##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        AocI        Aor51HI     
AscI        Asp718I     AspEI       BamHI       BbeI        
BbvCI       BcgI        BlpI        Bpu1102I    BsaHI       
BsaI        BseAI       BsiWI       BspEI       BsrBI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstZ17I     Bsu36I      CelII       CpoI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      FauI        
FseI        HaeII       HgaI        Hin1I       HindIII     
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PinAI       PmeI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Alw44I       2559  GTGCAC               G^TGCAC
ApaLI        2559  GTGCAC               G^TGCAC
AseI         1128  ATTAAT               AT^TAAT
AsnI         1128  ATTAAT               AT^TAAT
AspI         1365  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         313  TGCGCA               TGC^GCA
BanI         3195  GGTGCC               G^G[CT][GA]CC
BbrPI        2556  CACGTG               CAC^GTG
BcnI          363  CCCGG                CC^[GC]GG
BglII        4896  AGATCT               A^GATCT
BsaBI           4  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        5142  CGGCCG               CG[GA][CT]^CG
BspDI         134  ATCGAT               AT^CGAT
BspMI        2318  ACCTGC               ACCTGC(4/8)
BsrDI        3066  CATTGC               GCAATG(2/0)
BsrFI         956  GCCGGT               [GA]^CCGG[CT]
BstEII        260  GGTGACC              G^GT[ACGT]ACC
BstPI         260  GGTGACC              G^GT[ACGT]ACC
CfoI          314  GCGC                 GCG^C
Cfr10I        956  GCCGGT               [GA]^CCGG[CT]
ClaI          134  ATCGAT               AT^CGAT
EagI         5142  CGGCCG               C^GGCCG
EclXI        5142  CGGCCG               C^GGCCG
Eco52I       5142  CGGCCG               C^GGCCG
EcoNI        2210  CCTAAAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       260  GGTGACC              G^GT[ACGT]ACC
EcoRI         199  GAATTC               G^AATTC
FspI          313  TGCGCA               TGC^GCA
HhaI          314  GCGC                 GCG^C
HinP1I        314  GCGC                 G^CGC
MamI            4  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI          363  CCCGG                CC^[GC]GG
NcoI          183  CCATGG               C^CATGG
NdeI          564  CATATG               CA^TATG
PflFI        1365  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1048  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2556  CACGTG               CAC^GTG
PmlI         2556  CACGTG               CAC^GTG
PshBI        1128  ATTAAT               AT^TAAT
SapI         2335  GAAGAGC              GCTCTTC(1/4)
ScaI         2649  AGTACT               AGT^ACT
SwaI         2977  ATTTAAAT             ATTT^AAAT
Tth111I      1365  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1048  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Asp700I        89  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4467  GAAACGCTTC          
AvaI         3502  CTCGAG               C^[CT]CG[GA]G
             3846  CTCGAG              
AvrII        1591  CCTAGG               C^CTAGG
             3318  CCTAGG              
BbsI         2663  GAAGAC               GAAGAC(2/6)
             3551  GTCTTC              
BciVI         207  GGATAC               GTATCC(6/5)
             3808  GTATCC              
BglI          179  GCCACCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1774  GCCCAAAGGGC         
BlnI         1591  CCTAGG               C^CTAGG
             3318  CCTAGG              
BpuAI        2663  GAAGAC               GAAGAC(2/6)
             3551  GTCTTC              
BsaAI        2556  CACGTG               [CT]AC^GT[GA]
             5128  TACGTG              
BsgI         4705  CTGCAC               GTGCAG(16/14)
             4493  GTGCAG              
BsmBI         412  CGTCTC               CGTCTC(1/5)
              905  GAGACG              
BsoBI        3502  CTCGAG               C^[CT]CG[GA]G
             3846  CTCGAG              
BspHI        1653  TCATGA               T^CATGA
             2239  TCATGA              
BstAPI        327  GCACGGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3911  GCACTGAATGC         
BstYI         592  GGATCT               [GA]^GATC[CT]
             4896  AGATCT              
DsaI          183  CCATGG               C^C[GA][CT]GG
             1112  CCGTGG              
HpaI         2958  GTTAAC               GTT^AAC
             5202  GTTAAC              
MflI          592  GGATCT               [GA]^GATC[CT]
             4896  AGATCT              
PaeR7I       3502  CTCGAG               C^TCGAG
             3846  CTCGAG              
RcaI         1653  TCATGA               T^CATGA
             2239  TCATGA              
SacI          786  GAGCTC               GAGCT^C
             2469  GAGCTC              
StuI         1686  AGGCCT               AGG^CCT
             3315  AGGCCT              
XhoI         3502  CTCGAG               C^TCGAG
             3846  CTCGAG              
XhoII         592  GGATCT               [GA]^GATC[CT]
             4896  AGATCT              
XmnI           89  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4467  GAAACGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AflIII      AluI        AlwI        AlwNI       ApaI        
ApoI        AspHI       AvaII       BalI        BanII       
BbvI        BclI        BfaI        BfrI        BmyI        
BpmI        BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspLU11I    BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EarI        EcoO109I    EcoRII      EcoRV       
EcoT14I     EcoT22I     FbaI        Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI