################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccII AflII AgeI ApaI AscI AviII AvrII BbeI BbvCI BcgI BfrI BglI BlnI BsaBI BsgI BsiEI BsiWI Bsp1407I BspDI BsrBI BsrGI BssHII BssSI BstBI BstUI ClaI CpoI EagI EclXI Eco52I EcoT22I FseI FspI KasI KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SpeI Sse8387I SspBI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1597 GGTACC G^GTACC AclI 744 AACGTT AA^CGTT AcyI 5148 GGCGTC G[GA]^CG[CT]C AhdI 1888 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2128 CCTCAGG CC^T[ACGT]AGG Aor51HI 847 AGCGCT AGC^GCT Asp718I 1597 GGTACC G^GTACC AspEI 1888 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1084 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 553 CCCGGG C^[CT]CG[GA]G BamHI 129 GGATCC G^GATCC BanI 1597 GGTACC G^G[CT][GA]CC BbrPI 4457 CACGTG CAC^GTG BciVI 5451 GTATCC GTATCC(6/5) BclI 269 TGATCA T^GATCA BlpI 1693 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1693 GCTCAGC GC^T[ACGT]AGC BsaHI 5148 GGCGTC G[GA]^CG[CT]C BsmBI 1355 CGTCTC CGTCTC(1/5) BsoBI 553 CCCGGG C^[CT]CG[GA]G BspMI 888 GCAGGT ACCTGC(4/8) BsrFI 3088 ACCGGC [GA]^CCGG[CT] BstEII 2594 GGTCACC G^GT[ACGT]ACC BstPI 2594 GGTCACC G^GT[ACGT]ACC Bsu36I 2128 CCTCAGG CC^T[ACGT]AGG CelII 1693 GCTCAGC GC^T[ACGT]AGC Cfr10I 3088 ACCGGC [GA]^CCGG[CT] DrdI 3998 GACCTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1888 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 847 AGCGCT AGC^GCT Eco81I 2128 CCTCAGG CC^T[ACGT]AGG EcoNI 1737 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2594 GGTCACC G^GT[ACGT]ACC EcoRI 3121 GAATTC G^AATTC FbaI 269 TGATCA T^GATCA Hin1I 5148 GGCGTC G[GA]^CG[CT]C HindIII 4579 AAGCTT A^AGCTT KpnI 1597 GGTACC GGTAC^C NcoI 2330 CCATGG C^CATGG PflFI 1084 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4457 CACGTG CAC^GTG PmlI 4457 CACGTG CAC^GTG PshAI 1506 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 744 AACGTT AA^CGTT SmaI 553 CCCGGG CCC^GGG SnaBI 117 TACGTA TAC^GTA SphI 2216 GCATGC GCATG^C TaqI 5254 TCGA T^CGA Tth111I 1084 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 5254 TCGA T^CGA XmaCI 553 CCCGGG C^CCGGG XmaI 553 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 4144 GTATAC GT^[AC][GT]AC 4997 GTATAC AccIII 126 TCCGGA T^CCGGA 2887 TCCGGA Alw44I 425 GTGCAC G^TGCAC 1307 GTGCAC ApaLI 425 GTGCAC G^TGCAC 1307 GTGCAC AseI 3935 ATTAAT AT^TAAT 5436 ATTAAT AsnI 3935 ATTAAT AT^TAAT 5436 ATTAAT Asp700I 1019 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2735 GAAGCACTTC BanII 1869 GGGCTC G[GA]GC[CT]^C 4288 GGGCTC BbsI 3850 GTCTTC GAAGAC(2/6) 4110 GTCTTC BcnI 553 CCCGG CC^[GC]GG 554 CCGGG BpuAI 3850 GTCTTC GAAGAC(2/6) 4110 GTCTTC BsaAI 117 TACGTA [CT]AC^GT[GA] 4457 CACGTG BsaI 514 GAGACC GGTCTC(1/5) 4879 GAGACC BseAI 126 TCCGGA T^CCGGA 2887 TCCGGA BsmFI 556 GGGAC GGGAC(10/14) 4719 GGGAC BsmI 2325 GAATGC GAATGC(1/-1) 2855 GCATTC BspEI 126 TCCGGA T^CCGGA 2887 TCCGGA BspHI 33 TCATGA T^CATGA 99 TCATGA BspLU11I 4223 ACATGT A^CATGT 4658 ACATGT Bst1107I 4144 GTATAC GTA^TAC 4997 GTATAC BstAPI 568 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2472 GCAGCCCATGC BstXI 3993 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4278 CCACAGCCTTGG BstZ17I 4144 GTATAC GTA^TAC 4997 GTATAC DraI 4313 TTTAAA TTT^AAA 4584 TTTAAA DraII 556 GGGACCT [GA]G^G[ACGT]CC[CT] 2523 AGGTCCT DraIII 5140 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG 5306 CACTCAGTG DsaI 2330 CCATGG C^C[GA][CT]GG 4418 CCGTGG EcoO109I 556 GGGACCT [GA]G^G[ACGT]CC[CT] 2523 AGGTCCT EcoRV 168 GATATC GAT^ATC 3144 GATATC HaeII 847 AGCGCT [GA]GCGC^[CT] 2201 AGCGCC MroI 126 TCCGGA T^CCGGA 2887 TCCGGA NciI 553 CCCGG CC^[GC]GG 554 CCGGG PpuMI 556 GGGACCT [GA]G^G[AT]CC[CT] 2523 AGGTCCT PshBI 3935 ATTAAT AT^TAAT 5436 ATTAAT RcaI 33 TCATGA T^CATGA 99 TCATGA SapI 1960 GAAGAGC GCTCTTC(1/4) 1871 GCTCTTC ScaI 808 AGTACT AGT^ACT 3833 AGTACT XcmI 1397 CCACGCCACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2330 CCATGGCTACAATGG XmnI 1019 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2735 GAAGCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BbvI BfaI BglII BmyI BpmI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII