##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
AscI Asp718I AspEI BamHI BbeI
BbvCI BcgI BlpI Bpu1102I BsaHI
BsaI BseAI BsiWI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII CpoI DraIII
DrdI Eam1105I Eco47III Eco81I FauI
FseI HaeII HgaI Hin1I HindIII
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Alw44I 2559 GTGCAC G^TGCAC
ApaLI 2559 GTGCAC G^TGCAC
AseI 1128 ATTAAT AT^TAAT
AsnI 1128 ATTAAT AT^TAAT
AspI 1365 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 313 TGCGCA TGC^GCA
BanI 3195 GGTGCC G^G[CT][GA]CC
BbrPI 2556 CACGTG CAC^GTG
BcnI 363 CCCGG CC^[GC]GG
BglII 4896 AGATCT A^GATCT
BsaBI 4 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5142 CGGCCG CG[GA][CT]^CG
BspDI 134 ATCGAT AT^CGAT
BspMI 2318 ACCTGC ACCTGC(4/8)
BsrDI 3066 CATTGC GCAATG(2/0)
BsrFI 956 GCCGGT [GA]^CCGG[CT]
BstEII 260 GGTGACC G^GT[ACGT]ACC
BstPI 260 GGTGACC G^GT[ACGT]ACC
CfoI 314 GCGC GCG^C
Cfr10I 956 GCCGGT [GA]^CCGG[CT]
ClaI 134 ATCGAT AT^CGAT
EagI 5142 CGGCCG C^GGCCG
EclXI 5142 CGGCCG C^GGCCG
Eco52I 5142 CGGCCG C^GGCCG
EcoNI 2210 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 260 GGTGACC G^GT[ACGT]ACC
EcoRI 199 GAATTC G^AATTC
FspI 313 TGCGCA TGC^GCA
HhaI 314 GCGC GCG^C
HinP1I 314 GCGC G^CGC
MamI 4 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 363 CCCGG CC^[GC]GG
NcoI 183 CCATGG C^CATGG
NdeI 564 CATATG CA^TATG
PflFI 1365 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1048 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2556 CACGTG CAC^GTG
PmlI 2556 CACGTG CAC^GTG
PshBI 1128 ATTAAT AT^TAAT
SapI 2335 GAAGAGC GCTCTTC(1/4)
ScaI 2649 AGTACT AGT^ACT
SwaI 2977 ATTTAAAT ATTT^AAAT
Tth111I 1365 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1048 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 89 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4467 GAAACGCTTC
AvaI 3502 CTCGAG C^[CT]CG[GA]G
3846 CTCGAG
AvrII 1591 CCTAGG C^CTAGG
3318 CCTAGG
BbsI 2663 GAAGAC GAAGAC(2/6)
3551 GTCTTC
BciVI 207 GGATAC GTATCC(6/5)
3808 GTATCC
BglI 179 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1774 GCCCAAAGGGC
BlnI 1591 CCTAGG C^CTAGG
3318 CCTAGG
BpuAI 2663 GAAGAC GAAGAC(2/6)
3551 GTCTTC
BsaAI 2556 CACGTG [CT]AC^GT[GA]
5128 TACGTG
BsgI 4705 CTGCAC GTGCAG(16/14)
4493 GTGCAG
BsmBI 412 CGTCTC CGTCTC(1/5)
905 GAGACG
BsoBI 3502 CTCGAG C^[CT]CG[GA]G
3846 CTCGAG
BspHI 1653 TCATGA T^CATGA
2239 TCATGA
BstAPI 327 GCACGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3911 GCACTGAATGC
BstYI 592 GGATCT [GA]^GATC[CT]
4896 AGATCT
DsaI 183 CCATGG C^C[GA][CT]GG
1112 CCGTGG
HpaI 2958 GTTAAC GTT^AAC
5202 GTTAAC
MflI 592 GGATCT [GA]^GATC[CT]
4896 AGATCT
PaeR7I 3502 CTCGAG C^TCGAG
3846 CTCGAG
RcaI 1653 TCATGA T^CATGA
2239 TCATGA
SacI 786 GAGCTC GAGCT^C
2469 GAGCTC
StuI 1686 AGGCCT AGG^CCT
3315 AGGCCT
XhoI 3502 CTCGAG C^TCGAG
3846 CTCGAG
XhoII 592 GGATCT [GA]^GATC[CT]
4896 AGATCT
XmnI 89 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4467 GAAACGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaII BalI BanII
BbvI BclI BfaI BfrI BmyI
BpmI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BsrGI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoRV
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI