################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BamHI BclI BfrI BlnI BsaBI BseAI BsiWI Bsp1407I BspEI BsrGI BssHII Bst1107I BstZ17I CpoI DraI DrdI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII ScaI SexAI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AatII 2457 GACGTC GACGT^C AflIII 5632 ACATGT A^C[GA][CT]GT Aor51HI 4164 AGCGCT AGC^GCT AviII 1965 TGCGCA TGC^GCA BbrPI 3935 CACGTG CAC^GTG BsaAI 3935 CACGTG [CT]AC^GT[GA] BsaWI 1213 ACCGGA [AT]^CCGG[AT] BspDI 2633 ATCGAT AT^CGAT BspLU11I 5632 ACATGT A^CATGT BstBI 5853 TTCGAA TT^CGAA BstEII 2143 GGTGACC G^GT[ACGT]ACC BstPI 2143 GGTGACC G^GT[ACGT]ACC ClaI 2633 ATCGAT AT^CGAT EagI 4935 CGGCCG C^GGCCG EclXI 4935 CGGCCG C^GGCCG Eco47III 4164 AGCGCT AGC^GCT Eco52I 4935 CGGCCG C^GGCCG EcoO65I 2143 GGTGACC G^GT[ACGT]ACC FspI 1965 TGCGCA TGC^GCA KspI 2437 CCGCGG CCGC^GG NspV 5853 TTCGAA TT^CGAA PmaCI 3935 CACGTG CAC^GTG PmlI 3935 CACGTG CAC^GTG SacII 2437 CCGCGG CCGC^GG SalI 5978 GTCGAC G^TCGAC SfiI 4060 GGCCACTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 5853 TTCGAA TT^CGAA SgrAI 2837 CACCGGCG C[GA]^CCGG[CT]G ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 4004 GTAGAC GT^[AC][GT]AC 5978 GTCGAC AcsI 3956 AAATTC [GA]^AATT[CT] 4478 AAATTC Alw44I 1284 GTGCAC G^TGCAC 1651 GTGCAC ApaLI 1284 GTGCAC G^TGCAC 1651 GTGCAC ApoI 3956 AAATTC [GA]^AATT[CT] 4478 AAATTC AspI 170 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5128 GACTCAGTC BbeI 3198 GGCGCC GGCGC^C 4734 GGCGCC BcgI 1573 GCAGCAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3526 GCAATTCTATCG BciVI 3879 GGATAC GTATCC(6/5) 4465 GGATAC BsiEI 43 CGACCG CG[GA][CT]^CG 4935 CGGCCG BsmBI 677 CGTCTC CGTCTC(1/5) 5268 GAGACG BsmI 5722 GAATGC GAATGC(1/-1) 2714 GCATTC BspHI 2403 TCATGA T^CATGA 3330 TCATGA BssSI 1287 CACGAG CACGAG(-5/-1) 5382 CACGAG DraIII 2692 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 5160 CACCCAGTG HgaI 2417 GACGC GACGC(5/10) 2568 GACGC KasI 3198 GGCGCC G^GCGCC 4734 GGCGCC NarI 3198 GGCGCC GG^CGCC 4734 GGCGCC PflFI 170 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5128 GACTCAGTC RcaI 2403 TCATGA T^CATGA 3330 TCATGA SbfI 2956 CCTGCAGG CCTGCA^GG 3820 CCTGCAGG SfoI 3198 GGCGCC GGC^GCC 4734 GGCGCC Sse8387I 2956 CCTGCAGG CCTGCA^GG 3820 CCTGCAGG Tth111I 170 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5128 GACTCAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI