##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI AocI AscI AseI AsnI
Asp718I AspEI AviII BamHI BbeI
BbvCI BcgI BclI BglII BlpI
Bpu1102I BsaHI BsiWI BsmBI BspDI
BspHI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI Eam1105I Eco81I EcoRV EcoT22I
FbaI FspI Hin1I HindIII KasI
KpnI MfeI MluI MunI NarI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SfoI SfuI
SgrAI SphI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 953 TCCGGA T^CCGGA
AflII 2592 CTTAAG C^TTAAG
Alw44I 1031 GTGCAC G^TGCAC
ApaLI 1031 GTGCAC G^TGCAC
Asp700I 265 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1790 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3087 CACGTG CAC^GTG
BbsI 539 GAAGAC GAAGAC(2/6)
BciVI 4519 GGATAC GTATCC(6/5)
BfrI 2592 CTTAAG C^TTAAG
BpuAI 539 GAAGAC GAAGAC(2/6)
BsaBI 652 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 953 TCCGGA T^CCGGA
BsgI 1164 GTGCAG GTGCAG(16/14)
BspEI 953 TCCGGA T^CCGGA
BspLU11I 4333 ACATGT A^CATGT
BsrFI 1343 GCCGGC [GA]^CCGG[CT]
BstAPI 3310 GCACCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1343 GCCGGC [GA]^CCGG[CT]
DraI 1881 TTTAAA TTT^AAA
DraIII 2079 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3029 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1342 GGCCGGCC GGCCGG^CC
HpaI 3262 GTTAAC GTT^AAC
KspI 213 CCGCGG CCGC^GG
MamI 652 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 953 TCCGGA T^CCGGA
NaeI 1343 GCCGGC GCC^GGC
NgoMIV 1343 GCCGGC G^CCGGC
PflFI 1790 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3087 CACGTG CAC^GTG
PmlI 3087 CACGTG CAC^GTG
SacI 152 GAGCTC GAGCT^C
SacII 213 CCGCGG CCGC^GG
ScaI 441 AGTACT AGT^ACT
SfiI 3645 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3191 CCCGGG CCC^GGG
SnaBI 3221 TACGTA TAC^GTA
SpeI 2443 ACTAGT A^CTAGT
Tth111I 1790 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3191 CCCGGG C^CCGGG
XmaI 3191 CCCGGG C^CCGGG
XmnI 265 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Aor51HI 279 AGCGCT AGC^GCT
3827 AGCGCT
AvrII 2987 CCTAGG C^CTAGG
3332 CCTAGG
BalI 839 TGGCCA TGG^CCA
1771 TGGCCA
BlnI 2987 CCTAGG C^CTAGG
3332 CCTAGG
BsiEI 146 CGGCCG CG[GA][CT]^CG
216 CGGCCG
Bsp1407I 3609 TGTACA T^GTACA
4038 TGTACA
BsrGI 3609 TGTACA T^GTACA
4038 TGTACA
BstXI 2696 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3823 CCAGAGCGCTGG
BstYI 2831 GGATCT [GA]^GATC[CT]
3803 AGATCC
DsaI 213 CCGCGG C^C[GA][CT]GG
303 CCGTGG
EagI 146 CGGCCG C^GGCCG
216 CGGCCG
EclXI 146 CGGCCG C^GGCCG
216 CGGCCG
Eco47III 279 AGCGCT AGC^GCT
3827 AGCGCT
Eco52I 146 CGGCCG C^GGCCG
216 CGGCCG
HgaI 2872 GACGC GACGC(5/10)
57 GCGTC
MflI 2831 GGATCT [GA]^GATC[CT]
3803 AGATCC
MluNI 839 TGGCCA TGG^CCA
1771 TGGCCA
MscI 839 TGGCCA TGG^CCA
1771 TGGCCA
PflMI 675 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3691 CCAGGTGCTGG
PshAI 1062 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3491 GACCCCAGTC
SexAI 797 ACCTGGT A^CC[AT]GGT
898 ACCAGGT
SspBI 3609 TGTACA T^GTACA
4038 TGTACA
StuI 290 AGGCCT AGG^CCT
2746 AGGCCT
Van91I 675 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3691 CCAGGTGCTGG
XbaI 2645 TCTAGA T^CTAGA
3229 TCTAGA
XhoII 2831 GGATCT [GA]^GATC[CT]
3803 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoO65I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NheI NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI