##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI AscI Asp718I AviII AvrII
BbrPI BbvCI BciVI BfrI BglI
BlnI BsaBI BsmBI BsrBI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
Eco47III EcoNI EcoRV EcoT22I FseI
FspI KpnI MamI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 788 GTGCAC G^TGCAC
ApaLI 788 GTGCAC G^TGCAC
Asp700I 3700 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3480 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3002 GCACAGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3649 TGATCA T^GATCA
BglII 5064 AGATCT A^GATCT
BlpI 3568 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3568 GCTCAGC GC^T[ACGT]AGC
BsiEI 2385 CGGCCG CG[GA][CT]^CG
BsiWI 1020 CGTACG C^GTACG
BsmI 4502 GAATGC GAATGC(1/-1)
Bsp1407I 1691 TGTACA T^GTACA
BspDI 1902 ATCGAT AT^CGAT
BsrGI 1691 TGTACA T^GTACA
BstAPI 1557 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3255 GGTGACC G^GT[ACGT]ACC
BstPI 3255 GGTGACC G^GT[ACGT]ACC
CelII 3568 GCTCAGC GC^T[ACGT]AGC
ClaI 1902 ATCGAT AT^CGAT
CpoI 2908 CGGTCCG CG^G[AT]CCG
EagI 2385 CGGCCG C^GGCCG
EclXI 2385 CGGCCG C^GGCCG
Eco52I 2385 CGGCCG C^GGCCG
EcoO65I 3255 GGTGACC G^GT[ACGT]ACC
FbaI 3649 TGATCA T^GATCA
HpaI 2761 GTTAAC GTT^AAC
KspI 63 CCGCGG CCGC^GG
NheI 5016 GCTAGC G^CTAGC
PaeR7I 3236 CTCGAG C^TCGAG
PflFI 3480 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 2908 CGGTCCG CG^G[AT]CCG
SacII 63 CCGCGG CCGC^GG
SmaI 2106 CCCGGG CCC^GGG
SpeI 2370 ACTAGT A^CTAGT
SphI 2317 GCATGC GCATG^C
SspBI 1691 TGTACA T^GTACA
Tth111I 3480 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3236 CTCGAG C^TCGAG
XmaCI 2106 CCCGGG C^CCGGG
XmaI 2106 CCCGGG C^CCGGG
XmnI 3700 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 7 TCCGGA T^CCGGA
2151 TCCGGA
AhdI 1940 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4275 GACACTGCGTC
ApaI 4254 GGGCCC GGGCC^C
4261 GGGCCC
AspEI 1940 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4275 GACACTGCGTC
BalI 1122 TGGCCA TGG^CCA
4080 TGGCCA
BamHI 1252 GGATCC G^GATCC
3580 GGATCC
BbeI 2663 GGCGCC GGCGC^C
4203 GGCGCC
BsaAI 1118 TACGTG [CT]AC^GT[GA]
3787 CACGTA
BseAI 7 TCCGGA T^CCGGA
2151 TCCGGA
BspEI 7 TCCGGA T^CCGGA
2151 TCCGGA
BspLU11I 667 ACATGT A^CATGT
3799 ACATGT
BssSI 2634 CTCGTG CACGAG(-5/-1)
2801 CTCGTG
BstXI 74 CCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4534 CCATCAGCATGG
DraI 4909 TTTAAA TTT^AAA
5187 TTTAAA
DrdI 2780 GACTTTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3232 GACACTCGAGTC
Eam1105I 1940 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4275 GACACTGCGTC
HindIII 2344 AAGCTT A^AGCTT
3091 AAGCTT
KasI 2663 GGCGCC G^GCGCC
4203 GGCGCC
MluNI 1122 TGGCCA TGG^CCA
4080 TGGCCA
MroI 7 TCCGGA T^CCGGA
2151 TCCGGA
MscI 1122 TGGCCA TGG^CCA
4080 TGGCCA
NarI 2663 GGCGCC GG^CGCC
4203 GGCGCC
PflMI 3624 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5165 CCACACCGTGG
SacI 3775 GAGCTC GAGCT^C
4765 GAGCTC
SapI 5175 GAAGAGC GCTCTTC(1/4)
5030 GCTCTTC
SfoI 2663 GGCGCC GGC^GCC
4203 GGCGCC
StuI 2234 AGGCCT AGG^CCT
4236 AGGCCT
Van91I 3624 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5165 CCACACCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AseI AsnI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII