##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI BamHI BcgI
BciVI BfrI BsaBI BsiEI BsiWI
Bsp1407I BspDI BsrGI BssHII BssSI
BstAPI BstBI BstUI Bsu36I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV KpnI KspI MamI MluI
MvnI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI Sse8387I SspBI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 1106 TCCGGA T^CCGGA
ApaI 265 GGGCCC GGGCC^C
AspI 4501 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3576 CTCGGG C^[CT]CG[GA]G
AviII 933 TGCGCA TGC^GCA
BbeI 5 GGCGCC GGCGC^C
BbrPI 1797 CACGTG CAC^GTG
BbvCI 79 GCTGAGG CCTCAGC(-5/-2)
BclI 53 TGATCA T^GATCA
BsaWI 1106 TCCGGA [AT]^CCGG[AT]
BseAI 1106 TCCGGA T^CCGGA
BsgI 277 GTGCAG GTGCAG(16/14)
BsmBI 2201 CGTCTC CGTCTC(1/5)
BsmI 4002 GAATGC GAATGC(1/-1)
BsoBI 3576 CTCGGG C^[CT]CG[GA]G
BspEI 1106 TCCGGA T^CCGGA
BstEII 2722 GGTAACC G^GT[ACGT]ACC
BstPI 2722 GGTAACC G^GT[ACGT]ACC
DraI 1616 TTTAAA TTT^AAA
DrdI 1189 GACTTTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 104 CCATGG C^C[GA][CT]GG
EcoO65I 2722 GGTAACC G^GT[ACGT]ACC
FbaI 53 TGATCA T^GATCA
FseI 322 GGCCGGCC GGCCGG^CC
FspI 933 TGCGCA TGC^GCA
HgaI 2111 GACGC GACGC(5/10)
HpaI 1572 GTTAAC GTT^AAC
KasI 5 GGCGCC G^GCGCC
MroI 1106 TCCGGA T^CCGGA
NarI 5 GGCGCC GG^CGCC
NcoI 104 CCATGG C^CATGG
PflFI 4501 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1797 CACGTG CAC^GTG
PmlI 1797 CACGTG CAC^GTG
SexAI 1481 ACCTGGT A^CC[AT]GGT
SfoI 5 GGCGCC GGC^GCC
SnaBI 1789 TACGTA TAC^GTA
SpeI 2612 ACTAGT A^CTAGT
Tth111I 4501 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3821 TCTAGA T^CTAGA
XcmI 110 CCAACCGGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 139 GACGTC GACGT^C
908 GACGTC
AccI 816 GTATAC GT^[AC][GT]AC
1792 GTATAC
Alw44I 1746 GTGCAC G^TGCAC
1818 GTGCAC
ApaLI 1746 GTGCAC G^TGCAC
1818 GTGCAC
Asp700I 1391 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3813 GAAAGGTTTC
AvrII 2590 CCTAGG C^CTAGG
3120 CCTAGG
BanI 5 GGCGCC G^G[CT][GA]CC
287 GGCACC
BcnI 114 CCGGG CC^[GC]GG
339 CCGGG
BglI 3043 GCCATTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3621 GCCCTTATGGC
BlnI 2590 CCTAGG C^CTAGG
3120 CCTAGG
BsaAI 1789 TACGTA [CT]AC^GT[GA]
1797 CACGTG
BsaI 533 GAGACC GGTCTC(1/5)
2870 GAGACC
BspLU11I 455 ACATGT A^CATGT
1946 ACATGT
BspMI 2795 ACCTGC ACCTGC(4/8)
4098 ACCTGC
BsrBI 1425 CCGCTC CCGCTC(-3/-3)
3985 CCGCTC
Bst1107I 816 GTATAC GTA^TAC
1792 GTATAC
BstZ17I 816 GTATAC GTA^TAC
1792 GTATAC
EcoT22I 1826 ATGCAT ATGCA^T
1836 ATGCAT
HaeII 5 GGCGCC [GA]GCGC^[CT]
386 AGCGCC
MspA1I 472 CAGCTG C[AC]G^C[GT]G
854 CAGCTG
NaeI 323 GCCGGC GCC^GGC
4464 GCCGGC
NciI 114 CCGGG CC^[GC]GG
339 CCGGG
NdeI 1823 CATATG CA^TATG
3601 CATATG
NgoMIV 323 GCCGGC G^CCGGC
4464 GCCGGC
NsiI 1826 ATGCAT ATGCA^T
1836 ATGCAT
PflMI 1074 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1476 CCAGTACCTGG
PstI 2246 CTGCAG CTGCA^G
4103 CTGCAG
PvuII 472 CAGCTG CAG^CTG
854 CAGCTG
SacI 805 GAGCTC GAGCT^C
987 GAGCTC
SmlI 406 CTCAAG C^T[CT][GA]AG
1508 CTCAAG
SphI 1828 GCATGC GCATG^C
1838 GCATGC
Van91I 1074 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1476 CCAGTACCTGG
XmnI 1391 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3813 GAAAGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII