##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
ApaI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BbeI BbsI
BclI BfrI BlnI BpuAI BsaBI
BseAI BsiWI BsmBI BspDI BspEI
BssHII Bst1107I BstBI BstXI BstZ17I
ClaI CpoI Eam1105I EcoRV FbaI
FseI KasI KpnI MamI MluI
MroI NarI NruI NspV PacI
PinAI PmeI PshAI PshBI PvuI
RsrII SalI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 2814 AACGTT AA^CGTT
Alw44I 1868 GTGCAC G^TGCAC
AlwI 495 GATCC GGATC(4/5)
Aor51HI 262 AGCGCT AGC^GCT
ApaLI 1868 GTGCAC G^TGCAC
Asp700I 1729 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4249 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 603 TGCGCA TGC^GCA
BbrPI 2384 CACGTG CAC^GTG
BbvCI 2001 CCTCAGC CCTCAGC(-5/-2)
BciVI 4783 GTATCC GTATCC(6/5)
BglII 1983 AGATCT A^GATCT
Bsp1407I 2584 TGTACA T^GTACA
BspMI 2071 ACCTGC ACCTGC(4/8)
BsrGI 2584 TGTACA T^GTACA
BssSI 919 CACGAG CACGAG(-5/-1)
BstEII 292 GGTCACC G^GT[ACGT]ACC
BstPI 292 GGTCACC G^GT[ACGT]ACC
BstYI 1983 AGATCT [GA]^GATC[CT]
DraIII 1400 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2364 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 262 AGCGCT AGC^GCT
EcoO65I 292 GGTCACC G^GT[ACGT]ACC
EcoRI 2679 GAATTC G^AATTC
FspI 603 TGCGCA TGC^GCA
HpaI 2219 GTTAAC GTT^AAC
KspI 232 CCGCGG CCGC^GG
MflI 1983 AGATCT [GA]^GATC[CT]
NotI 464 GCGGCCGC GC^GGCCGC
PaeR7I 573 CTCGAG C^TCGAG
PflFI 4249 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2384 CACGTG CAC^GTG
PmlI 2384 CACGTG CAC^GTG
Psp1406I 2814 AACGTT AA^CGTT
PvuII 3763 CAGCTG CAG^CTG
SacII 232 CCGCGG CCGC^GG
SapI 2177 GCTCTTC GCTCTTC(1/4)
SexAI 2828 ACCTGGT A^CC[AT]GGT
SfiI 202 GGCCCCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1776 CCCGGG CCC^GGG
SphI 2247 GCATGC GCATG^C
SspBI 2584 TGTACA T^GTACA
SwaI 4606 ATTTAAAT ATTT^AAAT
Tth111I 4249 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 573 CTCGAG C^TCGAG
XhoII 1983 AGATCT [GA]^GATC[CT]
XmaCI 1776 CCCGGG C^CCGGG
XmaI 1776 CCCGGG C^CCGGG
XmnI 1729 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 2414 CCTCAGG CC^T[ACGT]AGG
2807 CCTAAGG
BanI 236 GGTGCC G^G[CT][GA]CC
2334 GGTGCC
BsaAI 2384 CACGTG [CT]AC^GT[GA]
3356 TACGTG
BsgI 3899 CTGCAC GTGCAG(16/14)
4444 CTGCAC
BspHI 2675 TCATGA T^CATGA
3755 TCATGA
Bsu36I 2414 CCTCAGG CC^T[ACGT]AGG
2807 CCTAAGG
DraII 2327 GGGTCCT [GA]G^G[ACGT]CC[CT]
2998 AGGACCT
Eco81I 2414 CCTCAGG CC^T[ACGT]AGG
2807 CCTAAGG
EcoNI 1162 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1607 CCTGAAACAGG
EcoO109I 2327 GGGTCCT [GA]G^G[ACGT]CC[CT]
2998 AGGACCT
HindIII 2492 AAGCTT A^AGCTT
4592 AAGCTT
MfeI 3946 CAATTG C^AATTG
4708 CAATTG
MunI 3946 CAATTG C^AATTG
4708 CAATTG
NcoI 2437 CCATGG C^CATGG
2742 CCATGG
NdeI 2449 CATATG CA^TATG
2559 CATATG
NheI 472 GCTAGC G^CTAGC
727 GCTAGC
PpuMI 2327 GGGTCCT [GA]G^G[AT]CC[CT]
2998 AGGACCT
RcaI 2675 TCATGA T^CATGA
3755 TCATGA
SspI 3890 AATATT AAT^ATT
4856 AATATT
XbaI 3436 TCTAGA T^CTAGA
3734 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwNI
ApoI AspHI AvaI AvaII BalI
BanII BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BsaWI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DsaI EaeI EagI
EarI EclXI Eco52I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NsiI
NspI PflMI PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI