##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BfrI BglII BlnI BpuAI BsaHI
BseAI BsiWI Bsp1407I BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I CpoI EagI EclXI Eco52I
FseI FspI Hin1I HindIII HpaI
KasI KpnI MfeI MroI MunI
NarI NdeI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1246 GTCTAC GT^[AC][GT]AC
AcsI 2225 GAATTC [GA]^AATT[CT]
AflIII 48 ACGCGT A^C[GA][CT]GT
AhdI 3579 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4220 AGCGCT AGC^GCT
ApoI 2225 GAATTC [GA]^AATT[CT]
AspEI 3579 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2634 CGAGAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1362 TACGTG [CT]AC^GT[GA]
BsaBI 779 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4561 GAGACC GGTCTC(1/5)
BsaWI 2149 ACCGGA [AT]^CCGG[AT]
BsiEI 54 CGGTCG CG[GA][CT]^CG
BsmBI 60 CGTCTC CGTCTC(1/5)
BsmI 4708 GAATGC GAATGC(1/-1)
BspDI 3231 ATCGAT AT^CGAT
ClaI 3231 ATCGAT AT^CGAT
DraI 1207 TTTAAA TTT^AAA
Eam1105I 3579 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4220 AGCGCT AGC^GCT
EcoRI 2225 GAATTC G^AATTC
EcoRV 2568 GATATC GAT^ATC
HincII 3414 GTTGAC GT[CT]^[GA]AC
HindII 3414 GTTGAC GT[CT]^[GA]AC
KspI 3092 CCGCGG CCGC^GG
MamI 779 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 48 ACGCGT A^CGCGT
NaeI 6 GCCGGC GCC^GGC
NgoMIV 6 GCCGGC G^CCGGC
SacII 3092 CCGCGG CCGC^GG
SbfI 3203 CCTGCAGG CCTGCA^GG
ScaI 3327 AGTACT AGT^ACT
Sse8387I 3203 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Asp700I 2639 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3463 GAACAGATTC
BbvCI 2037 GCTGAGG CCTCAGC(-5/-2)
3648 GCTGAGG
BclI 1275 TGATCA T^GATCA
1532 TGATCA
BlpI 2738 GCTAAGC GC^T[ACGT]AGC
3723 GCTGAGC
Bpu1102I 2738 GCTAAGC GC^T[ACGT]AGC
3723 GCTGAGC
BspMI 3707 ACCTGC ACCTGC(4/8)
3965 ACCTGC
BsrDI 3335 CATTGC GCAATG(2/0)
3116 GCAATG
BsrFI 6 GCCGGC [GA]^CCGG[CT]
3767 ACCGGC
BstEII 4838 GGTCACC G^GT[ACGT]ACC
4883 GGTGACC
BstPI 4838 GGTCACC G^GT[ACGT]ACC
4883 GGTGACC
CelII 2738 GCTAAGC GC^T[ACGT]AGC
3723 GCTGAGC
CfoI 2630 GCGC GCG^C
4221 GCGC
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
3767 ACCGGC
DraIII 2605 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2722 CACTGGGTG
DrdI 349 GACGAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3146 GACCCTTCCGTC
EcoNI 2120 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2804 CCTGTCACAGG
EcoO65I 4838 GGTCACC G^GT[ACGT]ACC
4883 GGTGACC
FbaI 1275 TGATCA T^GATCA
1532 TGATCA
HaeII 2629 AGCGCC [GA]GCGC^[CT]
4220 AGCGCT
HhaI 2630 GCGC GCG^C
4221 GCGC
HinP1I 2630 GCGC G^CGC
4221 GCGC
MaeII 1363 ACGT A^CGT
3250 ACGT
MseI 1208 TTAA T^TAA
1874 TTAA
NheI 4223 GCTAGC G^CTAGC
4391 GCTAGC
PaeR7I 3604 CTCGAG C^TCGAG
4291 CTCGAG
SmaI 198 CCCGGG CCC^GGG
3137 CCCGGG
SphI 113 GCATGC GCATG^C
1919 GCATGC
Tru9I 1208 TTAA T^TAA
1874 TTAA
XhoI 3604 CTCGAG C^TCGAG
4291 CTCGAG
XmaCI 198 CCCGGG C^CCGGG
3137 CCCGGG
XmaI 198 CCCGGG C^CCGGG
3137 CCCGGG
XmnI 2639 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3463 GAACAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII