##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccI AccIII AclI AgeI AscI
AseI AsnI AvrII BamHI BbeI
BcgI BclI BlnI BseAI BsiWI
BspDI BspEI BsrBI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
EcoO65I EcoRV EcoT22I FbaI HindIII
KasI MluI MroI NarI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfoI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 4812 GAATTC [GA]^AATT[CT]
AhdI 1642 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2085 GTGCAC G^TGCAC
Aor51HI 1118 AGCGCT AGC^GCT
ApaI 4617 GGGCCC GGGCC^C
ApaLI 2085 GTGCAC G^TGCAC
ApoI 4812 GAATTC [GA]^AATT[CT]
AspEI 1642 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 521 TGCGCA TGC^GCA
BsaWI 2093 TCCGGT [AT]^CCGG[AT]
BsmI 2323 GAATGC GAATGC(1/-1)
BspHI 3604 TCATGA T^CATGA
BspLU11I 576 ACATGT A^CATGT
BssHII 4971 GCGCGC G^CGCGC
BssSI 1131 CACGAG CACGAG(-5/-1)
BstBI 712 TTCGAA TT^CGAA
DraIII 1522 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4889 GACTGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1642 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1118 AGCGCT AGC^GCT
EcoRI 4812 GAATTC G^AATTC
FseI 1032 GGCCGGCC GGCCGG^CC
FspI 521 TGCGCA TGC^GCA
HpaI 2498 GTTAAC GTT^AAC
KspI 143 CCGCGG CCGC^GG
MfeI 1174 CAATTG C^AATTG
MunI 1174 CAATTG C^AATTG
NcoI 4316 CCATGG C^CATGG
NheI 2577 GCTAGC G^CTAGC
NspV 712 TTCGAA TT^CGAA
RcaI 3604 TCATGA T^CATGA
SacI 1981 GAGCTC GAGCT^C
SacII 143 CCGCGG CCGC^GG
SexAI 1012 ACCAGGT A^CC[AT]GGT
SfiI 1036 GGCCGCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 712 TTCGAA TT^CGAA
SmaI 887 CCCGGG CCC^GGG
SphI 3422 GCATGC GCATG^C
XmaCI 887 CCCGGG C^CCGGG
XmaI 887 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 471 GACGTC GACGT^C
2735 GACGTC
Acc65I 1535 GGTACC G^GTACC
4688 GGTACC
AflII 2550 CTTAAG C^TTAAG
3637 CTTAAG
AflIII 576 ACATGT A^C[GA][CT]GT
2502 ACACGT
Asp718I 1535 GGTACC G^GTACC
4688 GGTACC
BbsI 810 GAAGAC GAAGAC(2/6)
2364 GTCTTC
BbvCI 1425 CCTCAGC CCTCAGC(-5/-2)
163 GCTGAGG
BciVI 1349 GGATAC GTATCC(6/5)
2718 GTATCC
BfrI 2550 CTTAAG C^TTAAG
3637 CTTAAG
BpuAI 810 GAAGAC GAAGAC(2/6)
2364 GTCTTC
BsaBI 249 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5070 GATACTGATC
BsiEI 1031 CGGCCG CG[GA][CT]^CG
1035 CGGCCG
BsmBI 258 CGTCTC CGTCTC(1/5)
852 CGTCTC
EagI 1031 CGGCCG C^GGCCG
1035 CGGCCG
EclXI 1031 CGGCCG C^GGCCG
1035 CGGCCG
Eco52I 1031 CGGCCG C^GGCCG
1035 CGGCCG
EcoNI 1007 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1751 CCTCTCAGAGG
KpnI 1535 GGTACC GGTAC^C
4688 GGTACC
MamI 249 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5070 GATACTGATC
NdeI 3085 CATATG CA^TATG
4072 CATATG
PflMI 1521 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1575 CCAGGATCTGG
PshAI 2160 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4342 GACAGCAGTC
SapI 1731 GAAGAGC GCTCTTC(1/4)
1443 GCTCTTC
Van91I 1521 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1575 CCAGGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XbaI
XhoII XmnI