##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
ApaI AscI AseI AsnI AviII
BbeI BbrPI BcgI BclI BglI
BsaAI BsaHI BsiEI BsiWI BsmBI
BspDI BssHII BstBI BstUI ClaI
CpoI DraI EagI EclXI Eco52I
EcoNI EcoRI EcoRV FbaI FseI
FspI HgaI Hin1I KasI KspI
MluI MvnI NaeI NarI NgoMIV
NotI NruI NspV PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2648 GGTACC G^GTACC
AccIII 1447 TCCGGA T^CCGGA
AflII 38 CTTAAG C^TTAAG
Aor51HI 1549 AGCGCT AGC^GCT
Asp718I 2648 GGTACC G^GTACC
BalI 364 TGGCCA TGG^CCA
BfrI 38 CTTAAG C^TTAAG
BsaWI 1447 TCCGGA [AT]^CCGG[AT]
BseAI 1447 TCCGGA T^CCGGA
BsgI 3093 GTGCAG GTGCAG(16/14)
Bsp1407I 1852 TGTACA T^GTACA
BspEI 1447 TCCGGA T^CCGGA
BspMI 3107 GCAGGT ACCTGC(4/8)
BsrFI 941 ACCGGC [GA]^CCGG[CT]
BsrGI 1852 TGTACA T^GTACA
BssSI 3650 CTCGTG CACGAG(-5/-1)
Bst1107I 872 GTATAC GTA^TAC
BstAPI 3098 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1150 GGTCACC G^GT[ACGT]ACC
BstPI 1150 GGTCACC G^GT[ACGT]ACC
BstZ17I 872 GTATAC GTA^TAC
Cfr10I 941 ACCGGC [GA]^CCGG[CT]
Eco47III 1549 AGCGCT AGC^GCT
EcoO65I 1150 GGTCACC G^GT[ACGT]ACC
EcoT22I 4805 ATGCAT ATGCA^T
KpnI 2648 GGTACC GGTAC^C
MaeII 427 ACGT A^CGT
MfeI 3059 CAATTG C^AATTG
MluNI 364 TGGCCA TGG^CCA
MroI 1447 TCCGGA T^CCGGA
MscI 364 TGGCCA TGG^CCA
MunI 3059 CAATTG C^AATTG
NdeI 1609 CATATG CA^TATG
NsiI 4805 ATGCAT ATGCA^T
PacI 5103 TTAATTAA TTAAT^TAA
PaeR7I 664 CTCGAG C^TCGAG
PflMI 4622 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2181 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1037 GAAGAGC GCTCTTC(1/4)
ScaI 3259 AGTACT AGT^ACT
SpeI 3720 ACTAGT A^CTAGT
SphI 172 GCATGC GCATG^C
SspBI 1852 TGTACA T^GTACA
Van91I 4622 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 664 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 872 GTATAC GT^[AC][GT]AC
4758 GTAGAC
AflIII 3073 ACATGT A^C[GA][CT]GT
3569 ACATGT
AhdI 3494 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4655 GACCCCAAGTC
AspEI 3494 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4655 GACCCCAAGTC
AspI 4001 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5068 GACACAGTC
AvrII 2076 CCTAGG C^CTAGG
4352 CCTAGG
BamHI 697 GGATCC G^GATCC
1604 GGATCC
BbvCI 781 CCTCAGC CCTCAGC(-5/-2)
2779 GCTGAGG
BciVI 4412 GGATAC GTATCC(6/5)
3151 GTATCC
BlnI 2076 CCTAGG C^CTAGG
4352 CCTAGG
BlpI 1302 GCTGAGC GC^T[ACGT]AGC
2105 GCTGAGC
Bpu1102I 1302 GCTGAGC GC^T[ACGT]AGC
2105 GCTGAGC
BsaBI 378 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2839 GATGTAGATC
BspHI 2869 TCATGA T^CATGA
4811 TCATGA
BspLU11I 3073 ACATGT A^CATGT
3569 ACATGT
BsrBI 2752 CCGCTC CCGCTC(-3/-3)
1290 GAGCGG
BsrDI 1860 CATTGC GCAATG(2/0)
792 GCAATG
BstXI 233 CCAGACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3365 CCAGGAAGATGG
CelII 1302 GCTGAGC GC^T[ACGT]AGC
2105 GCTGAGC
DrdI 159 GACCTGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1142 GACATATCGGTC
Eam1105I 3494 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4655 GACCCCAAGTC
HaeII 1549 AGCGCT [GA]GCGC^[CT]
4168 AGCGCC
HpaI 1473 GTTAAC GTT^AAC
2792 GTTAAC
MamI 378 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2839 GATGTAGATC
PflFI 4001 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5068 GACACAGTC
RcaI 2869 TCATGA T^CATGA
4811 TCATGA
Tth111I 4001 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5068 GACACAGTC
XcmI 3634 CCAAGAACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4295 CCAGTTCTATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NheI NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI