################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AccII AclI AgeI Alw44I Aor51HI ApaLI AscI Asp718I AvrII BbeI BglI BglII BlnI BsiWI BspHI BssHII BssSI BstAPI BstBI BstUI CpoI DraIII EagI EclXI Eco47III Eco52I FseI HpaI KasI KpnI KspI MluI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PflMI PinAI PmeI PshAI Psp1406I RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflII 3415 CTTAAG C^TTAAG AocI 3750 CCTGAGG CC^T[ACGT]AGG AseI 4030 ATTAAT AT^TAAT AsnI 4030 ATTAAT AT^TAAT AviII 3640 TGCGCA TGC^GCA BamHI 5591 GGATCC G^GATCC BanI 1900 GGCACC G^G[CT][GA]CC BbsI 3062 GTCTTC GAAGAC(2/6) BclI 821 TGATCA T^GATCA BfrI 3415 CTTAAG C^TTAAG BpuAI 3062 GTCTTC GAAGAC(2/6) BsaBI 3012 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspDI 307 ATCGAT AT^CGAT BspLU11I 4891 ACATGT A^CATGT BsrFI 3624 GCCGGT [GA]^CCGG[CT] Bst1107I 2906 GTATAC GTA^TAC BstEII 1782 GGTCACC G^GT[ACGT]ACC BstPI 1782 GGTCACC G^GT[ACGT]ACC BstZ17I 2906 GTATAC GTA^TAC Bsu36I 3750 CCTGAGG CC^T[ACGT]AGG Cfr10I 3624 GCCGGT [GA]^CCGG[CT] ClaI 307 ATCGAT AT^CGAT DrdI 3719 GACCGTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3750 CCTGAGG CC^T[ACGT]AGG EcoO65I 1782 GGTCACC G^GT[ACGT]ACC EcoRI 4741 GAATTC G^AATTC FbaI 821 TGATCA T^GATCA FspI 3640 TGCGCA TGC^GCA HaeII 3860 AGCGCC [GA]GCGC^[CT] HindIII 1214 AAGCTT A^AGCTT MamI 3012 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3969 CAATTG C^AATTG MunI 3969 CAATTG C^AATTG PaeR7I 1775 CTCGAG C^TCGAG PpuMI 3371 AGGTCCC [GA]G^G[AT]CC[CT] PshBI 4030 ATTAAT AT^TAAT SacI 2707 GAGCTC GAGCT^C XhoI 1775 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AccIII 2618 TCCGGA T^CCGGA 3173 TCCGGA AcyI 792 GGCGTC G[GA]^CG[CT]C 2221 GGCGTC ApaI 1875 GGGCCC GGGCC^C 2271 GGGCCC Asp700I 961 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1393 GAACACCTTC AspI 1028 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2203 GACCATGTC AvaI 1775 CTCGAG C^[CT]CG[GA]G 2268 CTCGGG BalI 510 TGGCCA TGG^CCA 2540 TGGCCA BbrPI 528 CACGTG CAC^GTG 2550 CACGTG BbvCI 3343 CCTCAGC CCTCAGC(-5/-2) 5582 CCTCAGC BcgI 3868 GCAGAGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5021 GCATGTATATCG BciVI 1518 GGATAC GTATCC(6/5) 540 GTATCC BlpI 2125 GCTGAGC GC^T[ACGT]AGC 5162 GCTCAGC Bpu1102I 2125 GCTGAGC GC^T[ACGT]AGC 5162 GCTCAGC BsaAI 528 CACGTG [CT]AC^GT[GA] 2550 CACGTG BsaHI 792 GGCGTC G[GA]^CG[CT]C 2221 GGCGTC BsaI 552 GGTCTC GGTCTC(1/5) 1633 GGTCTC BseAI 2618 TCCGGA T^CCGGA 3173 TCCGGA BsmI 1601 GAATGC GAATGC(1/-1) 3326 GAATGC BsoBI 1775 CTCGAG C^[CT]CG[GA]G 2268 CTCGGG BspEI 2618 TCCGGA T^CCGGA 3173 TCCGGA BsrBI 2784 CCGCTC CCGCTC(-3/-3) 1778 GAGCGG CelII 2125 GCTGAGC GC^T[ACGT]AGC 5162 GCTCAGC DraI 4157 TTTAAA TTT^AAA 5857 TTTAAA EcoNI 1911 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3746 CCTGCCTGAGG EcoT22I 780 ATGCAT ATGCA^T 5019 ATGCAT FauI 2783 CCCGC CCCGC(4/6) 2830 CCCGC Hin1I 792 GGCGTC G[GA]^CG[CT]C 2221 GGCGTC HincII 1003 GTCAAC GT[CT]^[GA]AC 5659 GTTGAC HindII 1003 GTCAAC GT[CT]^[GA]AC 5659 GTTGAC MluNI 510 TGGCCA TGG^CCA 2540 TGGCCA MroI 2618 TCCGGA T^CCGGA 3173 TCCGGA MscI 510 TGGCCA TGG^CCA 2540 TGGCCA NdeI 1654 CATATG CA^TATG 5699 CATATG NsiI 780 ATGCAT ATGCA^T 5019 ATGCAT PflFI 1028 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2203 GACCATGTC PmaCI 528 CACGTG CAC^GTG 2550 CACGTG PmlI 528 CACGTG CAC^GTG 2550 CACGTG PvuI 1590 CGATCG CGAT^CG 2165 CGATCG SmlI 1775 CTCGAG C^T[CT][GA]AG 3415 CTTAAG SphI 1704 GCATGC GCATG^C 3643 GCATGC Tth111I 1028 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2203 GACCATGTC XmnI 961 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1393 GAACACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI ApoI AspEI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII