##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccII AclI AflII AhdI Alw44I
Aor51HI ApaLI AscI AspEI AviII
AvrII BamHI BbeI BciVI BclI
BfrI BglI BglII BlnI BsiEI
BspDI BssHII Bst1107I BstEII BstPI
BstUI BstXI BstZ17I CfoI ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FbaI FseI FspI
HaeII HhaI HinP1I HpaI KasI
KspI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NotI NruI
PacI PmeI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SmaI
Sse8387I SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 748 GACGTC GACGT^C
Acc65I 4235 GGTACC G^GTACC
AccIII 2577 TCCGGA T^CCGGA
AcyI 748 GACGTC G[GA]^CG[CT]C
AgeI 1344 ACCGGT A^CCGGT
AocI 4494 CCTCAGG CC^T[ACGT]AGG
ApaI 547 GGGCCC GGGCC^C
AseI 4429 ATTAAT AT^TAAT
AsnI 4429 ATTAAT AT^TAAT
Asp718I 4235 GGTACC G^GTACC
AspHI 252 GAGCAC G[AT]GC[AT]^C
AspI 3974 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1646 CTCGAG C^[CT]CG[GA]G
BanI 4235 GGTACC G^G[CT][GA]CC
BbrPI 1779 CACGTG CAC^GTG
BbvCI 4230 GCTGAGG CCTCAGC(-5/-2)
BcnI 97 CCCGG CC^[GC]GG
BsaHI 748 GACGTC G[GA]^CG[CT]C
BseAI 2577 TCCGGA T^CCGGA
BsiHKAI 252 GAGCAC G[AT]GC[AT]^C
BsmBI 1399 CGTCTC CGTCTC(1/5)
BsmI 2164 GAATGC GAATGC(1/-1)
BsoBI 1646 CTCGAG C^[CT]CG[GA]G
BspEI 2577 TCCGGA T^CCGGA
BsrBI 1061 CCGCTC CCGCTC(-3/-3)
BsrFI 1344 ACCGGT [GA]^CCGG[CT]
Bsu36I 4494 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1344 ACCGGT [GA]^CCGG[CT]
DrdI 1767 GACGGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3529 CCATGG C^C[GA][CT]GG
Eco81I 4494 CCTCAGG CC^T[ACGT]AGG
EcoNI 2895 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3374 GATATC GAT^ATC
HgaI 1398 GCGTC GACGC(5/10)
Hin1I 748 GACGTC G[GA]^CG[CT]C
HincII 4705 GTTGAC GT[CT]^[GA]AC
HindII 4705 GTTGAC GT[CT]^[GA]AC
KpnI 4235 GGTACC GGTAC^C
MroI 2577 TCCGGA T^CCGGA
MspA1I 425 CAGCGG C[AC]G^C[GT]G
NciI 97 CCCGG CC^[GC]GG
NcoI 3529 CCATGG C^CATGG
NheI 2038 GCTAGC G^CTAGC
PaeR7I 1646 CTCGAG C^TCGAG
PflFI 3974 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1344 ACCGGT A^CCGGT
PmaCI 1779 CACGTG CAC^GTG
PmlI 1779 CACGTG CAC^GTG
PshBI 4429 ATTAAT AT^TAAT
SapI 1631 GAAGAGC GCTCTTC(1/4)
SgrAI 1343 CACCGGTG C[GA]^CCGG[CT]G
SpeI 4923 ACTAGT A^CTAGT
Tth111I 3974 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1646 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 3517 GTAGAC GT^[AC][GT]AC
3853 GTAGAC
AlwNI 43 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2850 CAGGGGCTG
BalI 449 TGGCCA TGG^CCA
647 TGGCCA
BlpI 3642 GCTGAGC GC^T[ACGT]AGC
4147 GCTAAGC
Bpu1102I 3642 GCTGAGC GC^T[ACGT]AGC
4147 GCTAAGC
BsaBI 336 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3596 GATCAAAATC
BsaWI 1344 ACCGGT [AT]^CCGG[AT]
2577 TCCGGA
BsiWI 853 CGTACG C^GTACG
3848 CGTACG
Bsp1407I 2130 TGTACA T^GTACA
2996 TGTACA
BspHI 3613 TCATGA T^CATGA
5051 TCATGA
BspLU11I 1412 ACATGT A^CATGT
3574 ACATGT
BspMI 4834 ACCTGC ACCTGC(4/8)
28 GCAGGT
BsrDI 2042 GCAATG GCAATG(2/0)
3236 GCAATG
BsrGI 2130 TGTACA T^GTACA
2996 TGTACA
BstBI 733 TTCGAA TT^CGAA
1069 TTCGAA
BstYI 2227 GGATCT [GA]^GATC[CT]
4988 GGATCT
CelII 3642 GCTGAGC GC^T[ACGT]AGC
4147 GCTAAGC
DraIII 95 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
3911 CACAAAGTG
EaeI 449 TGGCCA [CT]^GGCC[GA]
647 TGGCCA
EcoT14I 2159 CCTTGG C^C[AT][AT]GG
3529 CCATGG
HindIII 1214 AAGCTT A^AGCTT
3012 AAGCTT
MamI 336 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3596 GATCAAAATC
MflI 2227 GGATCT [GA]^GATC[CT]
4988 GGATCT
MluNI 449 TGGCCA TGG^CCA
647 TGGCCA
MscI 449 TGGCCA TGG^CCA
647 TGGCCA
NspV 733 TTCGAA TT^CGAA
1069 TTCGAA
PflMI 3175 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4880 CCATCTACTGG
PstI 2217 CTGCAG CTGCA^G
3267 CTGCAG
RcaI 3613 TCATGA T^CATGA
5051 TCATGA
SfuI 733 TTCGAA TT^CGAA
1069 TTCGAA
SnaBI 3846 TACGTA TAC^GTA
3850 TACGTA
SphI 2779 GCATGC GCATG^C
5026 GCATGC
SspBI 2130 TGTACA T^GTACA
2996 TGTACA
SspI 3019 AATATT AAT^ATT
4600 AATATT
StuI 4498 AGGCCT AGG^CCT
5017 AGGCCT
StyI 2159 CCTTGG C^C[AT][AT]GG
3529 CCATGG
Van91I 3175 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4880 CCATCTACTGG
XbaI 2680 TCTAGA T^CTAGA
4054 TCTAGA
XhoII 2227 GGATCT [GA]^GATC[CT]
4988 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AvaII BanII
BbsI BbvI BcgI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BssSI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRI EcoRII EcoT22I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI