##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AflII AgeI Aor51HI
ApaI AscI AviII AvrII BbeI
BbsI BclI BfrI BlnI BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BssHII BstBI BstXI
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoRV FbaI
FseI FspI HaeII HpaI KasI
KspI MamI MluI MroI NarI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PvuI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3613 GGTACC G^GTACC
AhdI 3592 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1369 CCTTAGG CC^T[ACGT]AGG
AseI 2899 ATTAAT AT^TAAT
AsnI 2899 ATTAAT AT^TAAT
Asp718I 3613 GGTACC G^GTACC
AspEI 3592 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2357 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5006 TGGCCA TGG^CCA
BbrPI 4946 CACGTG CAC^GTG
BbvCI 1221 CCTCAGC CCTCAGC(-5/-2)
BcgI 809 CGACCTACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5118 GGATAC GTATCC(6/5)
BglII 2510 AGATCT A^GATCT
BsaAI 4946 CACGTG [CT]AC^GT[GA]
BsmI 3822 GCATTC GAATGC(1/-1)
Bsp1407I 2764 TGTACA T^GTACA
BspHI 3045 TCATGA T^CATGA
BsrBI 4889 CCGCTC CCGCTC(-3/-3)
BsrGI 2764 TGTACA T^GTACA
Bst1107I 483 GTATAC GTA^TAC
BstAPI 2681 GCACACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1103 GGTGACC G^GT[ACGT]ACC
BstPI 1103 GGTGACC G^GT[ACGT]ACC
BstZ17I 483 GTATAC GTA^TAC
Bsu36I 1369 CCTTAGG CC^T[ACGT]AGG
Eam1105I 3592 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1369 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1103 GGTGACC G^GT[ACGT]ACC
EcoRI 3034 GAATTC G^AATTC
FauI 147 GCGGG CCCGC(4/6)
HindIII 4294 AAGCTT A^AGCTT
KpnI 3613 GGTACC GGTAC^C
MfeI 3924 CAATTG C^AATTG
MluNI 5006 TGGCCA TGG^CCA
MscI 5006 TGGCCA TGG^CCA
MunI 3924 CAATTG C^AATTG
NdeI 4196 CATATG CA^TATG
PflFI 2357 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4946 CACGTG CAC^GTG
PmlI 4946 CACGTG CAC^GTG
PshBI 2899 ATTAAT AT^TAAT
RcaI 3045 TCATGA T^CATGA
SacI 2662 GAGCTC GAGCT^C
SapI 2221 GCTCTTC GCTCTTC(1/4)
SbfI 2439 CCTGCAGG CCTGCA^GG
ScaI 2034 AGTACT AGT^ACT
SphI 2024 GCATGC GCATG^C
Sse8387I 2439 CCTGCAGG CCTGCA^GG
SspBI 2764 TGTACA T^GTACA
StuI 221 AGGCCT AGG^CCT
Tth111I 2357 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 483 GTATAC GT^[AC][GT]AC
571 GTCTAC
AccII 668 CGCG CG^CG
1840 CGCG
AclI 3062 AACGTT AA^CGTT
4808 AACGTT
AcyI 2741 GACGCC G[GA]^CG[CT]C
4919 GACGCC
AlwNI 1762 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2181 CAGACTCTG
AvaI 63 CCCGGG C^[CT]CG[GA]G
3990 CCCGGG
BamHI 2189 GGATCC G^GATCC
2606 GGATCC
BlpI 1923 GCTAAGC GC^T[ACGT]AGC
3746 GCTTAGC
Bpu1102I 1923 GCTAAGC GC^T[ACGT]AGC
3746 GCTTAGC
BsaHI 2741 GACGCC G[GA]^CG[CT]C
4919 GACGCC
BsmBI 190 CGTCTC CGTCTC(1/5)
778 CGTCTC
BsoBI 63 CCCGGG C^[CT]CG[GA]G
3990 CCCGGG
BspMI 4191 ACCTGC ACCTGC(4/8)
4235 GCAGGT
BsrFI 1087 GCCGGC [GA]^CCGG[CT]
2337 GCCGGC
BstUI 668 CGCG CG^CG
1840 CGCG
CelII 1923 GCTAAGC GC^T[ACGT]AGC
3746 GCTTAGC
CfoI 1629 GCGC GCG^C
1841 GCGC
Cfr10I 1087 GCCGGC [GA]^CCGG[CT]
2337 GCCGGC
EaeI 3188 CGGCCA [CT]^GGCC[GA]
5006 TGGCCA
HhaI 1629 GCGC GCG^C
1841 GCGC
Hin1I 2741 GACGCC G[GA]^CG[CT]C
4919 GACGCC
HinP1I 1629 GCGC G^CGC
1841 GCGC
HincII 408 GTTGAC GT[CT]^[GA]AC
1073 GTCAAC
HindII 408 GTTGAC GT[CT]^[GA]AC
1073 GTCAAC
MvnI 668 CGCG CG^CG
1840 CGCG
NaeI 1087 GCCGGC GCC^GGC
2337 GCCGGC
NcoI 1290 CCATGG C^CATGG
1559 CCATGG
NgoMIV 1087 GCCGGC G^CCGGC
2337 GCCGGC
PpuMI 1763 AGGTCCT [GA]G^G[AT]CC[CT]
3181 AGGACCT
PshAI 1816 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4919 GACGCCTGTC
Psp1406I 3062 AACGTT AA^CGTT
4808 AACGTT
SexAI 1931 ACCAGGT A^CC[AT]GGT
4983 ACCAGGT
SmaI 63 CCCGGG CCC^GGG
3990 CCCGGG
XmaCI 63 CCCGGG C^CCGGG
3990 CCCGGG
XmaI 63 CCCGGG C^CCGGG
3990 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspHI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrDI BsrI BssKI BssSI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI