2535 TGATCA T^GATCA
BglII 2943 AGATCT A^GATCT
BsaBI 3439 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 201 TCCGGA [AT]^CCGG[AT]
BseAI 201 TCCGGA T^CCGGA
BsiEI 1479 CGACCG CG[GA][CT]^CG
BsmI 5524 GCATTC GAATGC(1/-1)
Bsp1407I 4234 TGTACA T^GTACA
BspDI 3002 ATCGAT AT^CGAT
BspEI 201 TCCGGA T^CCGGA
BspHI 5366 TCATGA T^CATGA
BsrGI 4234 TGTACA T^GTACA
ClaI 3002 ATCGAT AT^CGAT
DrdI 4364 GACTCACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2336 AGCGCT AGC^GCT
EcoRV 3293 GATATC GAT^ATC
FbaI 2535 TGATCA T^GATCA
FspI 3498 TGCGCA TGC^GCA
HgaI 1210 GACGC GACGC(5/10)
HindIII 412 AAGCTT A^AGCTT
HpaI 101 GTTAAC GTT^AAC
KasI 2514 GGCGCC G^GCGCC
MamI 3439 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4690 CAATTG C^AATTG
MroI 201 TCCGGA T^CCGGA
MunI 4690 CAATTG C^AATTG
NaeI 4135 GCCGGC GCC^GGC
NarI 2514 GGCGCC GG^CGCC
NdeI 935 CATATG CA^TATG
NgoMIV 4135 GCCGGC G^CCGGC
PflFI 2600 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 5366 TCATGA T^CATGA
SbfI 2320 CCTGCAGG CCTGCA^GG
SfoI 2514 GGCGCC GGC^GCC
SphI 2922 GCATGC GCATG^C
Sse8387I 2320 CCTGCAGG CCTGCA^GG
SspBI 4234 TGTACA T^GTACA
Tth111I 2600 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 5001 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AccI 520 GTCTAC GT^[AC][GT]AC
1715 GTAGAC
BciVI 922 GTATCC GTATCC(6/5)
4949 GTATCC
BsrDI 3535 CATTGC GCAATG(2/0)
4119 GCAATG
BstAPI 3501 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4875 GCACCCTCTGC
PshAI 4688 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5307 GACACGTGTC
SexAI 987 ACCTGGT A^CC[AT]GGT
3348 ACCAGGT
SfiI 4559 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5103 GGCCCATGTGGCC
SmlI 2225 CTCAAG C^T[CT][GA]AG
4001 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AciI AcyI AfaI AflIII AhdI
AluI AlwI AlwNI AocI ApaI
AspEI AspHI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI