##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccII AflII AgeI ApaI
AscI AviII AvrII BbeI BbvCI
BcgI BfrI BglI BlnI BsaBI
BsgI BsiEI BsiWI Bsp1407I BspDI
BsrBI BsrGI BssHII BssSI BstBI
BstUI ClaI CpoI EagI EclXI
Eco52I EcoT22I FseI FspI KasI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SpeI Sse8387I SspBI StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1597 GGTACC G^GTACC
AclI 744 AACGTT AA^CGTT
AcyI 5148 GGCGTC G[GA]^CG[CT]C
AhdI 1888 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2128 CCTCAGG CC^T[ACGT]AGG
Aor51HI 847 AGCGCT AGC^GCT
Asp718I 1597 GGTACC G^GTACC
AspEI 1888 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1084 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 553 CCCGGG C^[CT]CG[GA]G
BamHI 129 GGATCC G^GATCC
BanI 1597 GGTACC G^G[CT][GA]CC
BbrPI 4457 CACGTG CAC^GTG
BciVI 5451 GTATCC GTATCC(6/5)
BclI 269 TGATCA T^GATCA
BlpI 1693 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1693 GCTCAGC GC^T[ACGT]AGC
BsaHI 5148 GGCGTC G[GA]^CG[CT]C
BsmBI 1355 CGTCTC CGTCTC(1/5)
BsoBI 553 CCCGGG C^[CT]CG[GA]G
BspMI 888 GCAGGT ACCTGC(4/8)
BsrFI 3088 ACCGGC [GA]^CCGG[CT]
BstEII 2594 GGTCACC G^GT[ACGT]ACC
BstPI 2594 GGTCACC G^GT[ACGT]ACC
Bsu36I 2128 CCTCAGG CC^T[ACGT]AGG
CelII 1693 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3088 ACCGGC [GA]^CCGG[CT]
DrdI 3998 GACCTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1888 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 847 AGCGCT AGC^GCT
Eco81I 2128 CCTCAGG CC^T[ACGT]AGG
EcoNI 1737 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2594 GGTCACC G^GT[ACGT]ACC
EcoRI 3121 GAATTC G^AATTC
FbaI 269 TGATCA T^GATCA
Hin1I 5148 GGCGTC G[GA]^CG[CT]C
HindIII 4579 AAGCTT A^AGCTT
KpnI 1597 GGTACC GGTAC^C
NcoI 2330 CCATGG C^CATGG
PflFI 1084 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4457 CACGTG CAC^GTG
PmlI 4457 CACGTG CAC^GTG
PshAI 1506 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 744 AACGTT AA^CGTT
SmaI 553 CCCGGG CCC^GGG
SnaBI 117 TACGTA TAC^GTA
SphI 2216 GCATGC GCATG^C
TaqI 5254 TCGA T^CGA
Tth111I 1084 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 5254 TCGA T^CGA
XmaCI 553 CCCGGG C^CCGGG
XmaI 553 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 4144 GTATAC GT^[AC][GT]AC
4997 GTATAC
AccIII 126 TCCGGA T^CCGGA
2887 TCCGGA
Alw44I 425 GTGCAC G^TGCAC
1307 GTGCAC
ApaLI 425 GTGCAC G^TGCAC
1307 GTGCAC
AseI 3935 ATTAAT AT^TAAT
5436 ATTAAT
AsnI 3935 ATTAAT AT^TAAT
5436 ATTAAT
Asp700I 1019 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2735 GAAGCACTTC
BanII 1869 GGGCTC G[GA]GC[CT]^C
4288 GGGCTC
BbsI 3850 GTCTTC GAAGAC(2/6)
4110 GTCTTC
BcnI 553 CCCGG CC^[GC]GG
554 CCGGG
BpuAI 3850 GTCTTC GAAGAC(2/6)
4110 GTCTTC
BsaAI 117 TACGTA [CT]AC^GT[GA]
4457 CACGTG
BsaI 514 GAGACC GGTCTC(1/5)
4879 GAGACC
BseAI 126 TCCGGA T^CCGGA
2887 TCCGGA
BsmFI 556 GGGAC GGGAC(10/14)
4719 GGGAC
BsmI 2325 GAATGC GAATGC(1/-1)
2855 GCATTC
BspEI 126 TCCGGA T^CCGGA
2887 TCCGGA
BspHI 33 TCATGA T^CATGA
99 TCATGA
BspLU11I 4223 ACATGT A^CATGT
4658 ACATGT
Bst1107I 4144 GTATAC GTA^TAC
4997 GTATAC
BstAPI 568 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2472 GCAGCCCATGC
BstXI 3993 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4278 CCACAGCCTTGG
BstZ17I 4144 GTATAC GTA^TAC
4997 GTATAC
DraI 4313 TTTAAA TTT^AAA
4584 TTTAAA
DraII 556 GGGACCT [GA]G^G[ACGT]CC[CT]
2523 AGGTCCT
DraIII 5140 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
5306 CACTCAGTG
DsaI 2330 CCATGG C^C[GA][CT]GG
4418 CCGTGG
EcoO109I 556 GGGACCT [GA]G^G[ACGT]CC[CT]
2523 AGGTCCT
EcoRV 168 GATATC GAT^ATC
3144 GATATC
HaeII 847 AGCGCT [GA]GCGC^[CT]
2201 AGCGCC
MroI 126 TCCGGA T^CCGGA
2887 TCCGGA
NciI 553 CCCGG CC^[GC]GG
554 CCGGG
PpuMI 556 GGGACCT [GA]G^G[AT]CC[CT]
2523 AGGTCCT
PshBI 3935 ATTAAT AT^TAAT
5436 ATTAAT
RcaI 33 TCATGA T^CATGA
99 TCATGA
SapI 1960 GAAGAGC GCTCTTC(1/4)
1871 GCTCTTC
ScaI 808 AGTACT AGT^ACT
3833 AGTACT
XcmI 1397 CCACGCCACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2330 CCATGGCTACAATGG
XmnI 1019 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2735 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BbvI BfaI BglII BmyI
BpmI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII