##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I Aor51HI AscI AseI
AsnI Asp718I AviII BanI BbeI
BbrPI BcgI BclI BglI BsiWI
BspDI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI Eco47III EcoT22I
FbaI FseI FspI HpaI KasI
KpnI KspI MluI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflMI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI Van91I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 2585 TCCGGA T^CCGGA
AclI 2860 AACGTT AA^CGTT
AcyI 3859 GGCGTC G[GA]^CG[CT]C
AgeI 2330 ACCGGT A^CCGGT
AhdI 5813 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5815 CCTAAGG CC^T[ACGT]AGG
ApaI 5252 GGGCCC GGGCC^C
AspEI 5813 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4333 CCTAGG C^CTAGG
BalI 399 TGGCCA TGG^CCA
BcnI 1066 CCCGG CC^[GC]GG
BlnI 4333 CCTAGG C^CTAGG
BlpI 1461 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1461 GCTGAGC GC^T[ACGT]AGC
BsaBI 4712 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3859 GGCGTC G[GA]^CG[CT]C
BseAI 2585 TCCGGA T^CCGGA
BsiEI 154 CGGCCG CG[GA][CT]^CG
BsmI 2752 GAATGC GAATGC(1/-1)
Bsp1407I 5348 TGTACA T^GTACA
BspEI 2585 TCCGGA T^CCGGA
BspHI 2186 TCATGA T^CATGA
BspLU11I 2025 ACATGT A^CATGT
BsrBI 219 CCGCTC CCGCTC(-3/-3)
BsrFI 2330 ACCGGT [GA]^CCGG[CT]
BsrGI 5348 TGTACA T^GTACA
Bsu36I 5815 CCTAAGG CC^T[ACGT]AGG
CelII 1461 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2330 ACCGGT [GA]^CCGG[CT]
DraIII 1140 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5432 GACACCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 154 CGGCCG C^GGCCG
Eam1105I 5813 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 154 CGGCCG C^GGCCG
Eco52I 154 CGGCCG C^GGCCG
Eco81I 5815 CCTAAGG CC^T[ACGT]AGG
EcoRV 5694 GATATC GAT^ATC
HgaI 3860 GCGTC GACGC(5/10)
Hin1I 3859 GGCGTC G[GA]^CG[CT]C
MamI 4712 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3421 CAATTG C^AATTG
MluNI 399 TGGCCA TGG^CCA
MroI 2585 TCCGGA T^CCGGA
MscI 399 TGGCCA TGG^CCA
MunI 3421 CAATTG C^AATTG
NciI 1066 CCCGG CC^[GC]GG
NheI 4634 GCTAGC G^CTAGC
PaeR7I 2603 CTCGAG C^TCGAG
PinAI 2330 ACCGGT A^CCGGT
PpuMI 4590 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 2860 AACGTT AA^CGTT
RcaI 2186 TCATGA T^CATGA
SspBI 5348 TGTACA T^GTACA
SwaI 4267 ATTTAAAT ATTT^AAAT
XbaI 4383 TCTAGA T^CTAGA
XhoI 2603 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 1844 CGCG CG^CG
1883 CGCG
AflII 2233 CTTAAG C^TTAAG
4044 CTTAAG
Alw44I 3698 GTGCAC G^TGCAC
5731 GTGCAC
AlwNI 105 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3442 CAGTTACTG
ApaLI 3698 GTGCAC G^TGCAC
5731 GTGCAC
BamHI 1449 GGATCC G^GATCC
1499 GGATCC
BfrI 2233 CTTAAG C^TTAAG
4044 CTTAAG
BglII 1196 AGATCT A^GATCT
2946 AGATCT
BsaAI 3157 TACGTG [CT]AC^GT[GA]
3365 CACGTA
BsaI 1272 GAGACC GGTCTC(1/5)
3755 GGTCTC
BsaWI 2330 ACCGGT [AT]^CCGG[AT]
2585 TCCGGA
BstEII 1749 GGTCACC G^GT[ACGT]ACC
4561 GGTCACC
BstPI 1749 GGTCACC G^GT[ACGT]ACC
4561 GGTCACC
BstUI 1844 CGCG CG^CG
1883 CGCG
BstXI 1039 CCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1972 CCAAAGCACTGG
EcoO65I 1749 GGTCACC G^GT[ACGT]ACC
4561 GGTCACC
MvnI 1844 CGCG CG^CG
1883 CGCG
SacI 1894 GAGCTC GAGCT^C
3232 GAGCTC
ScaI 4056 AGTACT AGT^ACT
4259 AGTACT
SexAI 1745 ACCAGGT A^CC[AT]GGT
4847 ACCTGGT
SphI 1730 GCATGC GCATG^C
2789 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AspI AvaI AvaII BanII BbsI
BbvCI BbvI BciVI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BssSI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII XmnI