##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AgeI Alw44I Aor51HI ApaLI
AscI AviII AvrII BbeI BcgI
BclI BlnI BsiWI BspDI BsrBI
BssHII BstBI ClaI CpoI DraIII
DrdI Eco47III EcoNI EcoRI EcoRV
FbaI FseI FspI HaeII KasI
KspI MfeI MluI MunI NarI
NheI NotI NruI NspV PacI
PinAI PmeI Psp1406I RsrII SacII
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2929 GGTACC G^GTACC
AccII 3757 CGCG CG^CG
AccIII 2892 TCCGGA T^CCGGA
AflII 2264 CTTAAG C^TTAAG
ApaI 2769 GGGCCC GGGCC^C
AseI 1420 ATTAAT AT^TAAT
AsnI 1420 ATTAAT AT^TAAT
Asp718I 2929 GGTACC G^GTACC
BamHI 2401 GGATCC G^GATCC
BbrPI 629 CACGTG CAC^GTG
BbvCI 2739 CCTCAGC CCTCAGC(-5/-2)
BfrI 2264 CTTAAG C^TTAAG
BglI 534 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1087 AGATCT A^GATCT
BsaWI 2892 TCCGGA [AT]^CCGG[AT]
BseAI 2892 TCCGGA T^CCGGA
BspEI 2892 TCCGGA T^CCGGA
BspLU11I 3524 ACATGT A^CATGT
BssSI 383 CACGAG CACGAG(-5/-1)
Bst1107I 4270 GTATAC GTA^TAC
BstUI 3757 CGCG CG^CG
BstZ17I 4270 GTATAC GTA^TAC
EagI 1350 CGGCCG C^GGCCG
EclXI 1350 CGGCCG C^GGCCG
Eco52I 1350 CGGCCG C^GGCCG
EcoT22I 4458 ATGCAT ATGCA^T
HpaI 4496 GTTAAC GTT^AAC
KpnI 2929 GGTACC GGTAC^C
MroI 2892 TCCGGA T^CCGGA
MvnI 3757 CGCG CG^CG
NaeI 1348 GCCGGC GCC^GGC
NcoI 803 CCATGG C^CATGG
NdeI 1432 CATATG CA^TATG
NgoMIV 1348 GCCGGC G^CCGGC
NsiI 4458 ATGCAT ATGCA^T
PaeR7I 3799 CTCGAG C^TCGAG
PmaCI 629 CACGTG CAC^GTG
PmlI 629 CACGTG CAC^GTG
PshBI 1420 ATTAAT AT^TAAT
PvuI 1830 CGATCG CGAT^CG
SalI 3411 GTCGAC G^TCGAC
ScaI 5203 AGTACT AGT^ACT
SnaBI 4621 TACGTA TAC^GTA
SphI 1515 GCATGC GCATG^C
StuI 3795 AGGCCT AGG^CCT
XhoI 3799 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 492 GACGTC GACGT^C
3391 GACGTC
AlwNI 2336 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
2813 CAGGGTCTG
AocI 2341 CCTGAGG CC^T[ACGT]AGG
3004 CCTGAGG
AspI 226 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACAGGGTC
BsaBI 567 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
919 GATAGACATC
BsiEI 1350 CGGCCG CG[GA][CT]^CG
1830 CGATCG
Bsp1407I 849 TGTACA T^GTACA
2251 TGTACA
BsrDI 445 CATTGC GCAATG(2/0)
2922 CATTGC
BsrFI 1348 GCCGGC [GA]^CCGG[CT]
3140 ACCGGC
BsrGI 849 TGTACA T^GTACA
2251 TGTACA
BstAPI 1180 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3877 GCAAGGGTTGC
BstEII 763 GGTCACC G^GT[ACGT]ACC
2729 GGTCACC
BstPI 763 GGTCACC G^GT[ACGT]ACC
2729 GGTCACC
BstXI 3821 CCAGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3893 CCAGCACCTTGG
Bsu36I 2341 CCTGAGG CC^T[ACGT]AGG
3004 CCTGAGG
CfoI 3756 GCGC GCG^C
5287 GCGC
Cfr10I 1348 GCCGGC [GA]^CCGG[CT]
3140 ACCGGC
DsaI 803 CCATGG C^C[GA][CT]GG
2686 CCGTGG
Eco81I 2341 CCTGAGG CC^T[ACGT]AGG
3004 CCTGAGG
EcoO65I 763 GGTCACC G^GT[ACGT]ACC
2729 GGTCACC
FauI 1394 CCCGC CCCGC(4/6)
3514 CCCGC
HhaI 3756 GCGC GCG^C
5287 GCGC
HinP1I 3756 GCGC G^CGC
5287 GCGC
MamI 567 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
919 GATAGACATC
PflFI 226 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACAGGGTC
PflMI 65 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
240 CCACCTGCTGG
PshAI 189 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2174 GACTGCTGTC
PstI 2203 CTGCAG CTGCA^G
4348 CTGCAG
SacI 5032 GAGCTC GAGCT^C
5381 GAGCTC
SpeI 2119 ACTAGT A^CTAGT
2368 ACTAGT
SspBI 849 TGTACA T^GTACA
2251 TGTACA
Tth111I 226 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACAGGGTC
Van91I 65 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
240 CCACCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI ApoI
Asp700I AspEI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII EaeI Eam1105I EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI Hin1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI