################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BbeI BbrPI BbsI BfrI BglII BlnI BpuAI BsaHI BseAI BsiWI Bsp1407I BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I CpoI EagI EclXI Eco52I FseI FspI Hin1I HindIII HpaI KasI KpnI MfeI MroI MunI NarI NdeI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 1246 GTCTAC GT^[AC][GT]AC AcsI 2225 GAATTC [GA]^AATT[CT] AflIII 48 ACGCGT A^C[GA][CT]GT AhdI 3579 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4220 AGCGCT AGC^GCT ApoI 2225 GAATTC [GA]^AATT[CT] AspEI 3579 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcgI 2634 CGAGAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 1362 TACGTG [CT]AC^GT[GA] BsaBI 779 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 4561 GAGACC GGTCTC(1/5) BsaWI 2149 ACCGGA [AT]^CCGG[AT] BsiEI 54 CGGTCG CG[GA][CT]^CG BsmBI 60 CGTCTC CGTCTC(1/5) BsmI 4708 GAATGC GAATGC(1/-1) BspDI 3231 ATCGAT AT^CGAT ClaI 3231 ATCGAT AT^CGAT DraI 1207 TTTAAA TTT^AAA Eam1105I 3579 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4220 AGCGCT AGC^GCT EcoRI 2225 GAATTC G^AATTC EcoRV 2568 GATATC GAT^ATC HincII 3414 GTTGAC GT[CT]^[GA]AC HindII 3414 GTTGAC GT[CT]^[GA]AC KspI 3092 CCGCGG CCGC^GG MamI 779 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 48 ACGCGT A^CGCGT NaeI 6 GCCGGC GCC^GGC NgoMIV 6 GCCGGC G^CCGGC SacII 3092 CCGCGG CCGC^GG SbfI 3203 CCTGCAGG CCTGCA^GG ScaI 3327 AGTACT AGT^ACT Sse8387I 3203 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Asp700I 2639 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3463 GAACAGATTC BbvCI 2037 GCTGAGG CCTCAGC(-5/-2) 3648 GCTGAGG BclI 1275 TGATCA T^GATCA 1532 TGATCA BlpI 2738 GCTAAGC GC^T[ACGT]AGC 3723 GCTGAGC Bpu1102I 2738 GCTAAGC GC^T[ACGT]AGC 3723 GCTGAGC BspMI 3707 ACCTGC ACCTGC(4/8) 3965 ACCTGC BsrDI 3335 CATTGC GCAATG(2/0) 3116 GCAATG BsrFI 6 GCCGGC [GA]^CCGG[CT] 3767 ACCGGC BstEII 4838 GGTCACC G^GT[ACGT]ACC 4883 GGTGACC BstPI 4838 GGTCACC G^GT[ACGT]ACC 4883 GGTGACC CelII 2738 GCTAAGC GC^T[ACGT]AGC 3723 GCTGAGC CfoI 2630 GCGC GCG^C 4221 GCGC Cfr10I 6 GCCGGC [GA]^CCGG[CT] 3767 ACCGGC DraIII 2605 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2722 CACTGGGTG DrdI 349 GACGAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3146 GACCCTTCCGTC EcoNI 2120 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2804 CCTGTCACAGG EcoO65I 4838 GGTCACC G^GT[ACGT]ACC 4883 GGTGACC FbaI 1275 TGATCA T^GATCA 1532 TGATCA HaeII 2629 AGCGCC [GA]GCGC^[CT] 4220 AGCGCT HhaI 2630 GCGC GCG^C 4221 GCGC HinP1I 2630 GCGC G^CGC 4221 GCGC MaeII 1363 ACGT A^CGT 3250 ACGT MseI 1208 TTAA T^TAA 1874 TTAA NheI 4223 GCTAGC G^CTAGC 4391 GCTAGC PaeR7I 3604 CTCGAG C^TCGAG 4291 CTCGAG SmaI 198 CCCGGG CCC^GGG 3137 CCCGGG SphI 113 GCATGC GCATG^C 1919 GCATGC Tru9I 1208 TTAA T^TAA 1874 TTAA XhoI 3604 CTCGAG C^TCGAG 4291 CTCGAG XmaCI 198 CCCGGG C^CCGGG 3137 CCCGGG XmaI 198 CCCGGG C^CCGGG 3137 CCCGGG XmnI 2639 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3463 GAACAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BciVI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII