##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I ApaLI AscI AspI
AviII AvrII BbeI BcgI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII Cfr10I CpoI DraI
EagI EclXI Eco52I EcoNI EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 2654 GGTACC G^GTACC
AccI 1831 GTCTAC GT^[AC][GT]AC
AflII 796 CTTAAG C^TTAAG
AhdI 484 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1708 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1562 AGCGCT AGC^GCT
ApaI 825 GGGCCC GGGCC^C
AseI 2948 ATTAAT AT^TAAT
AsnI 2948 ATTAAT AT^TAAT
Asp700I 574 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2654 GGTACC G^GTACC
AspEI 484 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1301 GGATCC G^GATCC
BbrPI 2861 CACGTG CAC^GTG
BciVI 1984 GGATAC GTATCC(6/5)
BclI 1284 TGATCA T^GATCA
BfrI 796 CTTAAG C^TTAAG
BglI 172 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 767 AGATCT A^GATCT
BsaBI 858 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2240 GCATTC GAATGC(1/-1)
Bsp1407I 645 TGTACA T^GTACA
BspDI 721 ATCGAT AT^CGAT
BspLU11I 1133 ACATGT A^CATGT
BsrDI 1341 CATTGC GCAATG(2/0)
BsrGI 645 TGTACA T^GTACA
BstEII 1713 GGTAACC G^GT[ACGT]ACC
BstPI 1713 GGTAACC G^GT[ACGT]ACC
Bsu36I 1708 CCTGAGG CC^T[ACGT]AGG
ClaI 721 ATCGAT AT^CGAT
Eam1105I 484 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1562 AGCGCT AGC^GCT
Eco81I 1708 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1713 GGTAACC G^GT[ACGT]ACC
FbaI 1284 TGATCA T^GATCA
HaeII 1562 AGCGCT [GA]GCGC^[CT]
HgaI 1293 GACGC GACGC(5/10)
KpnI 2654 GGTACC GGTAC^C
MamI 858 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 447 CCATGG C^CATGG
NdeI 1253 CATATG CA^TATG
PacI 2945 TTAATTAA TTAAT^TAA
PflMI 863 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2861 CACGTG CAC^GTG
PmlI 2861 CACGTG CAC^GTG
PshBI 2948 ATTAAT AT^TAAT
SapI 2340 GCTCTTC GCTCTTC(1/4)
SexAI 1031 ACCTGGT A^CC[AT]GGT
SmaI 2784 CCCGGG CCC^GGG
SmlI 796 CTTAAG C^T[CT][GA]AG
SphI 1418 GCATGC GCATG^C
SspBI 645 TGTACA T^GTACA
StuI 2702 AGGCCT AGG^CCT
Van91I 863 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2784 CCCGGG C^CCGGG
XmaI 2784 CCCGGG C^CCGGG
XmnI 574 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 223 GAATTT [GA]^AATT[CT]
1243 AAATTC
AflIII 1133 ACATGT A^C[GA][CT]GT
2862 ACGTGT
ApoI 223 GAATTT [GA]^AATT[CT]
1243 AAATTC
AspHI 1099 GTGCTC G[AT]GC[AT]^C
1600 GTGCTC
AvaI 476 CTCGGG C^[CT]CG[GA]G
2784 CCCGGG
BalI 1635 TGGCCA TGG^CCA
1643 TGGCCA
BbsI 1377 GAAGAC GAAGAC(2/6)
2066 GAAGAC
BpuAI 1377 GAAGAC GAAGAC(2/6)
2066 GAAGAC
BsaAI 1825 TACGTG [CT]AC^GT[GA]
2861 CACGTG
BsaI 482 GAGACC GGTCTC(1/5)
540 GAGACC
BseRI 358 GAGGAG GAGGAG(10/8)
1000 GAGGAG
BsiHKAI 1099 GTGCTC G[AT]GC[AT]^C
1600 GTGCTC
BsmBI 35 CGTCTC CGTCTC(1/5)
1291 GAGACG
BsoBI 476 CTCGGG C^[CT]CG[GA]G
2784 CCCGGG
BspHI 663 TCATGA T^CATGA
1793 TCATGA
BstAPI 741 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2471 GCACTGGCTGC
DraIII 901 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1030 CACCTGGTG
MluNI 1635 TGGCCA TGG^CCA
1643 TGGCCA
MscI 1635 TGGCCA TGG^CCA
1643 TGGCCA
PstI 569 CTGCAG CTGCA^G
2364 CTGCAG
RcaI 663 TCATGA T^CATGA
1793 TCATGA
ScaI 1409 AGTACT AGT^ACT
1627 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII