##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AspEI AvrII BamHI BcgI
BlnI BseAI BsiEI BsiWI BspDI
BspEI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
HpaI MfeI MluI MroI MunI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PshAI Psp1406I PvuI
RsrII SbfI ScaI SfiI SfuI
SnaBI SpeI Sse8387I StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 839 GACGTC GACGT^C
Acc65I 1186 GGTACC G^GTACC
AflII 2246 CTTAAG C^TTAAG
Alw44I 734 GTGCAC G^TGCAC
ApaLI 734 GTGCAC G^TGCAC
AseI 3506 ATTAAT AT^TAAT
AsnI 3506 ATTAAT AT^TAAT
Asp700I 1406 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1186 GGTACC G^GTACC
AspI 2035 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5023 TGCGCA TGC^GCA
BbrPI 96 CACGTG CAC^GTG
BclI 854 TGATCA T^GATCA
BfrI 2246 CTTAAG C^TTAAG
BglI 4771 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 841 CGTCTC CGTCTC(1/5)
Bsp1407I 5041 TGTACA T^GTACA
BspHI 1903 TCATGA T^CATGA
BspLU11I 798 ACATGT A^CATGT
BsrBI 5226 CCGCTC CCGCTC(-3/-3)
BsrGI 5041 TGTACA T^GTACA
BstXI 1176 CCAGTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2452 GACTACGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 854 TGATCA T^GATCA
FspI 5023 TGCGCA TGC^GCA
HincII 836 GTCGAC GT[CT]^[GA]AC
HindII 836 GTCGAC GT[CT]^[GA]AC
HindIII 5246 AAGCTT A^AGCTT
KpnI 1186 GGTACC GGTAC^C
KspI 64 CCGCGG CCGC^GG
NheI 165 GCTAGC G^CTAGC
PflFI 2035 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4043 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 96 CACGTG CAC^GTG
PmeI 768 GTTTAAAC GTTT^AAAC
PmlI 96 CACGTG CAC^GTG
PpuMI 2116 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3506 ATTAAT AT^TAAT
RcaI 1903 TCATGA T^CATGA
SacII 64 CCGCGG CCGC^GG
SalI 836 GTCGAC G^TCGAC
SexAI 3287 ACCTGGT A^CC[AT]GGT
SgrAI 87 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2796 CCCGGG CCC^GGG
SphI 4689 GCATGC GCATG^C
SspBI 5041 TGTACA T^GTACA
SspI 4021 AATATT AAT^ATT
Tth111I 2035 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4043 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2796 CCCGGG C^CCGGG
XmaI 2796 CCCGGG C^CCGGG
XmnI 1406 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 764 ACACGT A^C[GA][CT]GT
798 ACATGT
AocI 3053 CCTGAGG CC^T[ACGT]AGG
5066 CCTTAGG
ApaI 59 GGGCCC GGGCC^C
363 GGGCCC
BbeI 38 GGCGCC GGCGC^C
323 GGCGCC
BciVI 1532 GGATAC GTATCC(6/5)
4731 GGATAC
BglII 1723 AGATCT A^GATCT
1921 AGATCT
BlpI 3155 GCTCAGC GC^T[ACGT]AGC
3610 GCTCAGC
Bpu1102I 3155 GCTCAGC GC^T[ACGT]AGC
3610 GCTCAGC
BsaAI 96 CACGTG [CT]AC^GT[GA]
1329 TACGTG
BsaWI 2253 ACCGGA [AT]^CCGG[AT]
2427 TCCGGT
BstEII 8 GGTTACC G^GT[ACGT]ACC
2932 GGTCACC
BstPI 8 GGTTACC G^GT[ACGT]ACC
2932 GGTCACC
Bsu36I 3053 CCTGAGG CC^T[ACGT]AGG
5066 CCTTAGG
CelII 3155 GCTCAGC GC^T[ACGT]AGC
3610 GCTCAGC
Eco81I 3053 CCTGAGG CC^T[ACGT]AGG
5066 CCTTAGG
EcoNI 3053 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3233 CCTGATGAAGG
EcoO65I 8 GGTTACC G^GT[ACGT]ACC
2932 GGTCACC
KasI 38 GGCGCC G^GCGCC
323 GGCGCC
NaeI 88 GCCGGC GCC^GGC
320 GCCGGC
NarI 38 GGCGCC GG^CGCC
323 GGCGCC
NgoMIV 88 GCCGGC G^CCGGC
320 GCCGGC
SapI 2691 GAAGAGC GCTCTTC(1/4)
1977 GCTCTTC
SfoI 38 GGCGCC GGC^GCC
323 GGCGCC
XcmI 3573 CCAGAAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4623 CCAACAAGTAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII