##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccII AclI AgeI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbeI BbvCI BsiEI BsiWI
BspDI BssHII Bst1107I BstBI BstUI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoRV FseI
FspI HgaI HpaI KasI KpnI
KspI MluI MvnI NarI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 2243 GACGTC GACGT^C
AccIII 2605 TCCGGA T^CCGGA
AcyI 2243 GACGTC G[GA]^CG[CT]C
AflII 5401 CTTAAG C^TTAAG
AhdI 4341 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2336 AGCGCT AGC^GCT
ApaI 1713 GGGCCC GGGCC^C
AspEI 4341 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3985 CCTAGG C^CTAGG
BbrPI 3011 CACGTG CAC^GTG
BcgI 2203 CGAGCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 691 GGATAC GTATCC(6/5)
BfrI 5401 CTTAAG C^TTAAG
BglII 19 AGATCT A^GATCT
BlnI 3985 CCTAGG C^CTAGG
BlpI 5687 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5687 GCTGAGC GC^T[ACGT]AGC
BsaAI 3011 CACGTG [CT]AC^GT[GA]
BsaHI 2243 GACGTC G[GA]^CG[CT]C
BseAI 2605 TCCGGA T^CCGGA
BsmBI 1119 GAGACG CGTCTC(1/5)
BsmI 422 GAATGC GAATGC(1/-1)
BspEI 2605 TCCGGA T^CCGGA
BspLU11I 4673 ACATGT A^CATGT
BsrBI 3817 GAGCGG CCGCTC(-3/-3)
BsrDI 4624 CATTGC GCAATG(2/0)
BstEII 3413 GGTCACC G^GT[ACGT]ACC
BstPI 3413 GGTCACC G^GT[ACGT]ACC
CelII 5687 GCTGAGC GC^T[ACGT]AGC
CfoI 2337 GCGC GCG^C
Eam1105I 4341 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2336 AGCGCT AGC^GCT
EcoNI 2318 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3413 GGTCACC G^GT[ACGT]ACC
EcoT22I 5516 ATGCAT ATGCA^T
HaeII 2336 AGCGCT [GA]GCGC^[CT]
HhaI 2337 GCGC GCG^C
Hin1I 2243 GACGTC G[GA]^CG[CT]C
HinP1I 2337 GCGC G^CGC
HincII 545 GTCAAC GT[CT]^[GA]AC
HindII 545 GTCAAC GT[CT]^[GA]AC
HindIII 1821 AAGCTT A^AGCTT
MroI 2605 TCCGGA T^CCGGA
NaeI 3918 GCCGGC GCC^GGC
NgoMIV 3918 GCCGGC G^CCGGC
NsiI 5516 ATGCAT ATGCA^T
PaeR7I 2508 CTCGAG C^TCGAG
PmaCI 3011 CACGTG CAC^GTG
PmlI 3011 CACGTG CAC^GTG
PpuMI 2860 AGGACCC [GA]G^G[AT]CC[CT]
SacI 2886 GAGCTC GAGCT^C
ScaI 4426 AGTACT AGT^ACT
SmaI 4578 CCCGGG CCC^GGG
StuI 5596 AGGCCT AGG^CCT
XhoI 2508 CTCGAG C^TCGAG
XmaCI 4578 CCCGGG C^CCGGG
XmaI 4578 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 3012 ACGTGT A^C[GA][CT]GT
4673 ACATGT
AocI 614 CCTGAGG CC^T[ACGT]AGG
4792 CCTGAGG
BglI 1582 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3328 GCCAGCACGGC
BsaBI 922 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2914 GATGTTCATC
BsaWI 86 TCCGGT [AT]^CCGG[AT]
2605 TCCGGA
Bsp1407I 1420 TGTACA T^GTACA
3561 TGTACA
BsrGI 1420 TGTACA T^GTACA
3561 TGTACA
BssSI 154 CTCGTG CACGAG(-5/-1)
4613 CTCGTG
Bsu36I 614 CCTGAGG CC^T[ACGT]AGG
4792 CCTGAGG
Eco81I 614 CCTGAGG CC^T[ACGT]AGG
4792 CCTGAGG
FauI 3725 CCCGC CCCGC(4/6)
1923 GCGGG
MamI 922 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2914 GATGTTCATC
MfeI 4041 CAATTG C^AATTG
5773 CAATTG
MunI 4041 CAATTG C^AATTG
5773 CAATTG
NdeI 1677 CATATG CA^TATG
3780 CATATG
PflMI 229 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4723 CCATTATTTGG
PshAI 2498 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5416 GACTTCAGTC
PstI 3101 CTGCAG CTGCA^G
4185 CTGCAG
SapI 3044 GAAGAGC GCTCTTC(1/4)
4659 GCTCTTC
SpeI 4907 ACTAGT A^CTAGT
5712 ACTAGT
SspBI 1420 TGTACA T^GTACA
3561 TGTACA
SspI 704 AATATT AAT^ATT
5152 AATATT
Van91I 229 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4723 CCATTATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI