##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI AseI AsnI Asp718I BbeI
BbvCI BglII BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrFI
BsrGI BssHII BstBI Cfr10I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I FseI HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI SspI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 507 GACGTC GACGT^C
AflII 4673 CTTAAG C^TTAAG
AspI 3137 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1599 TGCGCA TGC^GCA
BamHI 2634 GGATCC G^GATCC
BbrPI 5040 CACGTG CAC^GTG
BcgI 2203 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4673 CTTAAG C^TTAAG
BsaBI 2380 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 2703 TCATGA T^CATGA
BspLU11I 2961 ACATGT A^CATGT
BssSI 5674 CTCGTG CACGAG(-5/-1)
Bst1107I 2909 GTATAC GTA^TAC
BstEII 4395 GGTCACC G^GT[ACGT]ACC
BstPI 4395 GGTCACC G^GT[ACGT]ACC
BstZ17I 2909 GTATAC GTA^TAC
EcoO65I 4395 GGTCACC G^GT[ACGT]ACC
EcoRI 4467 GAATTC G^AATTC
EcoRV 459 GATATC GAT^ATC
EcoT22I 5427 ATGCAT ATGCA^T
FspI 1599 TGCGCA TGC^GCA
HaeII 297 AGCGCC [GA]GCGC^[CT]
HincII 489 GTCAAC GT[CT]^[GA]AC
HindII 489 GTCAAC GT[CT]^[GA]AC
MamI 2380 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5847 CAATTG C^AATTG
MunI 5847 CAATTG C^AATTG
NsiI 5427 ATGCAT ATGCA^T
PaeR7I 2372 CTCGAG C^TCGAG
PflFI 3137 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5040 CACGTG CAC^GTG
PmlI 5040 CACGTG CAC^GTG
RcaI 2703 TCATGA T^CATGA
ScaI 5793 AGTACT AGT^ACT
SfiI 2191 GGCCTCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2694 ACTAGT A^CTAGT
SwaI 1772 ATTTAAAT ATTT^AAAT
Tth111I 3137 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5474 TCTAGA T^CTAGA
XhoI 2372 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2909 GTATAC GT^[AC][GT]AC
5755 GTCTAC
AccII 333 CGCG CG^CG
430 CGCG
AcyI 366 GGCGTC G[GA]^CG[CT]C
507 GACGTC
Alw44I 1592 GTGCAC G^TGCAC
4759 GTGCAC
ApaLI 1592 GTGCAC G^TGCAC
4759 GTGCAC
Asp700I 921 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3908 GAAATGTTTC
BalI 69 TGGCCA TGG^CCA
4417 TGGCCA
BanI 2832 GGCACC G^G[CT][GA]CC
4508 GGTGCC
BciVI 4219 GTATCC GTATCC(6/5)
5587 GTATCC
BclI 3188 TGATCA T^GATCA
5256 TGATCA
BlpI 4141 GCTCAGC GC^T[ACGT]AGC
5282 GCTCAGC
Bpu1102I 4141 GCTCAGC GC^T[ACGT]AGC
5282 GCTCAGC
BsaHI 366 GGCGTC G[GA]^CG[CT]C
507 GACGTC
BsmBI 2445 CGTCTC CGTCTC(1/5)
4717 CGTCTC
BsrBI 345 GAGCGG CCGCTC(-3/-3)
4136 GAGCGG
BstAPI 547 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1523 GCATTACCTGC
BstUI 333 CGCG CG^CG
430 CGCG
BstXI 1006 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1210 CCAGATACGTGG
BstYI 2634 GGATCC [GA]^GATC[CT]
3066 GGATCT
CelII 4141 GCTCAGC GC^T[ACGT]AGC
5282 GCTCAGC
FbaI 3188 TGATCA T^GATCA
5256 TGATCA
Hin1I 366 GGCGTC G[GA]^CG[CT]C
507 GACGTC
MflI 2634 GGATCC [GA]^GATC[CT]
3066 GGATCT
MluNI 69 TGGCCA TGG^CCA
4417 TGGCCA
MscI 69 TGGCCA TGG^CCA
4417 TGGCCA
MvnI 333 CGCG CG^CG
430 CGCG
NdeI 2282 CATATG CA^TATG
4767 CATATG
SapI 100 GAAGAGC GCTCTTC(1/4)
4272 GCTCTTC
SmaI 475 CCCGGG CCC^GGG
2226 CCCGGG
XcmI 427 CCACGCGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3989 CCAATAGAGGCTTGG
XhoII 2634 GGATCC [GA]^GATC[CT]
3066 GGATCT
XmaCI 475 CCCGGG C^CCGGG
2226 CCCGGG
XmaI 475 CCCGGG C^CCGGG
2226 CCCGGG
XmnI 921 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3908 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AhdI
AluI AlwI AlwNI AocI ApaI
ApoI AspEI AspHI AvaI AvaII
AvrII BanII BbsI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I