##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI Alw44I ApaLI AscI
AvrII BbeI BbvCI BciVI BclI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmI BspDI BspHI BstBI CelII
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV FbaI FseI HpaI
KasI MamI MfeI MluI MunI
NarI NotI NruI NspV PacI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 4863 GGTACC G^GTACC
AccIII 4705 TCCGGA T^CCGGA
AcyI 2414 GGCGTC G[GA]^CG[CT]C
AflII 3305 CTTAAG C^TTAAG
AgeI 35 ACCGGT A^CCGGT
AhdI 226 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2419 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1739 AGCGCT AGC^GCT
Asp700I 449 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4863 GGTACC G^GTACC
AspEI 226 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1556 TGCGCA TGC^GCA
BfrI 3305 CTTAAG C^TTAAG
BglII 1383 AGATCT A^GATCT
BsaHI 2414 GGCGTC G[GA]^CG[CT]C
BseAI 4705 TCCGGA T^CCGGA
BsiEI 1671 CGACCG CG[GA][CT]^CG
BsmBI 3178 CGTCTC CGTCTC(1/5)
Bsp1407I 3878 TGTACA T^GTACA
BspEI 4705 TCCGGA T^CCGGA
BspMI 407 GCAGGT ACCTGC(4/8)
BsrBI 1536 CCGCTC CCGCTC(-3/-3)
BsrGI 3878 TGTACA T^GTACA
Bst1107I 2319 GTATAC GTA^TAC
BstZ17I 2319 GTATAC GTA^TAC
Bsu36I 2419 CCTGAGG CC^T[ACGT]AGG
Eam1105I 226 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1739 AGCGCT AGC^GCT
Eco81I 2419 CCTGAGG CC^T[ACGT]AGG
EcoT22I 4570 ATGCAT ATGCA^T
FspI 1556 TGCGCA TGC^GCA
Hin1I 2414 GGCGTC G[GA]^CG[CT]C
HincII 480 GTCAAC GT[CT]^[GA]AC
HindII 480 GTCAAC GT[CT]^[GA]AC
KpnI 4863 GGTACC GGTAC^C
KspI 165 CCGCGG CCGC^GG
MroI 4705 TCCGGA T^CCGGA
NaeI 147 GCCGGC GCC^GGC
NdeI 1405 CATATG CA^TATG
NgoMIV 147 GCCGGC G^CCGGC
NsiI 4570 ATGCAT ATGCA^T
PaeR7I 812 CTCGAG C^TCGAG
PinAI 35 ACCGGT A^CCGGT
SacII 165 CCGCGG CCGC^GG
SmaI 4737 CCCGGG CCC^GGG
SphI 2147 GCATGC GCATG^C
SspBI 3878 TGTACA T^GTACA
XcmI 391 CCAGGCGTAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 812 CTCGAG C^TCGAG
XmaCI 4737 CCCGGG C^CCGGG
XmaI 4737 CCCGGG C^CCGGG
XmnI 449 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2176 GTCTAC GT^[AC][GT]AC
2319 GTATAC
AlwNI 240 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
2565 CAGAAGCTG
ApaI 2573 GGGCCC GGGCC^C
4412 GGGCCC
AseI 874 ATTAAT AT^TAAT
4475 ATTAAT
AsnI 874 ATTAAT AT^TAAT
4475 ATTAAT
AspI 2582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4709 GACCCAGTC
BalI 4127 TGGCCA TGG^CCA
4699 TGGCCA
BbrPI 1585 CACGTG CAC^GTG
2984 CACGTG
BglI 929 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2205 GCCCGTGAGGC
BsaI 1775 GAGACC GGTCTC(1/5)
852 GGTCTC
BsaWI 35 ACCGGT [AT]^CCGG[AT]
4705 TCCGGA
BspLU11I 3611 ACATGT A^CATGT
4583 ACATGT
BstAPI 872 GCATTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3000 GCAGGCCTTGC
BstEII 410 GGTCACC G^GT[ACGT]ACC
4743 GGTCACC
BstPI 410 GGTCACC G^GT[ACGT]ACC
4743 GGTCACC
BstXI 1220 CCAGCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5001 CCACACAACTGG
EcoNI 401 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3483 CCTGTTGGAGG
EcoO65I 410 GGTCACC G^GT[ACGT]ACC
4743 GGTCACC
EcoRI 216 GAATTC G^AATTC
2473 GAATTC
HindIII 2711 AAGCTT A^AGCTT
3793 AAGCTT
MluNI 4127 TGGCCA TGG^CCA
4699 TGGCCA
MscI 4127 TGGCCA TGG^CCA
4699 TGGCCA
NcoI 562 CCATGG C^CATGG
825 CCATGG
NheI 182 GCTAGC G^CTAGC
3664 GCTAGC
PflFI 2582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4709 GACCCAGTC
PflMI 2632 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2963 CCATGTTCTGG
PmaCI 1585 CACGTG CAC^GTG
2984 CACGTG
PmlI 1585 CACGTG CAC^GTG
2984 CACGTG
PpuMI 15 GGGACCC [GA]G^G[AT]CC[CT]
2436 GGGACCC
PshBI 874 ATTAAT AT^TAAT
4475 ATTAAT
SexAI 2689 ACCTGGT A^CC[AT]GGT
4859 ACCTGGT
SspI 452 AATATT AAT^ATT
4636 AATATT
StuI 3002 AGGCCT AGG^CCT
3658 AGGCCT
Tth111I 2582 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4709 GACCCAGTC
Van91I 2632 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2963 CCATGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvI BcgI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII