##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BclI BfrI
BlnI BsaBI BseAI BsiWI Bsp1407I
BspEI BsrGI BssHII Bst1107I BstZ17I
CpoI DraI DrdI Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
ScaI SexAI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2457 GACGTC GACGT^C
AflIII 5632 ACATGT A^C[GA][CT]GT
Aor51HI 4164 AGCGCT AGC^GCT
AviII 1965 TGCGCA TGC^GCA
BbrPI 3935 CACGTG CAC^GTG
BsaAI 3935 CACGTG [CT]AC^GT[GA]
BsaWI 1213 ACCGGA [AT]^CCGG[AT]
BspDI 2633 ATCGAT AT^CGAT
BspLU11I 5632 ACATGT A^CATGT
BstBI 5853 TTCGAA TT^CGAA
BstEII 2143 GGTGACC G^GT[ACGT]ACC
BstPI 2143 GGTGACC G^GT[ACGT]ACC
ClaI 2633 ATCGAT AT^CGAT
EagI 4935 CGGCCG C^GGCCG
EclXI 4935 CGGCCG C^GGCCG
Eco47III 4164 AGCGCT AGC^GCT
Eco52I 4935 CGGCCG C^GGCCG
EcoO65I 2143 GGTGACC G^GT[ACGT]ACC
FspI 1965 TGCGCA TGC^GCA
KspI 2437 CCGCGG CCGC^GG
NspV 5853 TTCGAA TT^CGAA
PmaCI 3935 CACGTG CAC^GTG
PmlI 3935 CACGTG CAC^GTG
SacII 2437 CCGCGG CCGC^GG
SalI 5978 GTCGAC G^TCGAC
SfiI 4060 GGCCACTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5853 TTCGAA TT^CGAA
SgrAI 2837 CACCGGCG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 4004 GTAGAC GT^[AC][GT]AC
5978 GTCGAC
AcsI 3956 AAATTC [GA]^AATT[CT]
4478 AAATTC
Alw44I 1284 GTGCAC G^TGCAC
1651 GTGCAC
ApaLI 1284 GTGCAC G^TGCAC
1651 GTGCAC
ApoI 3956 AAATTC [GA]^AATT[CT]
4478 AAATTC
AspI 170 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5128 GACTCAGTC
BbeI 3198 GGCGCC GGCGC^C
4734 GGCGCC
BcgI 1573 GCAGCAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3526 GCAATTCTATCG
BciVI 3879 GGATAC GTATCC(6/5)
4465 GGATAC
BsiEI 43 CGACCG CG[GA][CT]^CG
4935 CGGCCG
BsmBI 677 CGTCTC CGTCTC(1/5)
5268 GAGACG
BsmI 5722 GAATGC GAATGC(1/-1)
2714 GCATTC
BspHI 2403 TCATGA T^CATGA
3330 TCATGA
BssSI 1287 CACGAG CACGAG(-5/-1)
5382 CACGAG
DraIII 2692 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5160 CACCCAGTG
HgaI 2417 GACGC GACGC(5/10)
2568 GACGC
KasI 3198 GGCGCC G^GCGCC
4734 GGCGCC
NarI 3198 GGCGCC GG^CGCC
4734 GGCGCC
PflFI 170 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5128 GACTCAGTC
RcaI 2403 TCATGA T^CATGA
3330 TCATGA
SbfI 2956 CCTGCAGG CCTGCA^GG
3820 CCTGCAGG
SfoI 3198 GGCGCC GGC^GCC
4734 GGCGCC
Sse8387I 2956 CCTGCAGG CCTGCA^GG
3820 CCTGCAGG
Tth111I 170 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5128 GACTCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI