##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
BamHI BclI BglI BsaBI BsiWI
Bsp1407I BsrGI Bst1107I BstBI BstZ17I
CpoI DraI EcoRI EcoT22I FbaI
FseI HindIII HpaI KspI MamI
MfeI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 4986 GACGTC GACGT^C
AflII 2265 CTTAAG C^TTAAG
Alw44I 2159 GTGCAC G^TGCAC
Aor51HI 4072 AGCGCT AGC^GCT
ApaLI 2159 GTGCAC G^TGCAC
Asp700I 158 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1511 CCTAGG C^CTAGG
BbrPI 1230 CACGTG CAC^GTG
BbvCI 2804 CCTCAGC CCTCAGC(-5/-2)
BcgI 1751 CGAGTGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2265 CTTAAG C^TTAAG
BlnI 1511 CCTAGG C^CTAGG
BsaAI 1230 CACGTG [CT]AC^GT[GA]
BspDI 1290 ATCGAT AT^CGAT
BssHII 2525 GCGCGC G^CGCGC
ClaI 1290 ATCGAT AT^CGAT
DraIII 1101 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4072 AGCGCT AGC^GCT
EcoRV 1607 GATATC GAT^ATC
MluI 1187 ACGCGT A^CGCGT
NheI 660 GCTAGC G^CTAGC
PmaCI 1230 CACGTG CAC^GTG
PmlI 1230 CACGTG CAC^GTG
PshAI 936 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4432 CCTGCAGG CCTGCA^GG
Sse8387I 4432 CCTGCAGG CCTGCA^GG
XmnI 158 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3021 GGTACC G^GTACC
3453 GGTACC
AccIII 135 TCCGGA T^CCGGA
2338 TCCGGA
AocI 1547 CCTGAGG CC^T[ACGT]AGG
2559 CCTCAGG
Asp718I 3021 GGTACC G^GTACC
3453 GGTACC
BbeI 2074 GGCGCC GGCGC^C
3976 GGCGCC
BglII 202 AGATCT A^GATCT
372 AGATCT
BlpI 3613 GCTCAGC GC^T[ACGT]AGC
5038 GCTCAGC
Bpu1102I 3613 GCTCAGC GC^T[ACGT]AGC
5038 GCTCAGC
BsaWI 135 TCCGGA [AT]^CCGG[AT]
2338 TCCGGA
BseAI 135 TCCGGA T^CCGGA
2338 TCCGGA
BsiEI 3111 CGGCCG CG[GA][CT]^CG
3583 CGGCCG
BspEI 135 TCCGGA T^CCGGA
2338 TCCGGA
BspLU11I 1981 ACATGT A^CATGT
3103 ACATGT
BsrBI 2141 CCGCTC CCGCTC(-3/-3)
3305 CCGCTC
BsrDI 1268 CATTGC GCAATG(2/0)
2405 CATTGC
BssSI 4575 CACGAG CACGAG(-5/-1)
4586 CTCGTG
BstAPI 1100 GCACAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1901 GCAGCCTGTGC
BstEII 3185 GGTCACC G^GT[ACGT]ACC
4266 GGTGACC
BstPI 3185 GGTCACC G^GT[ACGT]ACC
4266 GGTGACC
Bsu36I 1547 CCTGAGG CC^T[ACGT]AGG
2559 CCTCAGG
CelII 3613 GCTCAGC GC^T[ACGT]AGC
5038 GCTCAGC
DrdI 1227 GACCACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4986 GACGTCACAGTC
EagI 3111 CGGCCG C^GGCCG
3583 CGGCCG
EclXI 3111 CGGCCG C^GGCCG
3583 CGGCCG
Eco52I 3111 CGGCCG C^GGCCG
3583 CGGCCG
Eco81I 1547 CCTGAGG CC^T[ACGT]AGG
2559 CCTCAGG
EcoO65I 3185 GGTCACC G^GT[ACGT]ACC
4266 GGTGACC
HincII 1467 GTTGAC GT[CT]^[GA]AC
2874 GTCAAC
HindII 1467 GTTGAC GT[CT]^[GA]AC
2874 GTCAAC
KasI 2074 GGCGCC G^GCGCC
3976 GGCGCC
KpnI 3021 GGTACC GGTAC^C
3453 GGTACC
MroI 135 TCCGGA T^CCGGA
2338 TCCGGA
NarI 2074 GGCGCC GG^CGCC
3976 GGCGCC
SacI 563 GAGCTC GAGCT^C
1573 GAGCTC
SapI 65 GCTCTTC GCTCTTC(1/4)
311 GCTCTTC
ScaI 2269 AGTACT AGT^ACT
4966 AGTACT
SfoI 2074 GGCGCC GGC^GCC
3976 GGCGCC
TfiI 2335 GATTC G^A[AT]TC
4594 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI ApaI ApoI AspEI AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI