################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AccII AclI AgeI AscI AseI AsnI Asp718I AspI AviII BamHI BbeI BbvCI BsiEI BsiWI BspDI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoRV FseI FspI HgaI HpaI KasI KpnI KspI MluI MvnI NarI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 2243 GACGTC GACGT^C AccIII 2605 TCCGGA T^CCGGA AcyI 2243 GACGTC G[GA]^CG[CT]C AflII 5401 CTTAAG C^TTAAG AhdI 4341 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 2336 AGCGCT AGC^GCT ApaI 1713 GGGCCC GGGCC^C AspEI 4341 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3985 CCTAGG C^CTAGG BbrPI 3011 CACGTG CAC^GTG BcgI 2203 CGAGCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 691 GGATAC GTATCC(6/5) BfrI 5401 CTTAAG C^TTAAG BglII 19 AGATCT A^GATCT BlnI 3985 CCTAGG C^CTAGG BlpI 5687 GCTGAGC GC^T[ACGT]AGC Bpu1102I 5687 GCTGAGC GC^T[ACGT]AGC BsaAI 3011 CACGTG [CT]AC^GT[GA] BsaHI 2243 GACGTC G[GA]^CG[CT]C BseAI 2605 TCCGGA T^CCGGA BsmBI 1119 GAGACG CGTCTC(1/5) BsmI 422 GAATGC GAATGC(1/-1) BspEI 2605 TCCGGA T^CCGGA BspLU11I 4673 ACATGT A^CATGT BsrBI 3817 GAGCGG CCGCTC(-3/-3) BsrDI 4624 CATTGC GCAATG(2/0) BstEII 3413 GGTCACC G^GT[ACGT]ACC BstPI 3413 GGTCACC G^GT[ACGT]ACC CelII 5687 GCTGAGC GC^T[ACGT]AGC CfoI 2337 GCGC GCG^C Eam1105I 4341 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2336 AGCGCT AGC^GCT EcoNI 2318 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3413 GGTCACC G^GT[ACGT]ACC EcoT22I 5516 ATGCAT ATGCA^T HaeII 2336 AGCGCT [GA]GCGC^[CT] HhaI 2337 GCGC GCG^C Hin1I 2243 GACGTC G[GA]^CG[CT]C HinP1I 2337 GCGC G^CGC HincII 545 GTCAAC GT[CT]^[GA]AC HindII 545 GTCAAC GT[CT]^[GA]AC HindIII 1821 AAGCTT A^AGCTT MroI 2605 TCCGGA T^CCGGA NaeI 3918 GCCGGC GCC^GGC NgoMIV 3918 GCCGGC G^CCGGC NsiI 5516 ATGCAT ATGCA^T PaeR7I 2508 CTCGAG C^TCGAG PmaCI 3011 CACGTG CAC^GTG PmlI 3011 CACGTG CAC^GTG PpuMI 2860 AGGACCC [GA]G^G[AT]CC[CT] SacI 2886 GAGCTC GAGCT^C ScaI 4426 AGTACT AGT^ACT SmaI 4578 CCCGGG CCC^GGG StuI 5596 AGGCCT AGG^CCT XhoI 2508 CTCGAG C^TCGAG XmaCI 4578 CCCGGG C^CCGGG XmaI 4578 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 3012 ACGTGT A^C[GA][CT]GT 4673 ACATGT AocI 614 CCTGAGG CC^T[ACGT]AGG 4792 CCTGAGG BglI 1582 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3328 GCCAGCACGGC BsaBI 922 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2914 GATGTTCATC BsaWI 86 TCCGGT [AT]^CCGG[AT] 2605 TCCGGA Bsp1407I 1420 TGTACA T^GTACA 3561 TGTACA BsrGI 1420 TGTACA T^GTACA 3561 TGTACA BssSI 154 CTCGTG CACGAG(-5/-1) 4613 CTCGTG Bsu36I 614 CCTGAGG CC^T[ACGT]AGG 4792 CCTGAGG Eco81I 614 CCTGAGG CC^T[ACGT]AGG 4792 CCTGAGG FauI 3725 CCCGC CCCGC(4/6) 1923 GCGGG MamI 922 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2914 GATGTTCATC MfeI 4041 CAATTG C^AATTG 5773 CAATTG MunI 4041 CAATTG C^AATTG 5773 CAATTG NdeI 1677 CATATG CA^TATG 3780 CATATG PflMI 229 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4723 CCATTATTTGG PshAI 2498 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5416 GACTTCAGTC PstI 3101 CTGCAG CTGCA^G 4185 CTGCAG SapI 3044 GAAGAGC GCTCTTC(1/4) 4659 GCTCTTC SpeI 4907 ACTAGT A^CTAGT 5712 ACTAGT SspBI 1420 TGTACA T^GTACA 3561 TGTACA SspI 704 AATATT AAT^ATT 5152 AATATT Van91I 229 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4723 CCATTATTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI