##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI ApaI AscI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbeI BcgI BfrI BglI
BglII BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrFI
BssHII BstBI Cfr10I ClaI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
Psp1406I PvuI SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 1895 GACGCC G[GA]^CG[CT]C
AflIII 3304 ACACGT A^C[GA][CT]GT
AseI 4498 ATTAAT AT^TAAT
AsnI 4498 ATTAAT AT^TAAT
AspI 1028 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 6 CCCGGG C^[CT]CG[GA]G
BanI 4988 GGCACC G^G[CT][GA]CC
BbvCI 3824 CCTCAGC CCTCAGC(-5/-2)
BciVI 1691 GGATAC GTATCC(6/5)
BclI 1448 TGATCA T^GATCA
BlpI 54 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 54 GCTGAGC GC^T[ACGT]AGC
BsaHI 1895 GACGCC G[GA]^CG[CT]C
BsoBI 6 CCCGGG C^[CT]CG[GA]G
BspHI 2906 TCATGA T^CATGA
BsrBI 4419 GAGCGG CCGCTC(-3/-3)
BstEII 4718 GGTAACC G^GT[ACGT]ACC
BstPI 4718 GGTAACC G^GT[ACGT]ACC
BstYI 772 AGATCC [GA]^GATC[CT]
CelII 54 GCTGAGC GC^T[ACGT]AGC
CpoI 430 CGGACCG CG^G[AT]CCG
DraIII 153 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1895 GACGCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4677 CCATGG C^C[GA][CT]GG
EaeI 1740 CGGCCA [CT]^GGCC[GA]
EcoO65I 4718 GGTAACC G^GT[ACGT]ACC
FbaI 1448 TGATCA T^GATCA
Hin1I 1895 GACGCC G[GA]^CG[CT]C
HincII 4735 GTCAAC GT[CT]^[GA]AC
HindII 4735 GTCAAC GT[CT]^[GA]AC
HindIII 2025 AAGCTT A^AGCTT
MflI 772 AGATCC [GA]^GATC[CT]
NcoI 4677 CCATGG C^CATGG
NdeI 2950 CATATG CA^TATG
PflFI 1028 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4498 ATTAAT AT^TAAT
RcaI 2906 TCATGA T^CATGA
RsrII 430 CGGACCG CG^G[AT]CCG
SapI 878 GAAGAGC GCTCTTC(1/4)
ScaI 4322 AGTACT AGT^ACT
SmaI 6 CCCGGG CCC^GGG
SphI 2677 GCATGC GCATG^C
Tth111I 1028 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 772 AGATCC [GA]^GATC[CT]
XmaCI 6 CCCGGG C^CCGGG
XmaI 6 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 237 GTGCAC G^TGCAC
2810 GTGCAC
AocI 242 CCTTAGG CC^T[ACGT]AGG
1713 CCTCAGG
Aor51HI 682 AGCGCT AGC^GCT
4910 AGCGCT
ApaLI 237 GTGCAC G^TGCAC
2810 GTGCAC
BbrPI 153 CACGTG CAC^GTG
3305 CACGTG
BsaAI 153 CACGTG [CT]AC^GT[GA]
3305 CACGTG
BsaBI 2534 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4622 GATTTCCATC
BsmI 2072 GAATGC GAATGC(1/-1)
2604 GAATGC
BssSI 1816 CTCGTG CACGAG(-5/-1)
2994 CTCGTG
BstAPI 399 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2448 GCAGGAGATGC
BstXI 519 CCAGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1142 CCATAATCCTGG
Bsu36I 242 CCTTAGG CC^T[ACGT]AGG
1713 CCTCAGG
Eco47III 682 AGCGCT AGC^GCT
4910 AGCGCT
Eco81I 242 CCTTAGG CC^T[ACGT]AGG
1713 CCTCAGG
EcoNI 692 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3154 CCTGTAAGAGG
EcoRI 647 GAATTC G^AATTC
2723 GAATTC
HaeII 682 AGCGCT [GA]GCGC^[CT]
4910 AGCGCT
HapII 7 CCGG C^CGG
2180 CCGG
HpaII 7 CCGG C^CGG
2180 CCGG
MamI 2534 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4622 GATTTCCATC
MspI 7 CCGG C^CGG
2180 CCGG
NheI 2355 GCTAGC G^CTAGC
2681 GCTAGC
PmaCI 153 CACGTG CAC^GTG
3305 CACGTG
PmlI 153 CACGTG CAC^GTG
3305 CACGTG
SmlI 2752 CTTGAG C^T[CT][GA]AG
4007 CTCAAG
SpeI 2970 ACTAGT A^CTAGT
3660 ACTAGT
TaqI 633 TCGA T^CGA
1709 TCGA
TthHB8I 633 TCGA T^CGA
1709 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI Bst1107I BstF5I BstNI BstUI
BstZ17I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI SspI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI