##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AspEI AspI
AviII AvrII BamHI BciVI BclI
BfrI BglII BlnI BseAI BsiEI
BsiWI BsmI Bsp1407I BspEI BspLU11I
BsrBI BsrGI BstBI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FbaI FseI FspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NotI NruI NspV
PflFI PflMI PinAI PmeI PshAI
Psp1406I PvuI RsrII SalI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2776 GGTACC G^GTACC
AcsI 138 AAATTC [GA]^AATT[CT]
Alw44I 2548 GTGCAC G^TGCAC
ApaLI 2548 GTGCAC G^TGCAC
ApoI 138 AAATTC [GA]^AATT[CT]
AseI 3947 ATTAAT AT^TAAT
AsnI 3947 ATTAAT AT^TAAT
Asp718I 2776 GGTACC G^GTACC
BbeI 3005 GGCGCC GGCGC^C
BcgI 789 GCAGTGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 2180 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 333 ACCGGA [AT]^CCGG[AT]
BsmBI 560 GAGACG CGTCTC(1/5)
BspDI 408 ATCGAT AT^CGAT
BssHII 13 GCGCGC G^CGCGC
Bst1107I 1972 GTATAC GTA^TAC
BstEII 1510 GGTGACC G^GT[ACGT]ACC
BstPI 1510 GGTGACC G^GT[ACGT]ACC
BstYI 238 GGATCT [GA]^GATC[CT]
BstZ17I 1972 GTATAC GTA^TAC
ClaI 408 ATCGAT AT^CGAT
EcoNI 1613 CCTTCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1510 GGTGACC G^GT[ACGT]ACC
EcoRV 411 GATATC GAT^ATC
HincII 1424 GTTAAC GT[CT]^[GA]AC
HindII 1424 GTTAAC GT[CT]^[GA]AC
HpaI 1424 GTTAAC GTT^AAC
KasI 3005 GGCGCC G^GCGCC
KpnI 2776 GGTACC GGTAC^C
MamI 2180 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 238 GGATCT [GA]^GATC[CT]
NarI 3005 GGCGCC GG^CGCC
NcoI 1368 CCATGG C^CATGG
NheI 3334 GCTAGC G^CTAGC
PacI 674 TTAATTAA TTAAT^TAA
PaeR7I 1617 CTCGAG C^TCGAG
PshBI 3947 ATTAAT AT^TAAT
SacI 2237 GAGCTC GAGCT^C
SexAI 2343 ACCAGGT A^CC[AT]GGT
SfoI 3005 GGCGCC GGC^GCC
XbaI 4011 TCTAGA T^CTAGA
XhoI 1617 CTCGAG C^TCGAG
XhoII 238 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 812 GACGCC G[GA]^CG[CT]C
3005 GGCGCC
AflIII 596 ACGTGT A^C[GA][CT]GT
4006 ACGTGT
Asp700I 2321 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3382 GAAACAGTTC
BbrPI 595 CACGTG CAC^GTG
647 CACGTG
BlpI 1828 GCTCAGC GC^T[ACGT]AGC
2297 GCTCAGC
Bpu1102I 1828 GCTCAGC GC^T[ACGT]AGC
2297 GCTCAGC
BsaHI 812 GACGCC G[GA]^CG[CT]C
3005 GGCGCC
BspHI 415 TCATGA T^CATGA
2557 TCATGA
BsrDI 982 CATTGC GCAATG(2/0)
2316 GCAATG
BsrFI 1870 GCCGGT [GA]^CCGG[CT]
2002 GCCGGT
BssSI 2709 CACGAG CACGAG(-5/-1)
3857 CACGAG
BstAPI 2059 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2506 GCACCATTTGC
CelII 1828 GCTCAGC GC^T[ACGT]AGC
2297 GCTCAGC
Cfr10I 1870 GCCGGT [GA]^CCGG[CT]
2002 GCCGGT
DrdI 3062 GACCTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3295 GACGGAGGGGTC
EcoT22I 920 ATGCAT ATGCA^T
2519 ATGCAT
Hin1I 812 GACGCC G[GA]^CG[CT]C
3005 GGCGCC
KspI 113 CCGCGG CCGC^GG
2877 CCGCGG
NsiI 920 ATGCAT ATGCA^T
2519 ATGCAT
NspI 2219 ACATGC [GA]CATG^[CT]
2420 GCATGT
PmaCI 595 CACGTG CAC^GTG
647 CACGTG
PmlI 595 CACGTG CAC^GTG
647 CACGTG
PpuMI 44 GGGTCCC [GA]G^G[AT]CC[CT]
1414 GGGTCCC
PvuII 217 CAGCTG CAG^CTG
2028 CAGCTG
RcaI 415 TCATGA T^CATGA
2557 TCATGA
SacII 113 CCGCGG CCGC^GG
2877 CCGCGG
SapI 2239 GCTCTTC GCTCTTC(1/4)
2674 GCTCTTC
SbfI 1499 CCTGCAGG CCTGCA^GG
1661 CCTGCAGG
ScaI 267 AGTACT AGT^ACT
771 AGTACT
Sse8387I 1499 CCTGCAGG CCTGCA^GG
1661 CCTGCAGG
XmnI 2321 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3382 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI