##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AvrII BbeI BglI BglII BlnI
BsiWI BspHI BssHII BssSI BstAPI
BstBI BstUI CpoI DraIII EagI
EclXI Eco47III Eco52I FseI HpaI
KasI KpnI KspI MluI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PflMI PinAI PmeI
PshAI Psp1406I RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 3415 CTTAAG C^TTAAG
AocI 3750 CCTGAGG CC^T[ACGT]AGG
AseI 4030 ATTAAT AT^TAAT
AsnI 4030 ATTAAT AT^TAAT
AviII 3640 TGCGCA TGC^GCA
BamHI 5591 GGATCC G^GATCC
BanI 1900 GGCACC G^G[CT][GA]CC
BbsI 3062 GTCTTC GAAGAC(2/6)
BclI 821 TGATCA T^GATCA
BfrI 3415 CTTAAG C^TTAAG
BpuAI 3062 GTCTTC GAAGAC(2/6)
BsaBI 3012 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 307 ATCGAT AT^CGAT
BspLU11I 4891 ACATGT A^CATGT
BsrFI 3624 GCCGGT [GA]^CCGG[CT]
Bst1107I 2906 GTATAC GTA^TAC
BstEII 1782 GGTCACC G^GT[ACGT]ACC
BstPI 1782 GGTCACC G^GT[ACGT]ACC
BstZ17I 2906 GTATAC GTA^TAC
Bsu36I 3750 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3624 GCCGGT [GA]^CCGG[CT]
ClaI 307 ATCGAT AT^CGAT
DrdI 3719 GACCGTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3750 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1782 GGTCACC G^GT[ACGT]ACC
EcoRI 4741 GAATTC G^AATTC
FbaI 821 TGATCA T^GATCA
FspI 3640 TGCGCA TGC^GCA
HaeII 3860 AGCGCC [GA]GCGC^[CT]
HindIII 1214 AAGCTT A^AGCTT
MamI 3012 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3969 CAATTG C^AATTG
MunI 3969 CAATTG C^AATTG
PaeR7I 1775 CTCGAG C^TCGAG
PpuMI 3371 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 4030 ATTAAT AT^TAAT
SacI 2707 GAGCTC GAGCT^C
XhoI 1775 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 2618 TCCGGA T^CCGGA
3173 TCCGGA
AcyI 792 GGCGTC G[GA]^CG[CT]C
2221 GGCGTC
ApaI 1875 GGGCCC GGGCC^C
2271 GGGCCC
Asp700I 961 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1393 GAACACCTTC
AspI 1028 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2203 GACCATGTC
AvaI 1775 CTCGAG C^[CT]CG[GA]G
2268 CTCGGG
BalI 510 TGGCCA TGG^CCA
2540 TGGCCA
BbrPI 528 CACGTG CAC^GTG
2550 CACGTG
BbvCI 3343 CCTCAGC CCTCAGC(-5/-2)
5582 CCTCAGC
BcgI 3868 GCAGAGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5021 GCATGTATATCG
BciVI 1518 GGATAC GTATCC(6/5)
540 GTATCC
BlpI 2125 GCTGAGC GC^T[ACGT]AGC
5162 GCTCAGC
Bpu1102I 2125 GCTGAGC GC^T[ACGT]AGC
5162 GCTCAGC
BsaAI 528 CACGTG [CT]AC^GT[GA]
2550 CACGTG
BsaHI 792 GGCGTC G[GA]^CG[CT]C
2221 GGCGTC
BsaI 552 GGTCTC GGTCTC(1/5)
1633 GGTCTC
BseAI 2618 TCCGGA T^CCGGA
3173 TCCGGA
BsmI 1601 GAATGC GAATGC(1/-1)
3326 GAATGC
BsoBI 1775 CTCGAG C^[CT]CG[GA]G
2268 CTCGGG
BspEI 2618 TCCGGA T^CCGGA
3173 TCCGGA
BsrBI 2784 CCGCTC CCGCTC(-3/-3)
1778 GAGCGG
CelII 2125 GCTGAGC GC^T[ACGT]AGC
5162 GCTCAGC
DraI 4157 TTTAAA TTT^AAA
5857 TTTAAA
EcoNI 1911 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3746 CCTGCCTGAGG
EcoT22I 780 ATGCAT ATGCA^T
5019 ATGCAT
FauI 2783 CCCGC CCCGC(4/6)
2830 CCCGC
Hin1I 792 GGCGTC G[GA]^CG[CT]C
2221 GGCGTC
HincII 1003 GTCAAC GT[CT]^[GA]AC
5659 GTTGAC
HindII 1003 GTCAAC GT[CT]^[GA]AC
5659 GTTGAC
MluNI 510 TGGCCA TGG^CCA
2540 TGGCCA
MroI 2618 TCCGGA T^CCGGA
3173 TCCGGA
MscI 510 TGGCCA TGG^CCA
2540 TGGCCA
NdeI 1654 CATATG CA^TATG
5699 CATATG
NsiI 780 ATGCAT ATGCA^T
5019 ATGCAT
PflFI 1028 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2203 GACCATGTC
PmaCI 528 CACGTG CAC^GTG
2550 CACGTG
PmlI 528 CACGTG CAC^GTG
2550 CACGTG
PvuI 1590 CGATCG CGAT^CG
2165 CGATCG
SmlI 1775 CTCGAG C^T[CT][GA]AG
3415 CTTAAG
SphI 1704 GCATGC GCATG^C
3643 GCATGC
Tth111I 1028 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2203 GACCATGTC
XmnI 961 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1393 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI ApoI
AspEI AspHI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI EcoO109I EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NheI NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII