##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AgeI AscI AseI
AsnI AviII BbeI BcgI BciVI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI EclXI Eco52I FseI FspI
KasI KspI MamI MluI MroI
NarI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 757 GACGTC GACGT^C
AflII 140 CTTAAG C^TTAAG
Alw44I 4672 GTGCAC G^TGCAC
ApaLI 4672 GTGCAC G^TGCAC
AvaI 4548 CTCGGG C^[CT]CG[GA]G
AvrII 299 CCTAGG C^CTAGG
BamHI 23 GGATCC G^GATCC
BfrI 140 CTTAAG C^TTAAG
BlnI 299 CCTAGG C^CTAGG
BsmBI 4532 GAGACG CGTCTC(1/5)
BsoBI 4548 CTCGGG C^[CT]CG[GA]G
BspLU11I 3606 ACATGT A^CATGT
BssSI 731 CTCGTG CACGAG(-5/-1)
DsaI 5900 CCATGG C^C[GA][CT]GG
EcoRV 5802 GATATC GAT^ATC
EcoT22I 3546 ATGCAT ATGCA^T
MfeI 955 CAATTG C^AATTG
MunI 955 CAATTG C^AATTG
NaeI 5295 GCCGGC GCC^GGC
NcoI 5900 CCATGG C^CATGG
NgoMIV 5295 GCCGGC G^CCGGC
NsiI 3546 ATGCAT ATGCA^T
PshAI 2717 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 728 GAGCTC GAGCT^C
ScaI 3414 AGTACT AGT^ACT
XbaI 6238 TCTAGA T^CTAGA
XcmI 2404 CCAGCCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 747 GGTACC G^GTACC
5533 GGTACC
AocI 1354 CCTCAGG CC^T[ACGT]AGG
4653 CCTTAGG
Asp700I 1253 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2995 GAATTTGTTC
Asp718I 747 GGTACC G^GTACC
5533 GGTACC
AspI 2053 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4714 GACAGTGTC
BbrPI 3760 CACGTG CAC^GTG
3815 CACGTG
BclI 544 TGATCA T^GATCA
1167 TGATCA
BglII 1080 AGATCT A^GATCT
2494 AGATCT
Bsp1407I 3348 TGTACA T^GTACA
3764 TGTACA
BsrFI 2094 ACCGGC [GA]^CCGG[CT]
5295 GCCGGC
BsrGI 3348 TGTACA T^GTACA
3764 TGTACA
BstEII 1291 GGTGACC G^GT[ACGT]ACC
6127 GGTGACC
BstPI 1291 GGTGACC G^GT[ACGT]ACC
6127 GGTGACC
Bsu36I 1354 CCTCAGG CC^T[ACGT]AGG
4653 CCTTAGG
Cfr10I 2094 ACCGGC [GA]^CCGG[CT]
5295 GCCGGC
DraI 4449 TTTAAA TTT^AAA
4487 TTTAAA
Eco81I 1354 CCTCAGG CC^T[ACGT]AGG
4653 CCTTAGG
EcoNI 2759 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5649 CCTCCAATAGG
EcoO65I 1291 GGTGACC G^GT[ACGT]ACC
6127 GGTGACC
FbaI 544 TGATCA T^GATCA
1167 TGATCA
HindIII 3191 AAGCTT A^AGCTT
4508 AAGCTT
HpaI 2365 GTTAAC GTT^AAC
3836 GTTAAC
KpnI 747 GGTACC GGTAC^C
5533 GGTACC
NheI 2596 GCTAGC G^CTAGC
5905 GCTAGC
PflFI 2053 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4714 GACAGTGTC
PmaCI 3760 CACGTG CAC^GTG
3815 CACGTG
PmlI 3760 CACGTG CAC^GTG
3815 CACGTG
SmlI 140 CTTAAG C^T[CT][GA]AG
2561 CTTGAG
SpeI 2289 ACTAGT A^CTAGT
3728 ACTAGT
SspBI 3348 TGTACA T^GTACA
3764 TGTACA
Tth111I 2053 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4714 GACAGTGTC
XmnI 1253 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2995 GAATTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI Aor51HI ApaI ApoI AspEI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DrdI EaeI Eam1105I
EarI Eco47III EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII