##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BfrI        BglII       BlnI        BpuAI       BsaHI       
BseAI       BsiWI       Bsp1407I    BspEI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     CpoI        EagI        EclXI       Eco52I      
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        MfeI        MroI        MunI        
NarI        NdeI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SspBI       SspI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI         1246  GTCTAC               GT^[AC][GT]AC
AcsI         2225  GAATTC               [GA]^AATT[CT]
AflIII         48  ACGCGT               A^C[GA][CT]GT
AhdI         3579  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      4220  AGCGCT               AGC^GCT
ApoI         2225  GAATTC               [GA]^AATT[CT]
AspEI        3579  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI         2634  CGAGAGAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI        1362  TACGTG               [CT]AC^GT[GA]
BsaBI         779  GATGGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         4561  GAGACC               GGTCTC(1/5)
BsaWI        2149  ACCGGA               [AT]^CCGG[AT]
BsiEI          54  CGGTCG               CG[GA][CT]^CG
BsmBI          60  CGTCTC               CGTCTC(1/5)
BsmI         4708  GAATGC               GAATGC(1/-1)
BspDI        3231  ATCGAT               AT^CGAT
ClaI         3231  ATCGAT               AT^CGAT
DraI         1207  TTTAAA               TTT^AAA
Eam1105I     3579  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     4220  AGCGCT               AGC^GCT
EcoRI        2225  GAATTC               G^AATTC
EcoRV        2568  GATATC               GAT^ATC
HincII       3414  GTTGAC               GT[CT]^[GA]AC
HindII       3414  GTTGAC               GT[CT]^[GA]AC
KspI         3092  CCGCGG               CCGC^GG
MamI          779  GATGGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI           48  ACGCGT               A^CGCGT
NaeI            6  GCCGGC               GCC^GGC
NgoMIV          6  GCCGGC               G^CCGGC
SacII        3092  CCGCGG               CCGC^GG
SbfI         3203  CCTGCAGG             CCTGCA^GG
ScaI         3327  AGTACT               AGT^ACT
Sse8387I     3203  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

Asp700I      2639  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3463  GAACAGATTC          
BbvCI        2037  GCTGAGG              CCTCAGC(-5/-2)
             3648  GCTGAGG             
BclI         1275  TGATCA               T^GATCA
             1532  TGATCA              
BlpI         2738  GCTAAGC              GC^T[ACGT]AGC
             3723  GCTGAGC             
Bpu1102I     2738  GCTAAGC              GC^T[ACGT]AGC
             3723  GCTGAGC             
BspMI        3707  ACCTGC               ACCTGC(4/8)
             3965  ACCTGC              
BsrDI        3335  CATTGC               GCAATG(2/0)
             3116  GCAATG              
BsrFI           6  GCCGGC               [GA]^CCGG[CT]
             3767  ACCGGC              
BstEII       4838  GGTCACC              G^GT[ACGT]ACC
             4883  GGTGACC             
BstPI        4838  GGTCACC              G^GT[ACGT]ACC
             4883  GGTGACC             
CelII        2738  GCTAAGC              GC^T[ACGT]AGC
             3723  GCTGAGC             
CfoI         2630  GCGC                 GCG^C
             4221  GCGC                
Cfr10I          6  GCCGGC               [GA]^CCGG[CT]
             3767  ACCGGC              
DraIII       2605  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2722  CACTGGGTG           
DrdI          349  GACGAGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3146  GACCCTTCCGTC        
EcoNI        2120  CCTTACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2804  CCTGTCACAGG         
EcoO65I      4838  GGTCACC              G^GT[ACGT]ACC
             4883  GGTGACC             
FbaI         1275  TGATCA               T^GATCA
             1532  TGATCA              
HaeII        2629  AGCGCC               [GA]GCGC^[CT]
             4220  AGCGCT              
HhaI         2630  GCGC                 GCG^C
             4221  GCGC                
HinP1I       2630  GCGC                 G^CGC
             4221  GCGC                
MaeII        1363  ACGT                 A^CGT
             3250  ACGT                
MseI         1208  TTAA                 T^TAA
             1874  TTAA                
NheI         4223  GCTAGC               G^CTAGC
             4391  GCTAGC              
PaeR7I       3604  CTCGAG               C^TCGAG
             4291  CTCGAG              
SmaI          198  CCCGGG               CCC^GGG
             3137  CCCGGG              
SphI          113  GCATGC               GCATG^C
             1919  GCATGC              
Tru9I        1208  TTAA                 T^TAA
             1874  TTAA                
XhoI         3604  CTCGAG               C^TCGAG
             4291  CTCGAG              
XmaCI         198  CCCGGG               C^CCGGG
             3137  CCCGGG              
XmaI          198  CCCGGG               C^CCGGG
             3137  CCCGGG              
XmnI         2639  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3463  GAACAGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BciVI       BcnI        BfaI        BglI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII