##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
ApaI ApaLI AscI AspEI AviII
AvrII BbeI BclI BlnI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRV FbaI
FseI FspI HpaI KasI MluI
MroI NarI NcoI NotI NruI
NspV PacI PflMI PinAI PmeI
PvuI RsrII SalI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI SwaI Van91I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 329 GGTACC G^GTACC
AcyI 5202 GGCGTC G[GA]^CG[CT]C
AflII 782 CTTAAG C^TTAAG
AocI 4039 CCTAAGG CC^T[ACGT]AGG
AseI 1162 ATTAAT AT^TAAT
AsnI 1162 ATTAAT AT^TAAT
Asp718I 329 GGTACC G^GTACC
BamHI 1525 GGATCC G^GATCC
BbvCI 1261 CCTCAGC CCTCAGC(-5/-2)
BcgI 881 GCAAAAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 782 CTTAAG C^TTAAG
BglI 1329 GCCAGCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3723 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3723 GCTCAGC GC^T[ACGT]AGC
BsaBI 3684 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5202 GGCGTC G[GA]^CG[CT]C
BspLU11I 4883 ACATGT A^CATGT
BspMI 3747 ACCTGC ACCTGC(4/8)
BsrBI 259 CCGCTC CCGCTC(-3/-3)
Bsu36I 4039 CCTAAGG CC^T[ACGT]AGG
CelII 3723 GCTCAGC GC^T[ACGT]AGC
Eco81I 4039 CCTAAGG CC^T[ACGT]AGG
EcoRI 2924 GAATTC G^AATTC
HgaI 5203 GCGTC GACGC(5/10)
Hin1I 5202 GGCGTC G[GA]^CG[CT]C
KpnI 329 GGTACC GGTAC^C
KspI 162 CCGCGG CCGC^GG
MamI 3684 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2234 CAATTG C^AATTG
MunI 2234 CAATTG C^AATTG
NaeI 1452 GCCGGC GCC^GGC
NdeI 3297 CATATG CA^TATG
NgoMIV 1452 GCCGGC G^CCGGC
NheI 3562 GCTAGC G^CTAGC
PshAI 2059 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1162 ATTAAT AT^TAAT
SacII 162 CCGCGG CCGC^GG
SapI 4033 GCTCTTC GCTCTTC(1/4)
SbfI 3778 CCTGCAGG CCTGCA^GG
Sse8387I 3778 CCTGCAGG CCTGCA^GG
SspI 5029 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Aor51HI 2574 AGCGCT AGC^GCT
5254 AGCGCT
AspI 1691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3605 GACATTGTC
AvaI 1842 CTCGAG C^[CT]CG[GA]G
4291 CTCGAG
BciVI 4668 GGATAC GTATCC(6/5)
2770 GTATCC
BcnI 334 CCCGG CC^[GC]GG
5199 CCGGG
BsaI 3452 GAGACC GGTCTC(1/5)
1430 GGTCTC
BsoBI 1842 CTCGAG C^[CT]CG[GA]G
4291 CTCGAG
BsrDI 4758 CATTGC GCAATG(2/0)
5133 CATTGC
BsrFI 1452 GCCGGC [GA]^CCGG[CT]
3442 GCCGGT
BssSI 1199 CACGAG CACGAG(-5/-1)
4706 CTCGTG
BstEII 2193 GGTCACC G^GT[ACGT]ACC
4455 GGTTACC
BstPI 2193 GGTCACC G^GT[ACGT]ACC
4455 GGTTACC
BstXI 1020 CCAAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1419 CCATCGTGATGG
Cfr10I 1452 GCCGGC [GA]^CCGG[CT]
3442 GCCGGT
DraI 2978 TTTAAA TTT^AAA
5467 TTTAAA
DraIII 3030 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4466 CACATGGTG
DsaI 162 CCGCGG C^C[GA][CT]GG
273 CCACGG
Eco47III 2574 AGCGCT AGC^GCT
5254 AGCGCT
EcoNI 88 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
373 CCTGGCAGAGG
EcoO65I 2193 GGTCACC G^GT[ACGT]ACC
4455 GGTTACC
NciI 334 CCCGG CC^[GC]GG
5199 CCGGG
PaeR7I 1842 CTCGAG C^TCGAG
4291 CTCGAG
PflFI 1691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3605 GACATTGTC
SexAI 3180 ACCTGGT A^CC[AT]GGT
3918 ACCTGGT
SpeI 2415 ACTAGT A^CTAGT
3019 ACTAGT
StuI 1901 AGGCCT AGG^CCT
2295 AGGCCT
Tth111I 1691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3605 GACATTGTC
XhoI 1842 CTCGAG C^TCGAG
4291 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AclI AcsI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PmaCI PmlI PpuMI Psp1406I PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI