################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccIII AclI AgeI AscI AseI AsnI AviII BbeI BcgI BciVI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I FseI FspI KasI KspI MamI MluI MroI NarI NdeI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AatII 757 GACGTC GACGT^C AflII 140 CTTAAG C^TTAAG Alw44I 4672 GTGCAC G^TGCAC ApaLI 4672 GTGCAC G^TGCAC AvaI 4548 CTCGGG C^[CT]CG[GA]G AvrII 299 CCTAGG C^CTAGG BamHI 23 GGATCC G^GATCC BfrI 140 CTTAAG C^TTAAG BlnI 299 CCTAGG C^CTAGG BsmBI 4532 GAGACG CGTCTC(1/5) BsoBI 4548 CTCGGG C^[CT]CG[GA]G BspLU11I 3606 ACATGT A^CATGT BssSI 731 CTCGTG CACGAG(-5/-1) DsaI 5900 CCATGG C^C[GA][CT]GG EcoRV 5802 GATATC GAT^ATC EcoT22I 3546 ATGCAT ATGCA^T MfeI 955 CAATTG C^AATTG MunI 955 CAATTG C^AATTG NaeI 5295 GCCGGC GCC^GGC NcoI 5900 CCATGG C^CATGG NgoMIV 5295 GCCGGC G^CCGGC NsiI 3546 ATGCAT ATGCA^T PshAI 2717 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 728 GAGCTC GAGCT^C ScaI 3414 AGTACT AGT^ACT XbaI 6238 TCTAGA T^CTAGA XcmI 2404 CCAGCCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 747 GGTACC G^GTACC 5533 GGTACC AocI 1354 CCTCAGG CC^T[ACGT]AGG 4653 CCTTAGG Asp700I 1253 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2995 GAATTTGTTC Asp718I 747 GGTACC G^GTACC 5533 GGTACC AspI 2053 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4714 GACAGTGTC BbrPI 3760 CACGTG CAC^GTG 3815 CACGTG BclI 544 TGATCA T^GATCA 1167 TGATCA BglII 1080 AGATCT A^GATCT 2494 AGATCT Bsp1407I 3348 TGTACA T^GTACA 3764 TGTACA BsrFI 2094 ACCGGC [GA]^CCGG[CT] 5295 GCCGGC BsrGI 3348 TGTACA T^GTACA 3764 TGTACA BstEII 1291 GGTGACC G^GT[ACGT]ACC 6127 GGTGACC BstPI 1291 GGTGACC G^GT[ACGT]ACC 6127 GGTGACC Bsu36I 1354 CCTCAGG CC^T[ACGT]AGG 4653 CCTTAGG Cfr10I 2094 ACCGGC [GA]^CCGG[CT] 5295 GCCGGC DraI 4449 TTTAAA TTT^AAA 4487 TTTAAA Eco81I 1354 CCTCAGG CC^T[ACGT]AGG 4653 CCTTAGG EcoNI 2759 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5649 CCTCCAATAGG EcoO65I 1291 GGTGACC G^GT[ACGT]ACC 6127 GGTGACC FbaI 544 TGATCA T^GATCA 1167 TGATCA HindIII 3191 AAGCTT A^AGCTT 4508 AAGCTT HpaI 2365 GTTAAC GTT^AAC 3836 GTTAAC KpnI 747 GGTACC GGTAC^C 5533 GGTACC NheI 2596 GCTAGC G^CTAGC 5905 GCTAGC PflFI 2053 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4714 GACAGTGTC PmaCI 3760 CACGTG CAC^GTG 3815 CACGTG PmlI 3760 CACGTG CAC^GTG 3815 CACGTG SmlI 140 CTTAAG C^T[CT][GA]AG 2561 CTTGAG SpeI 2289 ACTAGT A^CTAGT 3728 ACTAGT SspBI 3348 TGTACA T^GTACA 3764 TGTACA Tth111I 2053 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4714 GACAGTGTC XmnI 1253 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2995 GAATTTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI Aor51HI ApaI ApoI AspEI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DrdI EaeI Eam1105I EarI Eco47III EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII