##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
BbeI BcgI BfrI BseAI BsiWI
BspEI BssHII Bst1107I BstZ17I Bsu36I
CpoI Eam1105I Eco47III Eco81I EcoRV
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MluI MroI
NarI NotI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI Sse8387I SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 2665 GTCTAC GT^[AC][GT]AC
AcyI 1223 GACGCC G[GA]^CG[CT]C
Alw44I 2599 GTGCAC G^TGCAC
ApaLI 2599 GTGCAC G^TGCAC
AviII 612 TGCGCA TGC^GCA
BamHI 1492 GGATCC G^GATCC
BbrPI 2190 CACGTG CAC^GTG
BclI 1536 TGATCA T^GATCA
BlpI 4138 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4138 GCTCAGC GC^T[ACGT]AGC
BsaBI 3878 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1223 GACGCC G[GA]^CG[CT]C
BsaWI 1212 ACCGGA [AT]^CCGG[AT]
BsiEI 1249 CGGCCG CG[GA][CT]^CG
Bsp1407I 1236 TGTACA T^GTACA
BsrFI 1247 GCCGGC [GA]^CCGG[CT]
BsrGI 1236 TGTACA T^GTACA
BstBI 861 TTCGAA TT^CGAA
CelII 4138 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1247 GCCGGC [GA]^CCGG[CT]
DraI 3192 TTTAAA TTT^AAA
DraIII 2849 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1249 CGGCCG C^GGCCG
EclXI 1249 CGGCCG C^GGCCG
Eco52I 1249 CGGCCG C^GGCCG
EcoRI 3737 GAATTC G^AATTC
FbaI 1536 TGATCA T^GATCA
FseI 1246 GGCCGGCC GGCCGG^CC
FspI 612 TGCGCA TGC^GCA
Hin1I 1223 GACGCC G[GA]^CG[CT]C
MamI 3878 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1053 CAATTG C^AATTG
MunI 1053 CAATTG C^AATTG
NaeI 1247 GCCGGC GCC^GGC
NgoMIV 1247 GCCGGC G^CCGGC
NheI 3322 GCTAGC G^CTAGC
NruI 4485 TCGCGA TCG^CGA
NspV 861 TTCGAA TT^CGAA
PmaCI 2190 CACGTG CAC^GTG
PmlI 2190 CACGTG CAC^GTG
PshAI 124 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1561 GAAGAGC GCTCTTC(1/4)
SfuI 861 TTCGAA TT^CGAA
SphI 1816 GCATGC GCATG^C
SspBI 1236 TGTACA T^GTACA
SspI 47 AATATT AAT^ATT
XbaI 1656 TCTAGA T^CTAGA
XcmI 5875 CCAGTGGTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspI 4262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4654 GACCTTGTC
AvrII 2531 CCTAGG C^CTAGG
3214 CCTAGG
BanI 1070 GGTGCC G^G[CT][GA]CC
5554 GGTGCC
BciVI 5533 GGATAC GTATCC(6/5)
3790 GTATCC
BglI 299 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4198 GCCCGTGGGGC
BglII 1105 AGATCT A^GATCT
2638 AGATCT
BlnI 2531 CCTAGG C^CTAGG
3214 CCTAGG
BsaAI 1905 TACGTG [CT]AC^GT[GA]
2190 CACGTG
BsgI 4994 CTGCAC GTGCAG(16/14)
4460 GTGCAG
BsrBI 2120 CCGCTC CCGCTC(-3/-3)
5558 CCGCTC
BssSI 1293 CACGAG CACGAG(-5/-1)
1143 CTCGTG
BstEII 4021 GGTAACC G^GT[ACGT]ACC
5738 GGTTACC
BstPI 4021 GGTAACC G^GT[ACGT]ACC
5738 GGTTACC
DrdI 1752 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3642 GACCCCCCTGTC
EcoO65I 4021 GGTAACC G^GT[ACGT]ACC
5738 GGTTACC
NdeI 3688 CATATG CA^TATG
3819 CATATG
PflFI 4262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4654 GACCTTGTC
SmaI 222 CCCGGG CCC^GGG
5658 CCCGGG
SpeI 787 ACTAGT A^CTAGT
1734 ACTAGT
Tth111I 4262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4654 GACCTTGTC
XmaCI 222 CCCGGG C^CCGGG
5658 CCCGGG
XmaI 222 CCCGGG C^CCGGG
5658 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspDI BspHI BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I ClaI DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII