################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI BbeI BcgI BfrI BseAI BsiWI BspEI BssHII Bst1107I BstZ17I Bsu36I CpoI Eam1105I Eco47III Eco81I EcoRV HaeII HincII HindII HindIII HpaI KasI KpnI KspI MluI MroI NarI NotI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SnaBI Sse8387I SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 2665 GTCTAC GT^[AC][GT]AC AcyI 1223 GACGCC G[GA]^CG[CT]C Alw44I 2599 GTGCAC G^TGCAC ApaLI 2599 GTGCAC G^TGCAC AviII 612 TGCGCA TGC^GCA BamHI 1492 GGATCC G^GATCC BbrPI 2190 CACGTG CAC^GTG BclI 1536 TGATCA T^GATCA BlpI 4138 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4138 GCTCAGC GC^T[ACGT]AGC BsaBI 3878 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1223 GACGCC G[GA]^CG[CT]C BsaWI 1212 ACCGGA [AT]^CCGG[AT] BsiEI 1249 CGGCCG CG[GA][CT]^CG Bsp1407I 1236 TGTACA T^GTACA BsrFI 1247 GCCGGC [GA]^CCGG[CT] BsrGI 1236 TGTACA T^GTACA BstBI 861 TTCGAA TT^CGAA CelII 4138 GCTCAGC GC^T[ACGT]AGC Cfr10I 1247 GCCGGC [GA]^CCGG[CT] DraI 3192 TTTAAA TTT^AAA DraIII 2849 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1249 CGGCCG C^GGCCG EclXI 1249 CGGCCG C^GGCCG Eco52I 1249 CGGCCG C^GGCCG EcoRI 3737 GAATTC G^AATTC FbaI 1536 TGATCA T^GATCA FseI 1246 GGCCGGCC GGCCGG^CC FspI 612 TGCGCA TGC^GCA Hin1I 1223 GACGCC G[GA]^CG[CT]C MamI 3878 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1053 CAATTG C^AATTG MunI 1053 CAATTG C^AATTG NaeI 1247 GCCGGC GCC^GGC NgoMIV 1247 GCCGGC G^CCGGC NheI 3322 GCTAGC G^CTAGC NruI 4485 TCGCGA TCG^CGA NspV 861 TTCGAA TT^CGAA PmaCI 2190 CACGTG CAC^GTG PmlI 2190 CACGTG CAC^GTG PshAI 124 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1561 GAAGAGC GCTCTTC(1/4) SfuI 861 TTCGAA TT^CGAA SphI 1816 GCATGC GCATG^C SspBI 1236 TGTACA T^GTACA SspI 47 AATATT AAT^ATT XbaI 1656 TCTAGA T^CTAGA XcmI 5875 CCAGTGGTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AspI 4262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4654 GACCTTGTC AvrII 2531 CCTAGG C^CTAGG 3214 CCTAGG BanI 1070 GGTGCC G^G[CT][GA]CC 5554 GGTGCC BciVI 5533 GGATAC GTATCC(6/5) 3790 GTATCC BglI 299 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4198 GCCCGTGGGGC BglII 1105 AGATCT A^GATCT 2638 AGATCT BlnI 2531 CCTAGG C^CTAGG 3214 CCTAGG BsaAI 1905 TACGTG [CT]AC^GT[GA] 2190 CACGTG BsgI 4994 CTGCAC GTGCAG(16/14) 4460 GTGCAG BsrBI 2120 CCGCTC CCGCTC(-3/-3) 5558 CCGCTC BssSI 1293 CACGAG CACGAG(-5/-1) 1143 CTCGTG BstEII 4021 GGTAACC G^GT[ACGT]ACC 5738 GGTTACC BstPI 4021 GGTAACC G^GT[ACGT]ACC 5738 GGTTACC DrdI 1752 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3642 GACCCCCCTGTC EcoO65I 4021 GGTAACC G^GT[ACGT]ACC 5738 GGTTACC NdeI 3688 CATATG CA^TATG 3819 CATATG PflFI 4262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4654 GACCTTGTC SmaI 222 CCCGGG CCC^GGG 5658 CCCGGG SpeI 787 ACTAGT A^CTAGT 1734 ACTAGT Tth111I 4262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4654 GACCTTGTC XmaCI 222 CCCGGG C^CCGGG 5658 CCCGGG XmaI 222 CCCGGG C^CCGGG 5658 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspHI BspLU11I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I ClaI DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII