##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AspEI BclI BglI BsaBI
BsiEI BsiWI BsrFI BssHII BssSI
Bst1107I BstZ17I Cfr10I CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FbaI FseI KspI MamI MluI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmeI
Psp1406I PvuI RsrII SacII SapI
ScaI SfiI SgrAI SmaI SnaBI
StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflII 5179 CTTAAG C^TTAAG
AseI 5236 ATTAAT AT^TAAT
AsnI 5236 ATTAAT AT^TAAT
AspI 3868 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2911 GGATCC G^GATCC
BciVI 5775 GTATCC GTATCC(6/5)
BfrI 5179 CTTAAG C^TTAAG
BsmBI 1240 CGTCTC CGTCTC(1/5)
BspDI 2216 ATCGAT AT^CGAT
BspLU11I 161 ACATGT A^CATGT
BsrBI 1940 GAGCGG CCGCTC(-3/-3)
BstBI 738 TTCGAA TT^CGAA
BstEII 1498 GGTCACC G^GT[ACGT]ACC
BstPI 1498 GGTCACC G^GT[ACGT]ACC
ClaI 2216 ATCGAT AT^CGAT
DrdI 3715 GACCTCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1498 GGTCACC G^GT[ACGT]ACC
EcoRI 487 GAATTC G^AATTC
EcoRV 1985 GATATC GAT^ATC
NspV 738 TTCGAA TT^CGAA
PaeR7I 3004 CTCGAG C^TCGAG
PflFI 3868 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5236 ATTAAT AT^TAAT
SalI 6022 GTCGAC G^TCGAC
SbfI 3694 CCTGCAGG CCTGCA^GG
SfuI 738 TTCGAA TT^CGAA
SpeI 806 ACTAGT A^CTAGT
SphI 2310 GCATGC GCATG^C
Sse8387I 3694 CCTGCAGG CCTGCA^GG
Tth111I 3868 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3934 TCTAGA T^CTAGA
XhoI 3004 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2675 GGTACC G^GTACC
4884 GGTACC
AccI 3724 GTCTAC GT^[AC][GT]AC
6022 GTCGAC
AccIII 302 TCCGGA T^CCGGA
2370 TCCGGA
AcyI 2541 GGCGCC G[GA]^CG[CT]C
3554 GGCGCC
Alw44I 3009 GTGCAC G^TGCAC
4655 GTGCAC
AocI 1285 CCTGAGG CC^T[ACGT]AGG
4447 CCTGAGG
ApaLI 3009 GTGCAC G^TGCAC
4655 GTGCAC
Asp718I 2675 GGTACC G^GTACC
4884 GGTACC
AviII 81 TGCGCA TGC^GCA
1159 TGCGCA
AvrII 3530 CCTAGG C^CTAGG
4924 CCTAGG
BbeI 2541 GGCGCC GGCGC^C
3554 GGCGCC
BbrPI 635 CACGTG CAC^GTG
911 CACGTG
BcgI 2859 CGAAGGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
405 GCAATGAACTCG
BlnI 3530 CCTAGG C^CTAGG
4924 CCTAGG
BlpI 1736 GCTGAGC GC^T[ACGT]AGC
3340 GCTGAGC
Bpu1102I 1736 GCTGAGC GC^T[ACGT]AGC
3340 GCTGAGC
BsaAI 635 CACGTG [CT]AC^GT[GA]
911 CACGTG
BsaHI 2541 GGCGCC G[GA]^CG[CT]C
3554 GGCGCC
BseAI 302 TCCGGA T^CCGGA
2370 TCCGGA
BspEI 302 TCCGGA T^CCGGA
2370 TCCGGA
Bsu36I 1285 CCTGAGG CC^T[ACGT]AGG
4447 CCTGAGG
CelII 1736 GCTGAGC GC^T[ACGT]AGC
3340 GCTGAGC
DraIII 3012 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4498 CACAGAGTG
Eco81I 1285 CCTGAGG CC^T[ACGT]AGG
4447 CCTGAGG
FspI 81 TGCGCA TGC^GCA
1159 TGCGCA
HaeII 2541 GGCGCC [GA]GCGC^[CT]
3554 GGCGCC
Hin1I 2541 GGCGCC G[GA]^CG[CT]C
3554 GGCGCC
HindIII 589 AAGCTT A^AGCTT
5374 AAGCTT
HpaI 5696 GTTAAC GTT^AAC
5967 GTTAAC
KasI 2541 GGCGCC G^GCGCC
3554 GGCGCC
KpnI 2675 GGTACC GGTAC^C
4884 GGTACC
MfeI 1876 CAATTG C^AATTG
4665 CAATTG
MroI 302 TCCGGA T^CCGGA
2370 TCCGGA
MunI 1876 CAATTG C^AATTG
4665 CAATTG
NarI 2541 GGCGCC GG^CGCC
3554 GGCGCC
PflMI 1758 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3265 CCACGGCATGG
PmaCI 635 CACGTG CAC^GTG
911 CACGTG
PmlI 635 CACGTG CAC^GTG
911 CACGTG
PshAI 306 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4908 GACAGATGTC
SfoI 2541 GGCGCC GGC^GCC
3554 GGCGCC
SspI 796 AATATT AAT^ATT
6084 AATATT
Van91I 1758 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3265 CCACGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI