################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI BamHI BcgI BciVI BfrI BsaBI BsiEI BsiWI Bsp1407I BspDI BsrGI BssHII BssSI BstAPI BstBI BstUI Bsu36I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV KpnI KspI MamI MluI MvnI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 1106 TCCGGA T^CCGGA ApaI 265 GGGCCC GGGCC^C AspI 4501 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3576 CTCGGG C^[CT]CG[GA]G AviII 933 TGCGCA TGC^GCA BbeI 5 GGCGCC GGCGC^C BbrPI 1797 CACGTG CAC^GTG BbvCI 79 GCTGAGG CCTCAGC(-5/-2) BclI 53 TGATCA T^GATCA BsaWI 1106 TCCGGA [AT]^CCGG[AT] BseAI 1106 TCCGGA T^CCGGA BsgI 277 GTGCAG GTGCAG(16/14) BsmBI 2201 CGTCTC CGTCTC(1/5) BsmI 4002 GAATGC GAATGC(1/-1) BsoBI 3576 CTCGGG C^[CT]CG[GA]G BspEI 1106 TCCGGA T^CCGGA BstEII 2722 GGTAACC G^GT[ACGT]ACC BstPI 2722 GGTAACC G^GT[ACGT]ACC DraI 1616 TTTAAA TTT^AAA DrdI 1189 GACTTTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 104 CCATGG C^C[GA][CT]GG EcoO65I 2722 GGTAACC G^GT[ACGT]ACC FbaI 53 TGATCA T^GATCA FseI 322 GGCCGGCC GGCCGG^CC FspI 933 TGCGCA TGC^GCA HgaI 2111 GACGC GACGC(5/10) HpaI 1572 GTTAAC GTT^AAC KasI 5 GGCGCC G^GCGCC MroI 1106 TCCGGA T^CCGGA NarI 5 GGCGCC GG^CGCC NcoI 104 CCATGG C^CATGG PflFI 4501 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1797 CACGTG CAC^GTG PmlI 1797 CACGTG CAC^GTG SexAI 1481 ACCTGGT A^CC[AT]GGT SfoI 5 GGCGCC GGC^GCC SnaBI 1789 TACGTA TAC^GTA SpeI 2612 ACTAGT A^CTAGT Tth111I 4501 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3821 TCTAGA T^CTAGA XcmI 110 CCAACCGGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 139 GACGTC GACGT^C 908 GACGTC AccI 816 GTATAC GT^[AC][GT]AC 1792 GTATAC Alw44I 1746 GTGCAC G^TGCAC 1818 GTGCAC ApaLI 1746 GTGCAC G^TGCAC 1818 GTGCAC Asp700I 1391 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3813 GAAAGGTTTC AvrII 2590 CCTAGG C^CTAGG 3120 CCTAGG BanI 5 GGCGCC G^G[CT][GA]CC 287 GGCACC BcnI 114 CCGGG CC^[GC]GG 339 CCGGG BglI 3043 GCCATTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3621 GCCCTTATGGC BlnI 2590 CCTAGG C^CTAGG 3120 CCTAGG BsaAI 1789 TACGTA [CT]AC^GT[GA] 1797 CACGTG BsaI 533 GAGACC GGTCTC(1/5) 2870 GAGACC BspLU11I 455 ACATGT A^CATGT 1946 ACATGT BspMI 2795 ACCTGC ACCTGC(4/8) 4098 ACCTGC BsrBI 1425 CCGCTC CCGCTC(-3/-3) 3985 CCGCTC Bst1107I 816 GTATAC GTA^TAC 1792 GTATAC BstZ17I 816 GTATAC GTA^TAC 1792 GTATAC EcoT22I 1826 ATGCAT ATGCA^T 1836 ATGCAT HaeII 5 GGCGCC [GA]GCGC^[CT] 386 AGCGCC MspA1I 472 CAGCTG C[AC]G^C[GT]G 854 CAGCTG NaeI 323 GCCGGC GCC^GGC 4464 GCCGGC NciI 114 CCGGG CC^[GC]GG 339 CCGGG NdeI 1823 CATATG CA^TATG 3601 CATATG NgoMIV 323 GCCGGC G^CCGGC 4464 GCCGGC NsiI 1826 ATGCAT ATGCA^T 1836 ATGCAT PflMI 1074 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1476 CCAGTACCTGG PstI 2246 CTGCAG CTGCA^G 4103 CTGCAG PvuII 472 CAGCTG CAG^CTG 854 CAGCTG SacI 805 GAGCTC GAGCT^C 987 GAGCTC SmlI 406 CTCAAG C^T[CT][GA]AG 1508 CTCAAG SphI 1828 GCATGC GCATG^C 1838 GCATGC Van91I 1074 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1476 CCAGTACCTGG XmnI 1391 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3813 GAAAGGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BanII BbsI BbvI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspI MunI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII