################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccIII AflII AgeI Aor51HI ApaI AscI AviII AvrII BbeI BbsI BclI BfrI BlnI BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BssHII BstBI BstXI ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoRV FbaI FseI FspI HaeII HpaI KasI KspI MamI MluI MroI NarI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 3613 GGTACC G^GTACC AhdI 3592 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1369 CCTTAGG CC^T[ACGT]AGG AseI 2899 ATTAAT AT^TAAT AsnI 2899 ATTAAT AT^TAAT Asp718I 3613 GGTACC G^GTACC AspEI 3592 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2357 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 5006 TGGCCA TGG^CCA BbrPI 4946 CACGTG CAC^GTG BbvCI 1221 CCTCAGC CCTCAGC(-5/-2) BcgI 809 CGACCTACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5118 GGATAC GTATCC(6/5) BglII 2510 AGATCT A^GATCT BsaAI 4946 CACGTG [CT]AC^GT[GA] BsmI 3822 GCATTC GAATGC(1/-1) Bsp1407I 2764 TGTACA T^GTACA BspHI 3045 TCATGA T^CATGA BsrBI 4889 CCGCTC CCGCTC(-3/-3) BsrGI 2764 TGTACA T^GTACA Bst1107I 483 GTATAC GTA^TAC BstAPI 2681 GCACACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1103 GGTGACC G^GT[ACGT]ACC BstPI 1103 GGTGACC G^GT[ACGT]ACC BstZ17I 483 GTATAC GTA^TAC Bsu36I 1369 CCTTAGG CC^T[ACGT]AGG Eam1105I 3592 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1369 CCTTAGG CC^T[ACGT]AGG EcoO65I 1103 GGTGACC G^GT[ACGT]ACC EcoRI 3034 GAATTC G^AATTC FauI 147 GCGGG CCCGC(4/6) HindIII 4294 AAGCTT A^AGCTT KpnI 3613 GGTACC GGTAC^C MfeI 3924 CAATTG C^AATTG MluNI 5006 TGGCCA TGG^CCA MscI 5006 TGGCCA TGG^CCA MunI 3924 CAATTG C^AATTG NdeI 4196 CATATG CA^TATG PflFI 2357 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4946 CACGTG CAC^GTG PmlI 4946 CACGTG CAC^GTG PshBI 2899 ATTAAT AT^TAAT RcaI 3045 TCATGA T^CATGA SacI 2662 GAGCTC GAGCT^C SapI 2221 GCTCTTC GCTCTTC(1/4) SbfI 2439 CCTGCAGG CCTGCA^GG ScaI 2034 AGTACT AGT^ACT SphI 2024 GCATGC GCATG^C Sse8387I 2439 CCTGCAGG CCTGCA^GG SspBI 2764 TGTACA T^GTACA StuI 221 AGGCCT AGG^CCT Tth111I 2357 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 483 GTATAC GT^[AC][GT]AC 571 GTCTAC AccII 668 CGCG CG^CG 1840 CGCG AclI 3062 AACGTT AA^CGTT 4808 AACGTT AcyI 2741 GACGCC G[GA]^CG[CT]C 4919 GACGCC AlwNI 1762 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG 2181 CAGACTCTG AvaI 63 CCCGGG C^[CT]CG[GA]G 3990 CCCGGG BamHI 2189 GGATCC G^GATCC 2606 GGATCC BlpI 1923 GCTAAGC GC^T[ACGT]AGC 3746 GCTTAGC Bpu1102I 1923 GCTAAGC GC^T[ACGT]AGC 3746 GCTTAGC BsaHI 2741 GACGCC G[GA]^CG[CT]C 4919 GACGCC BsmBI 190 CGTCTC CGTCTC(1/5) 778 CGTCTC BsoBI 63 CCCGGG C^[CT]CG[GA]G 3990 CCCGGG BspMI 4191 ACCTGC ACCTGC(4/8) 4235 GCAGGT BsrFI 1087 GCCGGC [GA]^CCGG[CT] 2337 GCCGGC BstUI 668 CGCG CG^CG 1840 CGCG CelII 1923 GCTAAGC GC^T[ACGT]AGC 3746 GCTTAGC CfoI 1629 GCGC GCG^C 1841 GCGC Cfr10I 1087 GCCGGC [GA]^CCGG[CT] 2337 GCCGGC EaeI 3188 CGGCCA [CT]^GGCC[GA] 5006 TGGCCA HhaI 1629 GCGC GCG^C 1841 GCGC Hin1I 2741 GACGCC G[GA]^CG[CT]C 4919 GACGCC HinP1I 1629 GCGC G^CGC 1841 GCGC HincII 408 GTTGAC GT[CT]^[GA]AC 1073 GTCAAC HindII 408 GTTGAC GT[CT]^[GA]AC 1073 GTCAAC MvnI 668 CGCG CG^CG 1840 CGCG NaeI 1087 GCCGGC GCC^GGC 2337 GCCGGC NcoI 1290 CCATGG C^CATGG 1559 CCATGG NgoMIV 1087 GCCGGC G^CCGGC 2337 GCCGGC PpuMI 1763 AGGTCCT [GA]G^G[AT]CC[CT] 3181 AGGACCT PshAI 1816 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4919 GACGCCTGTC Psp1406I 3062 AACGTT AA^CGTT 4808 AACGTT SexAI 1931 ACCAGGT A^CC[AT]GGT 4983 ACCAGGT SmaI 63 CCCGGG CCC^GGG 3990 CCCGGG XmaCI 63 CCCGGG C^CCGGG 3990 CCCGGG XmaI 63 CCCGGG C^CCGGG 3990 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI ApaLI ApoI Asp700I AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrDI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI