##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AflII AgeI AscI AseI AsnI
Asp700I AviII BbrPI BfrI BsaBI
BsiWI BspHI BssHII BstBI BstEII
BstPI CpoI EagI EclXI Eco52I
EcoO65I EcoRV FseI FspI HpaI
MamI MfeI MluI MunI NdeI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 62 GACGTC GACGT^C
AccIII 3785 TCCGGA T^CCGGA
Aor51HI 4593 AGCGCT AGC^GCT
ApaI 3448 GGGCCC GGGCC^C
AvrII 4228 CCTAGG C^CTAGG
BciVI 3493 GGATAC GTATCC(6/5)
BclI 3697 TGATCA T^GATCA
BglI 3857 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4943 AGATCT A^GATCT
BlnI 4228 CCTAGG C^CTAGG
BsaAI 1704 CACGTA [CT]AC^GT[GA]
BseAI 3785 TCCGGA T^CCGGA
BsmBI 60 GAGACG CGTCTC(1/5)
BspDI 5643 ATCGAT AT^CGAT
BspEI 3785 TCCGGA T^CCGGA
BspLU11I 1218 ACATGT A^CATGT
Bst1107I 754 GTATAC GTA^TAC
BstZ17I 754 GTATAC GTA^TAC
ClaI 5643 ATCGAT AT^CGAT
DraI 3505 TTTAAA TTT^AAA
Eco47III 4593 AGCGCT AGC^GCT
EcoT22I 4143 ATGCAT ATGCA^T
FbaI 3697 TGATCA T^GATCA
HindIII 2752 AAGCTT A^AGCTT
KspI 14 CCGCGG CCGC^GG
MroI 3785 TCCGGA T^CCGGA
NsiI 4143 ATGCAT ATGCA^T
SacI 577 GAGCTC GAGCT^C
SacII 14 CCGCGG CCGC^GG
SmaI 3873 CCCGGG CCC^GGG
SphI 4501 GCATGC GCATG^C
SspI 5892 AATATT AAT^ATT
XmaCI 3873 CCCGGG C^CCGGG
XmaI 3873 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 285 GGTACC G^GTACC
4218 GGTACC
AccI 754 GTATAC GT^[AC][GT]AC
773 GTAGAC
AclI 765 AACGTT AA^CGTT
4104 AACGTT
Alw44I 3223 GTGCAC G^TGCAC
5814 GTGCAC
ApaLI 3223 GTGCAC G^TGCAC
5814 GTGCAC
Asp718I 285 GGTACC G^GTACC
4218 GGTACC
AspI 2093 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3704 GACTCTGTC
BbvCI 3232 CCTCAGC CCTCAGC(-5/-2)
5520 GCTGAGG
BcgI 5575 CGACCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
898 GCAAGACCTTCG
BsiEI 2975 CGGTCG CG[GA][CT]^CG
4240 CGACCG
BstXI 1245 CCACCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2178 CCAGTGAAGTGG
DrdI 428 GACACCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
977 GACATTGAGGTC
EcoRI 104 GAATTC G^AATTC
1589 GAATTC
HincII 458 GTCAAC GT[CT]^[GA]AC
1628 GTTGAC
HindII 458 GTCAAC GT[CT]^[GA]AC
1628 GTTGAC
KpnI 285 GGTACC GGTAC^C
4218 GGTACC
NcoI 177 CCATGG C^CATGG
1650 CCATGG
NheI 2723 GCTAGC G^CTAGC
5221 GCTAGC
PflFI 2093 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3704 GACTCTGTC
PshAI 4190 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4639 GACCAAGGTC
Psp1406I 765 AACGTT AA^CGTT
4104 AACGTT
StuI 918 AGGCCT AGG^CCT
1464 AGGCCT
Tth111I 2093 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3704 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApoI AspEI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII