##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BlnI BsaAI BsaHI BsaWI
BseAI BsiWI BsmBI BsoBI BspDI
BspEI BspMI BsrBI BssHII BssSI
BstBI BstUI BstXI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoT22I FseI FspI Hin1I
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Aor51HI 636 AGCGCT AGC^GCT
ApaI 443 GGGCCC GGGCC^C
BanI 2999 GGTGCC G^G[CT][GA]CC
BciVI 3629 GTATCC GTATCC(6/5)
BclI 3277 TGATCA T^GATCA
BglII 2648 AGATCT A^GATCT
BlpI 1095 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1095 GCTAAGC GC^T[ACGT]AGC
BsaBI 691 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1105 CTGCAC GTGCAG(16/14)
BsrFI 187 GCCGGT [GA]^CCGG[CT]
Bst1107I 5642 GTATAC GTA^TAC
BstEII 4032 GGTGACC G^GT[ACGT]ACC
BstPI 4032 GGTGACC G^GT[ACGT]ACC
BstZ17I 5642 GTATAC GTA^TAC
CelII 1095 GCTAAGC GC^T[ACGT]AGC
Cfr10I 187 GCCGGT [GA]^CCGG[CT]
DraIII 571 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 636 AGCGCT AGC^GCT
EcoO65I 4032 GGTGACC G^GT[ACGT]ACC
EcoRV 5002 GATATC GAT^ATC
FauI 115 GCGGG CCCGC(4/6)
FbaI 3277 TGATCA T^GATCA
HgaI 721 GACGC GACGC(5/10)
MamI 691 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3376 CAATTG C^AATTG
MunI 3376 CAATTG C^AATTG
PpuMI 1887 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 141 CGATCG CGAT^CG
SexAI 4359 ACCAGGT A^CC[AT]GGT
SphI 4087 GCATGC GCATG^C
XcmI 2267 CCATATGTTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2473 GTAGAC GT^[AC][GT]AC
5642 GTATAC
AclI 2510 AACGTT AA^CGTT
3674 AACGTT
AocI 245 CCTAAGG CC^T[ACGT]AGG
615 CCTGAGG
Asp700I 48 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1636 GAACACTTTC
BalI 433 TGGCCA TGG^CCA
4473 TGGCCA
BbsI 718 GAAGAC GAAGAC(2/6)
4095 GAAGAC
BcnI 128 CCGGG CC^[GC]GG
3841 CCCGG
BglI 1334 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4284 GCCATCAAGGC
BpmI 43 CTCCAG CTGGAG(16/14)
1167 CTGGAG
BpuAI 718 GAAGAC GAAGAC(2/6)
4095 GAAGAC
BsaI 1234 GAGACC GGTCTC(1/5)
2262 GGTCTC
BseRI 158 GAGGAG GAGGAG(10/8)
3963 GAGGAG
BsiEI 141 CGATCG CG[GA][CT]^CG
1325 CGACCG
Bsp1407I 3283 TGTACA T^GTACA
5612 TGTACA
BsrGI 3283 TGTACA T^GTACA
5612 TGTACA
Bsu36I 245 CCTAAGG CC^T[ACGT]AGG
615 CCTGAGG
EaeI 433 TGGCCA [CT]^GGCC[GA]
4473 TGGCCA
Eco81I 245 CCTAAGG CC^T[ACGT]AGG
615 CCTGAGG
EcoRI 4686 GAATTC G^AATTC
4993 GAATTC
HaeII 636 AGCGCT [GA]GCGC^[CT]
5320 AGCGCC
HincII 668 GTCAAC GT[CT]^[GA]AC
2844 GTCAAC
HindII 668 GTCAAC GT[CT]^[GA]AC
2844 GTCAAC
MluNI 433 TGGCCA TGG^CCA
4473 TGGCCA
MscI 433 TGGCCA TGG^CCA
4473 TGGCCA
NciI 128 CCGGG CC^[GC]GG
3841 CCCGG
NcoI 1520 CCATGG C^CATGG
5534 CCATGG
NdeI 1831 CATATG CA^TATG
2268 CATATG
PflMI 2365 CCATCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4050 CCAGGTGATGG
Psp1406I 2510 AACGTT AA^CGTT
3674 AACGTT
PstI 889 CTGCAG CTGCA^G
1610 CTGCAG
SapI 51 GCTCTTC GCTCTTC(1/4)
867 GCTCTTC
SpeI 2760 ACTAGT A^CTAGT
4985 ACTAGT
SspBI 3283 TGTACA T^GTACA
5612 TGTACA
Van91I 2365 CCATCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4050 CCAGGTGATGG
XmnI 48 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1636 GAACACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BanII BbvI
BfaI BfrI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII