################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI AocI Aor51HI AscI Asp718I AspEI BamHI BbeI BbvCI BcgI BlpI Bpu1102I BsaHI BsaI BseAI BsiWI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CpoI DraIII DrdI Eam1105I Eco47III Eco81I FauI FseI HaeII HgaI Hin1I HindIII KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Alw44I 2559 GTGCAC G^TGCAC ApaLI 2559 GTGCAC G^TGCAC AseI 1128 ATTAAT AT^TAAT AsnI 1128 ATTAAT AT^TAAT AspI 1365 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 313 TGCGCA TGC^GCA BanI 3195 GGTGCC G^G[CT][GA]CC BbrPI 2556 CACGTG CAC^GTG BcnI 363 CCCGG CC^[GC]GG BglII 4896 AGATCT A^GATCT BsaBI 4 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 5142 CGGCCG CG[GA][CT]^CG BspDI 134 ATCGAT AT^CGAT BspMI 2318 ACCTGC ACCTGC(4/8) BsrDI 3066 CATTGC GCAATG(2/0) BsrFI 956 GCCGGT [GA]^CCGG[CT] BstEII 260 GGTGACC G^GT[ACGT]ACC BstPI 260 GGTGACC G^GT[ACGT]ACC CfoI 314 GCGC GCG^C Cfr10I 956 GCCGGT [GA]^CCGG[CT] ClaI 134 ATCGAT AT^CGAT EagI 5142 CGGCCG C^GGCCG EclXI 5142 CGGCCG C^GGCCG Eco52I 5142 CGGCCG C^GGCCG EcoNI 2210 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 260 GGTGACC G^GT[ACGT]ACC EcoRI 199 GAATTC G^AATTC FspI 313 TGCGCA TGC^GCA HhaI 314 GCGC GCG^C HinP1I 314 GCGC G^CGC MamI 4 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NciI 363 CCCGG CC^[GC]GG NcoI 183 CCATGG C^CATGG NdeI 564 CATATG CA^TATG PflFI 1365 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1048 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2556 CACGTG CAC^GTG PmlI 2556 CACGTG CAC^GTG PshBI 1128 ATTAAT AT^TAAT SapI 2335 GAAGAGC GCTCTTC(1/4) ScaI 2649 AGTACT AGT^ACT SwaI 2977 ATTTAAAT ATTT^AAAT Tth111I 1365 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1048 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Asp700I 89 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4467 GAAACGCTTC AvaI 3502 CTCGAG C^[CT]CG[GA]G 3846 CTCGAG AvrII 1591 CCTAGG C^CTAGG 3318 CCTAGG BbsI 2663 GAAGAC GAAGAC(2/6) 3551 GTCTTC BciVI 207 GGATAC GTATCC(6/5) 3808 GTATCC BglI 179 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1774 GCCCAAAGGGC BlnI 1591 CCTAGG C^CTAGG 3318 CCTAGG BpuAI 2663 GAAGAC GAAGAC(2/6) 3551 GTCTTC BsaAI 2556 CACGTG [CT]AC^GT[GA] 5128 TACGTG BsgI 4705 CTGCAC GTGCAG(16/14) 4493 GTGCAG BsmBI 412 CGTCTC CGTCTC(1/5) 905 GAGACG BsoBI 3502 CTCGAG C^[CT]CG[GA]G 3846 CTCGAG BspHI 1653 TCATGA T^CATGA 2239 TCATGA BstAPI 327 GCACGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3911 GCACTGAATGC BstYI 592 GGATCT [GA]^GATC[CT] 4896 AGATCT DsaI 183 CCATGG C^C[GA][CT]GG 1112 CCGTGG HpaI 2958 GTTAAC GTT^AAC 5202 GTTAAC MflI 592 GGATCT [GA]^GATC[CT] 4896 AGATCT PaeR7I 3502 CTCGAG C^TCGAG 3846 CTCGAG RcaI 1653 TCATGA T^CATGA 2239 TCATGA SacI 786 GAGCTC GAGCT^C 2469 GAGCTC StuI 1686 AGGCCT AGG^CCT 3315 AGGCCT XhoI 3502 CTCGAG C^TCGAG 3846 CTCGAG XhoII 592 GGATCT [GA]^GATC[CT] 4896 AGATCT XmnI 89 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4467 GAAACGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaII BalI BanII BbvI BclI BfaI BfrI BmyI BpmI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BsrGI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoRV EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI