################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AviII BamHI BbeI BbvCI BcgI BclI BglII BlpI Bpu1102I BsaHI BsiWI BsmBI BspDI BspHI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DrdI Eam1105I Eco81I EcoRV EcoT22I FbaI FspI Hin1I HindIII KasI KpnI MfeI MluI MunI NarI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfoI SfuI SgrAI SphI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 953 TCCGGA T^CCGGA AflII 2592 CTTAAG C^TTAAG Alw44I 1031 GTGCAC G^TGCAC ApaLI 1031 GTGCAC G^TGCAC Asp700I 265 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1790 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 3087 CACGTG CAC^GTG BbsI 539 GAAGAC GAAGAC(2/6) BciVI 4519 GGATAC GTATCC(6/5) BfrI 2592 CTTAAG C^TTAAG BpuAI 539 GAAGAC GAAGAC(2/6) BsaBI 652 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 953 TCCGGA T^CCGGA BsgI 1164 GTGCAG GTGCAG(16/14) BspEI 953 TCCGGA T^CCGGA BspLU11I 4333 ACATGT A^CATGT BsrFI 1343 GCCGGC [GA]^CCGG[CT] BstAPI 3310 GCACCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 1343 GCCGGC [GA]^CCGG[CT] DraI 1881 TTTAAA TTT^AAA DraIII 2079 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 3029 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 1342 GGCCGGCC GGCCGG^CC HpaI 3262 GTTAAC GTT^AAC KspI 213 CCGCGG CCGC^GG MamI 652 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 953 TCCGGA T^CCGGA NaeI 1343 GCCGGC GCC^GGC NgoMIV 1343 GCCGGC G^CCGGC PflFI 1790 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3087 CACGTG CAC^GTG PmlI 3087 CACGTG CAC^GTG SacI 152 GAGCTC GAGCT^C SacII 213 CCGCGG CCGC^GG ScaI 441 AGTACT AGT^ACT SfiI 3645 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 3191 CCCGGG CCC^GGG SnaBI 3221 TACGTA TAC^GTA SpeI 2443 ACTAGT A^CTAGT Tth111I 1790 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 3191 CCCGGG C^CCGGG XmaI 3191 CCCGGG C^CCGGG XmnI 265 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Aor51HI 279 AGCGCT AGC^GCT 3827 AGCGCT AvrII 2987 CCTAGG C^CTAGG 3332 CCTAGG BalI 839 TGGCCA TGG^CCA 1771 TGGCCA BlnI 2987 CCTAGG C^CTAGG 3332 CCTAGG BsiEI 146 CGGCCG CG[GA][CT]^CG 216 CGGCCG Bsp1407I 3609 TGTACA T^GTACA 4038 TGTACA BsrGI 3609 TGTACA T^GTACA 4038 TGTACA BstXI 2696 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3823 CCAGAGCGCTGG BstYI 2831 GGATCT [GA]^GATC[CT] 3803 AGATCC DsaI 213 CCGCGG C^C[GA][CT]GG 303 CCGTGG EagI 146 CGGCCG C^GGCCG 216 CGGCCG EclXI 146 CGGCCG C^GGCCG 216 CGGCCG Eco47III 279 AGCGCT AGC^GCT 3827 AGCGCT Eco52I 146 CGGCCG C^GGCCG 216 CGGCCG HgaI 2872 GACGC GACGC(5/10) 57 GCGTC MflI 2831 GGATCT [GA]^GATC[CT] 3803 AGATCC MluNI 839 TGGCCA TGG^CCA 1771 TGGCCA MscI 839 TGGCCA TGG^CCA 1771 TGGCCA PflMI 675 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3691 CCAGGTGCTGG PshAI 1062 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3491 GACCCCAGTC SexAI 797 ACCTGGT A^CC[AT]GGT 898 ACCAGGT SspBI 3609 TGTACA T^GTACA 4038 TGTACA StuI 290 AGGCCT AGG^CCT 2746 AGGCCT Van91I 675 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3691 CCAGGTGCTGG XbaI 2645 TCTAGA T^CTAGA 3229 TCTAGA XhoII 2831 GGATCT [GA]^GATC[CT] 3803 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NheI NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI