##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AocI Aor51HI ApaI AscI
Asp718I AvaI AviII BanI BbeI
BbrPI BbvCI BcgI BciVI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BstAPI BstBI
BstUI Bsu36I CelII CfoI Cfr10I
ClaI CpoI Eco47III Eco81I FseI
FspI HaeII HhaI Hin1I HinP1I
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 2641 AACGTT AA^CGTT
AhdI 4945 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1352 GTGCAC G^TGCAC
ApaLI 1352 GTGCAC G^TGCAC
AspEI 4945 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2233 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2362 CCTAGG C^CTAGG
BcnI 4916 CCCGG CC^[GC]GG
BlnI 2362 CCTAGG C^CTAGG
BsaAI 5844 TACGTG [CT]AC^GT[GA]
BsiEI 6031 CGGCCG CG[GA][CT]^CG
BspMI 2191 GCAGGT ACCTGC(4/8)
BsrDI 1276 CATTGC GCAATG(2/0)
BssSI 5291 CTCGTG CACGAG(-5/-1)
Bst1107I 1096 GTATAC GTA^TAC
BstEII 3628 GGTGACC G^GT[ACGT]ACC
BstPI 3628 GGTGACC G^GT[ACGT]ACC
BstZ17I 1096 GTATAC GTA^TAC
DraIII 1572 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2214 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 6031 CGGCCG C^GGCCG
Eam1105I 4945 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 6031 CGGCCG C^GGCCG
Eco52I 6031 CGGCCG C^GGCCG
EcoO65I 3628 GGTGACC G^GT[ACGT]ACC
EcoRV 1484 GATATC GAT^ATC
FauI 5395 CCCGC CCCGC(4/6)
HgaI 3445 GACGC GACGC(5/10)
HpaI 6363 GTTAAC GTT^AAC
MfeI 6857 CAATTG C^AATTG
MunI 6857 CAATTG C^AATTG
NciI 4916 CCCGG CC^[GC]GG
NheI 2389 GCTAGC G^CTAGC
PflFI 2233 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2641 AACGTT AA^CGTT
SalI 749 GTCGAC G^TCGAC
SexAI 2234 ACCTGGT A^CC[AT]GGT
StuI 41 AGGCCT AGG^CCT
SwaI 3639 ATTTAAAT ATTT^AAAT
Tth111I 2233 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 864 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
4011 CAGAGACTG
BamHI 17 GGATCC G^GATCC
3503 GGATCC
BbsI 5120 GTCTTC GAAGAC(2/6)
5253 GTCTTC
BclI 340 TGATCA T^GATCA
4314 TGATCA
BpuAI 5120 GTCTTC GAAGAC(2/6)
5253 GTCTTC
BsgI 945 CTGCAC GTGCAG(16/14)
1569 CTGCAC
BstXI 3266 CCATGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5033 CCAATCATATGG
EcoRI 2528 GAATTC G^AATTC
6735 GAATTC
EcoT22I 111 ATGCAT ATGCA^T
5883 ATGCAT
FbaI 340 TGATCA T^GATCA
4314 TGATCA
HapII 83 CCGG C^CGG
4917 CCGG
HincII 749 GTCGAC GT[CT]^[GA]AC
6363 GTTAAC
HindII 749 GTCGAC GT[CT]^[GA]AC
6363 GTTAAC
HpaII 83 CCGG C^CGG
4917 CCGG
MspA1I 660 CAGCTG C[AC]G^C[GT]G
3967 CAGCTG
MspI 83 CCGG C^CGG
4917 CCGG
NsiI 111 ATGCAT ATGCA^T
5883 ATGCAT
PpuMI 6194 AGGTCCT [GA]G^G[AT]CC[CT]
6867 GGGTCCT
PstI 482 CTGCAG CTGCA^G
2451 CTGCAG
PvuII 660 CAGCTG CAG^CTG
3967 CAGCTG
ScaI 516 AGTACT AGT^ACT
2173 AGTACT
SpeI 400 ACTAGT A^CTAGT
874 ACTAGT
SphI 3958 GCATGC GCATG^C
5881 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AvaII BalI
BanII BbvI BfaI BfrI BglI
BglII BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmnI