################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Aor51HI AscI AspEI AspI AviII AvrII BamHI BciVI BclI BfrI BglII BlnI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BspLU11I BsrBI BsrGI BstBI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI FbaI FseI FspI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NspV PflFI PflMI PinAI PmeI PshAI Psp1406I PvuI RsrII SalI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 2776 GGTACC G^GTACC AcsI 138 AAATTC [GA]^AATT[CT] Alw44I 2548 GTGCAC G^TGCAC ApaLI 2548 GTGCAC G^TGCAC ApoI 138 AAATTC [GA]^AATT[CT] AseI 3947 ATTAAT AT^TAAT AsnI 3947 ATTAAT AT^TAAT Asp718I 2776 GGTACC G^GTACC BbeI 3005 GGCGCC GGCGC^C BcgI 789 GCAGTGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 2180 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 333 ACCGGA [AT]^CCGG[AT] BsmBI 560 GAGACG CGTCTC(1/5) BspDI 408 ATCGAT AT^CGAT BssHII 13 GCGCGC G^CGCGC Bst1107I 1972 GTATAC GTA^TAC BstEII 1510 GGTGACC G^GT[ACGT]ACC BstPI 1510 GGTGACC G^GT[ACGT]ACC BstYI 238 GGATCT [GA]^GATC[CT] BstZ17I 1972 GTATAC GTA^TAC ClaI 408 ATCGAT AT^CGAT EcoNI 1613 CCTTCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1510 GGTGACC G^GT[ACGT]ACC EcoRV 411 GATATC GAT^ATC HincII 1424 GTTAAC GT[CT]^[GA]AC HindII 1424 GTTAAC GT[CT]^[GA]AC HpaI 1424 GTTAAC GTT^AAC KasI 3005 GGCGCC G^GCGCC KpnI 2776 GGTACC GGTAC^C MamI 2180 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 238 GGATCT [GA]^GATC[CT] NarI 3005 GGCGCC GG^CGCC NcoI 1368 CCATGG C^CATGG NheI 3334 GCTAGC G^CTAGC PacI 674 TTAATTAA TTAAT^TAA PaeR7I 1617 CTCGAG C^TCGAG PshBI 3947 ATTAAT AT^TAAT SacI 2237 GAGCTC GAGCT^C SexAI 2343 ACCAGGT A^CC[AT]GGT SfoI 3005 GGCGCC GGC^GCC XbaI 4011 TCTAGA T^CTAGA XhoI 1617 CTCGAG C^TCGAG XhoII 238 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 812 GACGCC G[GA]^CG[CT]C 3005 GGCGCC AflIII 596 ACGTGT A^C[GA][CT]GT 4006 ACGTGT Asp700I 2321 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3382 GAAACAGTTC BbrPI 595 CACGTG CAC^GTG 647 CACGTG BlpI 1828 GCTCAGC GC^T[ACGT]AGC 2297 GCTCAGC Bpu1102I 1828 GCTCAGC GC^T[ACGT]AGC 2297 GCTCAGC BsaHI 812 GACGCC G[GA]^CG[CT]C 3005 GGCGCC BspHI 415 TCATGA T^CATGA 2557 TCATGA BsrDI 982 CATTGC GCAATG(2/0) 2316 GCAATG BsrFI 1870 GCCGGT [GA]^CCGG[CT] 2002 GCCGGT BssSI 2709 CACGAG CACGAG(-5/-1) 3857 CACGAG BstAPI 2059 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2506 GCACCATTTGC CelII 1828 GCTCAGC GC^T[ACGT]AGC 2297 GCTCAGC Cfr10I 1870 GCCGGT [GA]^CCGG[CT] 2002 GCCGGT DrdI 3062 GACCTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3295 GACGGAGGGGTC EcoT22I 920 ATGCAT ATGCA^T 2519 ATGCAT Hin1I 812 GACGCC G[GA]^CG[CT]C 3005 GGCGCC KspI 113 CCGCGG CCGC^GG 2877 CCGCGG NsiI 920 ATGCAT ATGCA^T 2519 ATGCAT NspI 2219 ACATGC [GA]CATG^[CT] 2420 GCATGT PmaCI 595 CACGTG CAC^GTG 647 CACGTG PmlI 595 CACGTG CAC^GTG 647 CACGTG PpuMI 44 GGGTCCC [GA]G^G[AT]CC[CT] 1414 GGGTCCC PvuII 217 CAGCTG CAG^CTG 2028 CAGCTG RcaI 415 TCATGA T^CATGA 2557 TCATGA SacII 113 CCGCGG CCGC^GG 2877 CCGCGG SapI 2239 GCTCTTC GCTCTTC(1/4) 2674 GCTCTTC SbfI 1499 CCTGCAGG CCTGCA^GG 1661 CCTGCAGG ScaI 267 AGTACT AGT^ACT 771 AGTACT Sse8387I 1499 CCTGCAGG CCTGCA^GG 1661 CCTGCAGG XmnI 2321 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3382 GAAACAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI